Groval aanalys of the nerve growth using proteomics and superresolution

• Project/Area Number: 18H04013
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Medium-sized Section 46:Neuroscience and related fields
• Research Institution: Niigata University
• Principal Investigator: Igarashi Michihiro 新潟大学, 医歯学系, 教授 (50193173)
• Co-Investigator(Kenkyū-buntansha): 武内 恒成 愛知医科大学, 医学部, 教授 (90206946) ; 中津 史 新潟大学, 医歯学系, 准教授 (50360607)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000); Fiscal Year 2020: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000); Fiscal Year 2019: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000); Fiscal Year 2018: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
• Keywords: 成長円錐 / リン酸化プロテオミクス / 超解像度顕微鏡 / GAP-43 / MAP1B / コンドロイチン硫酸 / JNK / リン酸化 / 脂肪酸合成 / プロテオミクス

Outline of Final Research Achievements

Phosphoproteomic analysis revealed that major phosphorylation sites in the growth cone were mainly phosphorylated by JNK.

Academic Significance and Societal Importance of the Research Achievements

成長円錐の分子マーカー、およびin vivoの神経成長・軸索再生の分子マーカーとして、複数のリン酸化特異抗体を樹立できた。これらはこれまでの同種のものの中で、最も特異性の高いものであり、また成長円錐のシグナル伝達の理解にも大きく貢献できるものとなった。これらの中にはヒトを含む霊長類でも適用可能なものもある。またリン酸化プロテオミクスのビッグデータをどのように解析するか、というスタンダードな方法論を確立できたものと考えている。
超解像度顕微鏡では3次元可視化が可能となって、Z軸方向のイメージング情報が得られるようになった。これらはライブイメージングも可能となった。

Research Products

• [Journal Article] JNK1-Dependent Phosphorylation of GAP-43 Serine 142 is a Novel Molecular Marker for Axonal Growth (2022)
  • Author(s): Okada M, Kawagoe Y, Takasugi T, Nozumi M, Ito Y, Fukusumi H, Kanemura Y, Fujii Y, Igarashi M.
  • Journal Title: Neurochem Res Volume: 印刷中 Issue: 9 Pages: 2668-2682
  • DOI: 10.1007/s11064-022-03580-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PI4P/PS countertransport by ORP10 at ER–endosome membrane contact sites regulates endosome fission (2022)
  • Author(s): Kawasaki A, Sakai A, Nakanishi H, Hasegawa J, Taguchi T, Sasaki J, Arai H, Sasaki T, Igarashi M, Nakatsu F
  • Journal Title: J Cell Biol Volume: 221 Issue: 1 Pages: 1-19
  • DOI: 10.1083/jcb.202103141
  • URL: https://pure.teikyo.jp/en/publications/60ec8c88-daf6-48f2-8f3e-12e4cfbad383
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ChGn-2 plays a cardioprotective role in heart failure caused by acute pressure overload (2021)
  • Author(s): Haryono A, Koji Ikeda, Nugroho DB, Ogata T, Tsuji Y, Matoba S, Moriwaki K, Kitagawa H, Igarashi M, Hirata KI, Emoto N
  • Journal Title: J Am Heart Assoc Volume: - Issue: 7
  • DOI: 10.1161/jaha.121.023401
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Coactosin Promotes F-Actin Protrusion in Growth Cones Under Cofilin-Related Signaling Pathway (2021)
  • Author(s): Hou Xubin、Nozumi Motohiro、Nakamura Harukazu、Igarashi Michihiro、Sugiyama Sayaka
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 9 Pages: 660349-660349
  • DOI: 10.3389/fcell.2021.660349
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chondroitin Sulfate N -acetylgalactosaminyltransferase-2 Impacts Foam Cell Formation and Atherosclerosis by Altering Macrophage Glycosaminoglycan Chain (2021)
