Spectral opponency in photoreceptors : neuroethological analysis

• Project/Area Number: 18H05273
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Review Section: Broad Section G
• Research Institution: The Graduate University for Advanced Studies
• Principal Investigator: 蟻川 謙太郎 総合研究大学院大学, 先導科学研究科, 教授 (20167232)
• Co-Investigator(Kenkyū-buntansha): 松下 敦子 総合研究大学院大学, 先導科学研究科, 講師 (50450416) ; 木下 充代 総合研究大学院大学, 先導科学研究科, 准教授 (80381664)
• Project Period (FY): 2018-06-11 – 2023-03-31
• Project Status: Granted (Fiscal Year 2021)
• Budget Amount: ¥200,200,000 (Direct Cost: ¥154,000,000、Indirect Cost: ¥46,200,000); Fiscal Year 2021: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2020: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2019: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2018: ¥119,340,000 (Direct Cost: ¥91,800,000、Indirect Cost: ¥27,540,000)
• Keywords: 昆虫 / 色覚 / 視細胞 / 視葉板 / 波長対比性

Outline of Annual Research Achievements

私たちは、昆虫の色覚神経機構とその進化に関する研究の一環として、視葉板における視細胞間シナプスに着目している。視細胞間シナプスは恐らく抑制性で、ナミアゲハで我々が初めて発見したものである。優れた色覚をもつアゲハは色覚研究のモデル種で、複眼には分光感度の違う６種の視細胞がある。その視細胞が抑制し合うと何が起こり、 二次ニューロンであるLMCには何が伝達されるのか？　この側枝とシナプスはショウジョウバエにはない。それは、ハエでは視葉板に入る視細胞の分光感度がみな同じで、視葉板と色覚の関係が薄いためと考えられる。アゲハ視細胞の長い側枝と視細胞間シナプスは色覚神経機構の重要な要素と考え、本研究では視葉板における波長情報処理の機構を詳しく調べている。アゲハについては、１）視細胞の波長対比性と、２）LMCにおける波長情報処理を、また色覚の進化を解明するために、３）視葉板回路の比較研究も進めている。
１については、昨年度に引き続き８種の波長対比性を細かく解析した。２ではLMCに少なくとも３種の分光感度を同定、１の結果と合わせて記載的論文を出版した。CRISPR-Cas9法でチャネルノックアウトを試みた個体では、予想どおりチャネルの発現量低下が、免疫組織化学で確認できた。２の過程で、動き感受性ニューロンの反応に波長依存性があることを発見した。実は我々はアゲハは動き知覚に色情報を使っているという行動実験の結果を得ており、これは、動き知覚はモノクロという定説を覆すものである。今回の結果は行動の現象ともよく合う。動き知覚と色覚は表裏一体の関係にあるため、この発見は本研究を予想以上に進展させる可能性を秘めた、画期的なものと考えている。３に関しては、ホウジャク、モンシロチョウ、ウラギンシジミ、ヤマトシジミ、モンキアゲハ、ネッタイシマカ、ミカンキイロアザミウマについて、視葉板の連続電顕像を取得した。

Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

生理学、分子生物学、解剖学の実験は予定通り進展している。
新しく赴任したポスドクと始めた生理学実験から、動き感受性ニューロン反応の波長依存性が見つかった。これは予想していなかった新しい展開につながる可能性が高く、とくに注目している。

Strategy for Future Research Activity

本研究で推進する研究項目は、１）視細胞波長対比性、２）LMCにおける波長情報処理、３）視葉板カートリッジ構造の多様性である。１と２については引き続き、電気生理と遺伝子編集の方法で実験を進める。特に２では動き知覚の色感受性にも焦点を当て、色覚系を広く深く理解できるような実験を進める。３ではSBF-SEMを駆使してアゲハ以外の昆虫で視葉板構造を解明するほか、遺伝子編集アゲハ個体の視葉板構造も解析する。

