| Budget Amount *help |
¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000)
Fiscal Year 2021: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2020: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2019: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2018: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
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| Outline of Final Research Achievements |
To develop a technique and basic understandings for high reproducible and efficient preparation of organoids, i.e., the budding structures of artificial small organs formed in a culture environment, we systematically investigated the effects of parameters of viscoelastic properties and shape-inducing curvature of scaffolds for organoid culture. The rheological properties of Matrigel, which is commonly used in conventional organoid culture, were modulated by additional cross-linking and mechanical fragmentation of the matrix to determine the optimal viscoelastic property of the matrix for organoid formation. A simple synthetic polymer, PEG, was physically crosslinked with modified polypeptides, and the determined optimal viscoelastic properties were introduced to a laminin-loaded system through regulating the strength of physical crosslinking. This synthetic polymer matrix successfully produced liver organoids more stable than in the Matrigel system.
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