  • Author(s): Adhikara Imam Manggalya、Yagi Keiko、Mayasari Dyah Samti、Suzuki Yoko、Ikeda Koji、Ryanto Gusty Rizky Teguh、Sasaki Naoto、Rikitake Yoshiyuki、Nadanaka Satomi、Kitagawa Hiroshi、Miyata Okiko、Igarashi Michihiro、Hirata Ken-ichi、Emoto Noriaki
  • Journal Title: Arteriosclerosis, Thrombosis, and Vascular Biology Volume: 41 Issue: 3 Pages: 1076-1091
  • DOI: 10.1161/atvbaha.120.315789
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinct effects of chondroitin sulfate on hematopoietic cells and the stromal microenvironment in bone marrow hematopoiesis. (2021)
  • Author(s): Takayuki Katagiri, Shun Uemura, Takashi Ushiki, Yaeko Nakajima-Takagi, Motohiko Oshima, Tadahisa Mikami, Asami Kawasaki, Hajime Ishiguro, Tomoyuki Tanaka, Hirohito Sone, Hiroshi Kitagawa, Michihiro Igarashi, Atsushi Iwama, Masayoshi Masuko
  • Journal Title: Experimental hematology Volume: 96 Pages: 52-62
  • DOI: 10.1016/j.exphem.2021.02.003
  • Peer Reviewed
• [Journal Article] Phosphorylation of GAP-43 T172 is a molecular marker of growing axons in a wide range of mammals including primates (2021)
  • Author(s): Okada Masayasu、Kawagoe Yosuke、Sato Yuta、Nozumi Motohiro、Ishikawa Yuya、Tamada Atsushi、Yamazaki Hiroyuki、Sekino Yuko、Kanemura Yonehiro、Shinmyo Yohei、Kawasaki Hiroshi、Kaneko Naoko、Sawamoto Kazunobu、Fujii Yukihiko、Igarashi Michihiro
  • Journal Title: Molecular Brain Volume: 14 Issue: 1 Pages: 66-66
  • DOI: 10.1186/s13041-021-00755-0
  • Peer Reviewed / Open Access
• [Journal Article] Chondroitin sulfate N-acetylgalactosyltransferase-1 knockout shows milder phenotype in experimental autoimmune encephalomyelitis than in wild type (2020)
  • Author(s): Inada Rino、Miyamoto Katsuichi、Tanaka Noriko、Moriguchi Kota、Kadomatsu Kenji、Takeuchi Kosei、Igarashi Michihiro、Kusunoki Susumu
  • Journal Title: Glycobiology Volume: 31 Issue: 3 Pages: 260-265
  • DOI: 10.1093/glycob/cwaa072
  • Peer Reviewed / Open Access
• [Journal Article] Phosphoproteomic and Bioinformatic Methods for Analyzing Signaling in Vertebrate Axon Growth and Regeneration (2020)
  • Author(s): Igarashi M, Kawasaki A, Ishikawa Y, Honda A, Okada M, Okuda S.
  • Journal Title: Journal of Neuroscience Methods Volume: 339 Pages: 108723-108723
  • DOI: 10.1016/j.jneumeth.2020.108723
  • Peer Reviewed / Open Access
• [Journal Article] Neuronal Signaling Involved in Neuronal Polarization and Growth: Lipid Rafts and Phosphorylation (2020)
  • Author(s): Igarashi Michihiro、Honda Atsuko、Kawasaki Asami、Nozumi Motohiro
  • Journal Title: Frontiers in Molecular Neuroscience Volume: 13 Pages: 1-11
  • DOI: 10.3389/fnmol.2020.00150
  • Peer Reviewed / Open Access
• [Journal Article] Isoform-dependent subcellular localization of LMTK1A and LMTK1B and their roles in axon outgrowth and spine formation (2020)
  • Author(s): Wei Ran、Sugiyama Arika、Sato Yuta、Nozumi Motohiro、Nishino Hironori、Takahashi Miyuki、Saito Taro、Ando Kanae、Fukuda Mitsunori、Tomomura Mineko、Igarashi Michihiro、Hisanaga Shin-ichi
  • Journal Title: The Journal of Biochemistry Volume: - Issue: 1 Pages: 23-32
  • DOI: 10.1093/jb/mvaa019
  • NAID: 40022279141
  • Peer Reviewed
• [Journal Article] Microtubule elongation along actin filaments induced by microtubule-associated protein 4 contributes to the formation of cellular protrusions (2020)