Research Products

• [Int'l Joint Research] University of Ljubljana(スロベニア)
• [Int'l Joint Research] IISER Trivandram(インド)
• [Int'l Joint Research] New York University/University of Maryland Baltimore County/University of Cincinnati(米国)
• [Int'l Joint Research] University of Wuerzburg(ドイツ)
• [Int'l Joint Research] University of Oulu(フィンランド)
• [Int'l Joint Research] University of Ljubljana(スロベニア)
• [Journal Article] Photoreceptor projection from a four‐tiered retina to four distinct regions of the first optic ganglion in a jumping spider (2019)
  • Author(s): Nagata Takashi、Arikawa Kentaro、Kinoshita Michiyo
  • Journal Title: Journal of Comparative Neurology Volume: 527 Pages: 1348-1361
  • DOI: 10.1002/cne.24584
  • Peer Reviewed
• [Journal Article] Compound eyes of the small white butterfly Pieris rapae have three distinct classes of red photoreceptors (2019)
  • Author(s): Blake AJ, Pirih P, Qiu X, Arikawa K, Gries G (2019)
  • Journal Title: Journal of Comparative Physiology A Volume: 205 Pages: 553-565
  • DOI: 10.1007/s00359-019-01330-8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Spectral tuning mediated by helix III in butterfly long wavelength-sensitive visual opsins revealed by heterologous action spectroscopy. (2019)
  • Author(s): Saito T, Koyanagi M, Sugihara T, Nagata T, Arikawa K, Terakita A
  • Journal Title: Zoological Letters Volume: 5 Pages: 1-11
  • DOI: 10.1186/s40851-019-0150-2
  • Peer Reviewed / Open Access
• [Journal Article] Chromatic information processing in the first optic ganglion of the butterfly Papilio xuthus. (2019)
  • Author(s): Chen, P.-J., Belusic, G., Arikawa, K. (2019).
  • Journal Title: Journal of Comparative Physiology A Volume: 206 Pages: 199-216
  • DOI: 10.1007/s00359-019-01390-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Monopolatic motion vision in the butterfly Papilio xuthus (2019)
  • Author(s): Finlay J Stewart, Michiyo Kinoshita, Kentaro Arikawa
  • Journal Title: The Journal of Experimental Biology Volume: 222
  • DOI: 10.1242/jeb.191957
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immunolocalization suggests a role of the histamine-gated chloride channel PxHCLB in spectral opponent processing in butterfly photoreceptors (2019)
  • Author(s): Chen Pei-Ju、Matsushita Atsuko、Wakakuwa Motohiro、Arikawa Kentaro
  • Journal Title: Journal of Comparative Neurology Volume: 527 Pages: 753-766
  • DOI: 10.1002/cne.24558
  • Peer Reviewed
• [Journal Article] 昆虫色覚研究の歴史とこれから (2019)
  • Author(s): 蟻川謙太郎
  • Journal Title: 日本色彩学会誌 Volume: 43 Pages: 215-218
• [Journal Article] Non-linear amplification of graded voltage signals in the first-order visual interneurons of the butterflyPapilio xuthus (2018)
  • Author(s): Rusanen Juha、Frolov Roman、Weckstroem Matti、Kinoshita Michiyo、Arikawa Kentaro
  • Journal Title: The Journal of Experimental Biology Volume: 221
  • DOI: 10.1242/jeb.179085
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The More, the Better? A Butterfly with 15 Kinds of Light Sensors in Its Eye (2018)
  • Author(s): Pei-Ju Chen, Finlay J Stewart, Kentaro Arikawa
  • Journal Title: Frontiers for Young Minds Volume: 6 Pages: 70-70
  • DOI: 10.3389/frym.2017.00070
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The giant butterfly-moth Paysandisia archon has spectrally rich apposition eyes with unique light-dependent photoreceptor dynamics (2018)
  • Author(s): Pirih Primoz、Ilic Marko、Rudolf Jerneja、Arikawa Kentaro、Stavenga Doekele G.、Belusic Gregor