  • Author(s): Doki Chihiro、Nishida Kohei、Saito Shoma、Shiga Miyuki、Ogara Hikari、Kuramoto Ayumu、Kuragano Masahiro、Nozumi Motohiro、Igarashi Michihiro、Nakagawa Hiroyuki、Kotani Susumu、Tokuraku Kiyotaka
  • Journal Title: The Journal of Biochemistry Volume: - Issue: 3 Pages: 295-303
  • DOI: 10.1093/jb/mvaa046
  • NAID: 40022375440
  • Peer Reviewed
• [Journal Article] Molecular basis of the functions of the mammalian neuronal growth cone revealed using new methods (2019)
  • Author(s): IGARASHI Michihiro
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 95 Issue: 7 Pages: 358-377
  • DOI: 10.2183/pjab.95.026
  • NAID: 130007689028
  • ISSN: 0386-2208, 1349-2896
  • Year and Date: 2019-07-31
  • Peer Reviewed / Open Access
• [Journal Article] Phosphorylation sites of microtubule-associated protein 1B (MAP1B) involved in axon growth and regeneration (2019)
  • Author(s): Ishikawa Y, Okada M, Honda A, Ito Y, Tamada A, Endo N, Igarashi M
  • Journal Title: Molecular Brain Volume: 12 Issue: 1 Pages: 31711525-31711525
  • DOI: 10.1186/s13041-019-0510-z
  • Peer Reviewed / Open Access
• [Journal Article] Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics (2019)
  • Author(s): Igarashi Michihiro、Okuda Shujiro
  • Journal Title: Molecular Brain Volume: 12 Issue: 1 Pages: 53-53
  • DOI: 10.1186/s13041-019-0476-x
  • Peer Reviewed / Open Access
• [Journal Article] New observations in neuroscience using superresolution microscopy (2018)
  • Author(s): Igarashi Michihiro、Nozumi Motohiro、Wu Ling-Gang、Cella Zanacchi Francesca、Katona Istvan、Barna Laszlo、Xu Pingyong、Zhang Mingshu、Xue Fudong、Boyden Edward
  • Journal Title: The Journal of Neuroscience Volume: 38 Issue: 44 Pages: 9459-9467
  • DOI: 10.1523/jneurosci.1678-18.2018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Craniofacial abnormality with skeletal dysplasia in mice lacking chondroitin sulfate N-acetylgalactosaminyltransferase-1 (2018)
  • Author(s): Ida-Yonemochi Hiroko、Morita Wataru、Sugiura Nobuo、Kawakami Ryosuke、Morioka Yuki、Takeuchi Yuka、Sato Toshiya、Shibata Shunichi、Watanabe Hideto、Imamura Takeshi、Igarashi Michihiro、Ohshima Hayato、Takeuchi Kosei
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 17134-17134
  • DOI: 10.1038/s41598-018-35412-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Growth cone phosphoproteomics reveals that GAP-43 phosphorylated by JNK is a marker of axon growth and regeneration. (2018)
  • Author(s): Okada M*, Kawasaki A*, Tamada A*, Okuda S, Nozumi M, Ito Y, Kobayashi D, Yamasaki T, Yokoyama R, Shibata T, Nishina H, Yoshida Y, Fujii Y, Takeuchi K, Igarashi M(*These three authorscontributed equally.).
  • Journal Title: ISCIENCE Volume: 4 Pages: 190-203
  • DOI: 10.1016/j.isci.2018.05.019
  • Peer Reviewed / Open Access
• [Journal Article] Vesicular movements in the growth cone (2017)
  • Author(s): Motohiro Nozumi, Michihiro Igarashi
  • Journal Title: Neurochemistry International Volume: 印刷中 Pages: 71-76
  • DOI: 10.1016/j.neuint.2017.09.011
  • Peer Reviewed
• [Journal Article] Roles of CSGalNAcT1, a key enzyme in regulation of CS synthesis, in neuronal regeneration and plasticity (2017)
  • Author(s): Igarashi M, Takeuchi K, Sugiyama S.
  • Journal Title: Neurochem Int. Volume: 17 Pages: 0197-0186
  • DOI: 10.1016/j.neuint.2017.10.001
  • Peer Reviewed / Open Access
• [Presentation] 蛍光陰影によるオルガネラのライブイメージング (2022)
  • Author(s): 野住 素広、五十嵐 道弘、Valentin Nagerl
  • Organizer: 生体運動研究合同会議
• [Presentation] 神経成長円錐の３次元空間で生じるアクチン再編と形質膜取込み (2021)
  • Author(s): 野住 素広、五十嵐 道弘
  • Organizer: 第126回日本解剖学会総会・全国学術集会
  • Invited
• [Presentation] 非接着性のフィロポディアは拡散性ガイダンス因子の濃縮に関係する (2021)
  • Author(s): 野住 素広、五十嵐 道弘
  • Organizer: 第44回日本神経科学大会
• [Presentation] 組織透明化による脳の発達と神経再生の解析 (2021)
  • Author(s): 川越 洋介、岡田 正康、田井中 一貴、五十嵐 道弘
  • Organizer: 第44回日本神経科学大会
• [Presentation] 霊長類と齧歯類における神経成長マーカーの探索 (2021)
  • Author(s): 岡田 正康、川越 洋介、金子 奈穂子、山崎 博幸、河崎 洋志、棗田 学、大石 誠、藤井 幸彦、五十嵐 道弘
  • Organizer: 第44回日本神経科学大会
• [Presentation] 超解像陰影法による複数オルガネラの同時可視化 (2021)
  • Author(s): 野住 素広、五十嵐 道弘
  • Organizer: 第64回日本神経化学会大会
• [Presentation] Phosphorylation of GAP-43 T172 is a molecular marker representing the growing axons in a wide range of mammals including primates (2021)
  • Author(s): 岡田 正康、河嵜麻実、金子 奈穂子、山崎 博幸、河崎 洋志、金村米博、澤本和延、藤井幸彦、五十嵐 道弘
  • Organizer: 第64回日本神経化学会大会
• [Presentation] ヒト神経細胞成長マーカーの同定 (2021)
  • Author(s): 岡田正康、金子奈穂子、山崎博幸、棗田学、大石誠、金村米博、澤本和延、藤井幸彦、五十嵐道弘
  • Organizer: 第80回日本脳神経外科学会総会
  • Invited
• [Presentation] 神経軸索成長における極長鎖脂肪酸産生酵素GPSN2の生理的役割 (2021)
  • Author(s): 本多 敦子、野住 素広、五十嵐 道弘
  • Organizer: 第94回日本生化学大会
• [Presentation] 成長円錐プロテオミクスから発見した新規神経成長・再生マーカー (2020)
  • Author(s): 岡田正康、河嵜麻実、玉田篤史、佐藤勇太、北浦弘樹、棗田学、木村展之、金子奈穂子、武内恒成、柿田明美、藤井幸彦、五十嵐道弘
  • Organizer: 第43回日本神経科学大会
  • Invited
• [Presentation] 神経発生における極長鎖脂肪酸合成酵素GPSN2の生理機能 (2020)
  • Author(s): 本多敦子、野住素広、内野春希、有田誠、五十嵐道弘
  • Organizer: 第43回日本神経科学大会
• [Presentation] 組織透明化後免疫染色によるマウス胎児脳の解析 (2020)
  • Author(s): 深沢洋介、岡田正康、田井中一貴、五十嵐道弘
  • Organizer: 第43回日本神経科学大会
• [Presentation] 神経発生過程における脂肪酸鎖長伸長酵素GPSN2の生理的役割 (2020)
  • Author(s): 本多敦子、野住素広、内野春希、有田誠、五十嵐道弘
  • Organizer: 第93回日本生化学会大会
• [Presentation] Omics studies of axon development and regeneration (2020)
  • Author(s): Michihiro Igarashi
  • Organizer: Metabolites in neuronal regeneration
  • Int'l Joint Research / Invited
• [Presentation] F-アクチンは神経成長円錐の非接着面における軸索ガイダンス分子の捕捉に関与する (2020)
  • Author(s): 野住素広、五十嵐道弘
  • Organizer: 第43回日本分子生物学会年会
• [Presentation] 超解像陰影法（SUSHI）による細胞内構造の可視化 (2020)
  • Author(s): 15. 野住素広、V. Nägerl、五十嵐道弘
  • Organizer: 生体運動研究合同班会議2020（京都）
• [Presentation] A molecular marker in neural development and plasticity in primates (2020)
  • Author(s): 16..M. Okada, Y. Sato, A. Kawasaki, M. Natsumeda, M. Oishi, K. Sawamoto, Y. Fujii, M. Igarashi
  • Organizer: AMED-CREST国際シンポジウム（宮崎）
• [Presentation] JNK dependent phosphorylation sites of GAP-43 is involved in axon growth/regeneration, revealed by growth cone phosphoproteomics (2020)