  • Journal Title: Journal of Comparative Physiology A Volume: 204 Pages: 639-651
  • DOI: 10.1007/s00359-018-1267-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Physiological responses of ionotropic histamine receptors, PxHCLA and PxHCLB, to neurotransmitter candidates in a butterfly, Papilio xuthus (2018)
  • Author(s): Akashi Hiroshi D.、Chen Pei-Ju、Akiyama Tokiho、Terai Yohey、Wakakuwa Motohiro、Takayama Yasunori、Tominaga Makoto、Arikawa Kentaro
  • Journal Title: J Exp Biol Volume: 221 Pages: 2234-2242
  • DOI: 10.1242/jeb.183129
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Physiology of butterfly color vision (2020)
  • Author(s): Arikawa K
  • Organizer: Special seminar at the Department of Biology at the Indian Institute of Science Education and Research Trivandrum
  • Int'l Joint Research / Invited
• [Presentation] Butterfly color vision - Another view of our world (2020)
  • Author(s): Arikawa K
  • Organizer: The 2nd International Symposium for Color Science and Art (postponed)
  • Int'l Joint Research / Invited
• [Presentation] チョウが見る色の世界―昆虫視覚の神経行動学 (2019)
  • Author(s): 蟻川謙太郎
  • Organizer: 第35回国際生物学賞記念シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Butterfly vision - another view of our world (2019)
  • Author(s): Arikawa K
  • Organizer: International Conference on Biodiversity 2019
  • Int'l Joint Research / Invited
• [Presentation] Another view of our world - Neuroethology of butterfly color vision (2019)
  • Author(s): Arikawa K
  • Organizer: Special seminar at the Biological Sciences Department at the University of Cincinnati
  • Int'l Joint Research / Invited
• [Presentation] Exploring the butterfly lamina (2019)
  • Author(s): Arikawa K
  • Organizer: Special seminar at the Department of Biology at the University of New York
  • Int'l Joint Research / Invited
• [Presentation] “Colorful” lamina of Papilio (2019)
  • Author(s): Arikawa K
  • Organizer: Special seminar at the Biological Sciences Department at the University of Maryland Baltimore County
  • Int'l Joint Research / Invited
• [Presentation] Spectral information processing in the lamina of a butterfly, Papilio xuthus (2019)
  • Author(s): Arikawa K, Chen P-J, Belusic G, Kinoshita M, Matsushita A, Stewart FJ
  • Organizer: Janelia Conference “Color vision: Circuits and Behavior”
  • Int'l Joint Research / Invited
• [Presentation] チョウ類の交尾行動と産卵行動を制御する紫外線受容器 (2019)
  • Author(s): 蟻川謙太郎、塚本寿夫
  • Organizer: シンポジウム「非視覚タイプの光環境応答とメカニズム」，日本動物学会第90回大会
  • Invited
• [Presentation] The roles of colour and polarization contrast for motion detection in Papilio butterflies (2019)
  • Author(s): Stewart F, Kinoshita M, Arikawa K
  • Organizer: 4th International Conference on Invertebrate Vision
  • Int'l Joint Research
• [Presentation] Four ventral ommatidial types, perhaps? Optical retinography (ORG) in the Angled Sunbeam butterfly (Curetis acuta) (2019)
  • Author(s): Pirih P, Arikawa K
  • Organizer: 4th International Conference on Invertebrate Vision
  • Int'l Joint Research
• [Presentation] Light music for the masses: Polychrome stimulation of Papilio xuthus photoreceptors with the LED synthesizer (2019)
  • Author(s): Pirih P, Ilic; M, Meglic A, Arikawa K, Belusic G
  • Organizer: 4th International Conference on Invertebrate Vision
  • Int'l Joint Research
• [Presentation] The compound eye of a nymphalid migrator the Chestnut Tiger butterfly, Parantica sita (2019)
  • Author(s): Nagloo N, Arikawa K, Kinoshita M
  • Organizer: 4th International Conference on Invertebrate Vision