  • Author(s): 17.A. Kawasaki, A. Tamada, M. Okada, S. Okuda, M. Igarashi
  • Organizer: AMED-CREST国際シンポジウム（宮崎）
• [Presentation] Super-resolution microscopy reveals F-actin-mediated mechanisms of nerve growth and maintenance (2020)
  • Author(s): 18.M. Nozumi，Y. Sato，M. Igarashi
  • Organizer: AMED-CREST国際シンポジウム（宮崎）
• [Presentation] Phosphoproteomics analysis of the growth cone reveals the new molecular markers of growing axons. (2019)
  • Author(s): 1. M. Igarashi
  • Organizer: 7th Mediterranean Neuroscience Conference (Marrakech, Morocco)
  • Int'l Joint Research / Invited
• [Presentation] 新規・小胞体-エンドソーム膜接触部位における脂質交換輸送機構とその生理機能の解明 (2019)
  • Author(s): 2. 河嵜麻実、酒井晶子、荒川聡子、中西正樹、佐々木雄彦、清水重臣、五十嵐道弘、中津史
  • Organizer: 第71回日本生物細胞学会大会（神戸）
  • Invited
• [Presentation] 成長円錐のリン酸化プロテオミクスにより同定されたJNK基質の軸索伸長における役割 (2019)
  • Author(s): 3. 河嵜麻実、高杉俊之、玉田篤史、奥田修二郎、崎村建司、仁科博史、五十嵐道弘
  • Organizer: 第92回日本生化学会大会（横浜）
  • Invited
• [Presentation] Lipoquality and development of the GPSN2 knockout mice (2019)
  • Author(s): 4.M. Igarashi, A. Honda, M. Nozumi
  • Organizer: 60th International Conference on the Bioscience of Lipids. (Tokyo)
  • Int'l Joint Research
• [Presentation] 成長円錐のリン酸化プロテオミクスにより同定されたGAP-43のJNKリン酸化部位は伸長または再生する神経軸索で活性化される (2019)
  • Author(s): 5. 河嵜麻実、玉田篤史、岡田正康、奥田修二郎、仁科博史、山崎世和、五十嵐道弘
  • Organizer: 第42回日本神経科学・第62回日本神経化学合同大会（新潟）
• [Presentation] 高頻度チロシンリン酸化MAP１Bの神経発生過程における機能解析 (2019)
  • Author(s): 6. 伊藤泰行、本多敦子、五十嵐道弘
  • Organizer: 第42回日本神経科学・第62回日本神経化学合同大会（新潟）
• [Presentation] 発生脳におけるMAP１Bの高頻度リン酸化部位 (2019)
  • Author(s): 7. 石川裕也、本多敦子、伊藤泰行、河嵜麻実、玉田篤史、遠藤直人、五十嵐道弘
  • Organizer: 第42回日本神経科学・第62回日本神経化学合同大会（新潟）
• [Presentation] Evolutional analysis of the protein phosphorylation sites in the growth cone (2019)
  • Author(s): 8. M. Igarashi, S. Okuda
  • Organizer: 2019 ISN-ASN Meeting (Montreal, CA)
  • Int'l Joint Research
• [Presentation] 高頻度チロシンリン酸化MAP１Bの神経発生過程における機能解析 (2019)
  • Author(s): 9. 伊藤泰行、本多敦子、五十嵐道弘
  • Organizer: 第92回日本生化学会大会（横浜）
• [Presentation] Phosphorylation of MAP1B in the growing/ regrowing axon (2019)
  • Author(s): 10. 本多敦子、石川裕也、五十嵐道弘
  • Organizer: 第92回日本生化学会大会（横浜）
• [Presentation] The MAP1B phosphorylation sites specifically localized in the developing brain and in the regenerating peripheral nerves (2019)
  • Author(s): 11. Y. Ishikawa, M. Okada, A. Honda, Y. Ito, A. Tamada, N. Endo, M. Igarashi
  • Organizer: SFN annual Meeting 2019, (Chicago, USA)
  • Int'l Joint Research
• [Presentation] 神経成長円錐のタンパク質リン酸化に関する進化のバイオインフォマティクス解析 (2019)
  • Author(s): 12. 五十嵐道弘、奥田修二郎
  • Organizer: 第42回日本分子生物学会（福岡）
• [Presentation] 脳発生過程で高頻度にリン酸化されるMAP１Bチロシン残基の同定と機能解析 (2019)
  • Author(s): 13..伊藤泰行、本多敦子、五十嵐道弘
  • Organizer: 第42回日本分子生物学会（福岡）
• [Presentation] Phosphoproteomic analysis of the mammalian growth cones reveals the novel molecular markers of the growing/regenerating axons, specifically to vertebrate (2019)
  • Author(s): 14.M. Igarashi, Y. Ishikawa, S. Okuda
  • Organizer: ASCB EMBO 2019 Meeting (Washington, DC, USA)
  • Int'l Joint Research
• [Presentation] コンドロイチン硫酸(CS)合成酵素のノックアウトマウス解析 (2019)
  • Author(s): 五十嵐 道弘
  • Organizer: 先端動物支援プラットフォーム成果報告会
  • Invited
• [Presentation] 3D analysis of the intracellular structures in growth cones using superresolution microscopy (2019)
  • Author(s): M. Igarashi
  • Organizer: EMBO Molecular Neuroscience
  • Invited
• [Presentation] 哺乳動物成長円錐の分子基盤をどう理解するか？ (2018)
  • Author(s): 五十嵐 道弘
  • Organizer: 生理学研究所シンポジウム
  • Invited
• [Presentation] New observations in neuroscience using superresolution microscopy. (2018)
  • Author(s): M. Igarashi
  • Organizer: Soceity for Neuroscience
  • Int'l Joint Research / Invited
• [Presentation] New relationships between F-actin organization and memberance in the growth cone revealed by SIM (2018)
  • Author(s): M. Nozumi, M. Igarashi
  • Organizer: Soceity for Neuroscience
  • Int'l Joint Research / Invited
• [Presentation] 構造化照明による超解像顕微鏡（SIM）で明らかになった神経突起先端の3次元構造 (2018)
  • Author(s): 野住 素広、五十嵐 道弘
  • Organizer: 日本分子生物学会大会
  • Int'l Joint Research
• [Presentation] 神経細胞の形態形成シグナリングにおけるGPM6aのパルミトイル化と脂質ラフト集積の意義 (2018)
  • Author(s): 五十嵐 道弘
  • Organizer: 日本神経化学会大会
  • Invited
• [Presentation] Abnormalities in perineuronal nets (PNN) and behaviors in mice without CSGalNAcT1 (2018)
  • Author(s): M. Igarashi
  • Organizer: Glycobiology 2018
  • Int'l Joint Research / Invited
• [Presentation] Phosphoproteomics of the mammalian growth cone reveals that phosphorylation of GAP-43 by JNK is specific to the (2018)
  • Author(s): M. Igarashi
  • Organizer: American Society for Cell Biology
  • Invited
• [Presentation] Phosphorylation profiles of neuronal growth cones revealed by phosphoproteomics (2018)
  • Author(s): M. Igarashi
  • Organizer: Gordon Conference
  • Int'l Joint Research
• [Presentation] Growth cone phosphoproteomics reveals that JNK-dependent phosphorylation of GAP-43 is specific to the growing axon (2018)
  • Author(s): A. Kawasaki, M. Igarash
  • Organizer: Cold Spring Harbor Asaia Symposium
  • Int'l Joint Research
• [Presentation] JNK-dependent phosphorylation sites of GAP-43 is a marker of axon growth/regeneration, revealed by growth cone phosphoproreomics (2018)
  • Author(s): A. Kawasaki, M.. Igarashi
  • Organizer: Society for Neuroscience
  • Int'l Joint Research
• [Remarks] 新潟大学大学院医歯学総合研究科分子細胞機能学
  • URL: https://www.med.niigata-u.ac.jp/bc2/