  • Int'l Joint Research
• [Presentation] Color vision see at the lamina of a butterfly, Papilio xuthus (2019)
  • Author(s): Arikawa K, Matsushita A, Stewart F, Chen P-J, Ilic; M, Kinoshita M, Belusic; G
  • Organizer: 4th International Conference on Invertebrate Vision
  • Int'l Joint Research / Invited
• [Presentation] 農業害虫アザミウマ類の波長選好性 (2019)
  • Author(s): 大木碩仁、荻野拓海、山口照美、野呂 知加子、戒能洋一、蟻川謙太郎、霜田政美
  • Organizer: 令和元年度 農業・工業原材料生産と光技術研究会
• [Presentation] アザミウマ４種の波長選好性の特徴について (2019)
  • Author(s): 大木碩仁、荻野拓海、山口照美、野呂知加子、戒能洋一、蟻川謙太郎、霜田政美
  • Organizer: 天敵利用研究会
• [Presentation] Comparative analysis of the number of Kenyon cell subtype in the mushroom bodies of the brain among various insect species (2019)
  • Author(s): Suzuki A, Kinoshita M, Mukai A Oguchi K, Mizunami M, Arikawa K, Goto S, Miura T, Kubo T,
  • Organizer: The 41st Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
• [Presentation] Modeling of lateral inhibition in the lamina as the basis of acute color vision in a butterfly, Papilio xuthus. (2019)
  • Author(s): Ilic M, Arikawa K
  • Organizer: The 41st Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
• [Presentation] 様々な昆虫種における脳キノコ体ケニヨン細胞サブタイプの探索と比較解析 (2019)
  • Author(s): 鈴木 敦己, 木下 充代, 向井 歩, 小口 晃平, 水波 誠, 蟻川 謙太郎, 後藤 慎介, 三浦 徹, 久保 健雄
  • Organizer: 第42回分子生物学会
• [Presentation] チョウの生殖行動を制御する光受容体 (2018)
  • Author(s): 塚本寿夫、内山博允、秋山辰穂、小柳光正、古谷祐詞、寺北明久、蟻川謙太郎
  • Organizer: 日本動物学会第89回大会
  • Invited
• [Presentation] アゲハ視葉板カートリッジ間の個眼タイプ特異的接続：連続ブロックフェイス走査電顕(SBF-SEM)による解析 (2018)
  • Author(s): 松下敦子、Finlay Stewart、宮崎直幸、村田和義、蟻川謙太郎
  • Organizer: 日本動物学会第89回大会
• [Presentation] Investigations into the mechanism of polarized light sensitivity in Pieris rapae (2018)
  • Author(s): Blake AJ, Kwok S, Hahn GS, Qiu X, Arikawa K, Gries G
  • Organizer: 2018 ESA, ESC and ESBC Joint Annual Meeting
  • Int'l Joint Research
• [Presentation] Inside the compound eye of a migratory butterfly, Parantica sita (2018)
  • Author(s): Nagloo N, Arikawa K, Kinoshita M
  • Organizer: The 40th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
• [Presentation] Neuronal circuitry for achromatic and chromatic vision in the Papilio lamina (2018)
  • Author(s): Chen P-J, Belusic G, Matsushita A, Arikawa K
  • Organizer: The 40th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
• [Presentation] Ommatidial type-specific intra- and inter-cartridge connections in the Papilio lamina revealed by serial block face-scanning electron microscopy (SBF-SEM) (2018)
  • Author(s): Arikawa K, Stewart F, Miyazaki N, Murata K, Matsushita A: Ommatidial type-specific intra- and inter-cartridge connections in the Papilio lamina revealed by serial block face-scanning electron microscopy (SBF-SEM).
  • Organizer: The 13th International Congress of Neuroethology
  • Int'l Joint Research
• [Presentation] The retinal organization of a migratory butterfly Parantica sita (2018)
  • Author(s): Nagloo N, Arikawa K, Kinoshita M
  • Organizer: The 13th International Congress of Neuroethology
  • Int'l Joint Research
• [Presentation] Examination of the histamine hypothesis for a mechanism underlying photoreceptor spectral opponency in the Papilio butterfly (2018)
  • Author(s): Chen P-J, Belusic G, Matsushita A, Arikawa K
  • Organizer: The 13th International Congress of Neuroethology
  • Int'l Joint Research
• [Book] 動物学の百科事典（「感覚系の構造と機能」執筆） (2018)
  • Author(s): 蟻川謙太郎（公益社団法人日本動物学会、分担執筆）
  • Total Pages: 800
  • Publisher: 丸善出版
  • ISBN: 9784621303092