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Construction of evaluation system of property control and taste response of novel rice starch aimed at utilization of rice higher-order function

Research Project

Project/Area Number 18K02192
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 08030:Family and consumer sciences, and culture and living-related
Research InstitutionTokyo University of Agriculture

Principal Investigator

AKUZAWA Sayuri  東京農業大学, 応用生物科学部, 教授 (60256641)

Co-Investigator(Kenkyū-buntansha) 日下部 裕子  国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門, ユニット長 (90353937)
花城 勲  鹿児島大学, 農水産獣医学域農学系, 准教授 (30336325)
早川 文代  国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門, ユニット長 (00282905)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords澱粉 / 味物質 / 構造解析 / レオロジー / 新規米 / 動的粘弾性 / 溶出糖 / 糖量 / アミロペクチン / 多糖類 / 拡散糖量 / 粘度 / 米澱粉 / 物性制御
Outline of Final Research Achievements

Starch synthase III-deficient rice mutant lines was generated using Tos17 insertion. We analyzed these rice grains, the starch structure, and the rheological properties of starch pastes. In addition, we evaluated the taste cell response using the developed taste DNA chip. This taste intensity was influenced by the molecular weight of these starches. This result showed that the perceptual intensity in taste cells can be quantified as same as the evaluation of taste intensity by sensory evaluation.

Academic Significance and Societal Importance of the Research Achievements

変異体米として、米に新たな機能性を付加することができる方法を示し、さらに、変異体米の米粒の炊飯特性、胚乳分離澱粉の構造とレオロジー特性、さらに味覚細胞を用いて、高分子多糖の物質特性により、共存する味物質の応答強度が異なることを示し、共存する「味物質」の知覚強度を評価する新しい味覚応答評価系を示した。変異体米は「米の機能性」と「味覚強度」の2要素を制御できる高次機能性食品素材として利用することができ、食品素材を用いた料理を考案することで、ヒトの摂食能力に応じた「味わう楽しみ」と「健康寿命の延伸」に貢献できると考える。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (24 results)

All 2020 2019 2018

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (15 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Relationships between the response of the sweet taste receptor, salivation toward sweeteners, and sweetness intensity2020

    • Author(s)
      Kusakabe Yuko、Shindo Yumiko、Kawai Takayuki、Maeda‐Yamamoto Mari、Wada Yuji
    • Journal Title

      Food Science & Nutrition

      Volume: 9 Issue: 2 Pages: 719-727

    • DOI

      10.1002/fsn3.2036

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of Taste Solutions with or without Aromas Based on the Relationship between Individual Resting and Stimulated Salivation2020

    • Author(s)
      Ogawa, Y., Kawai, T., Kusakabe, Y.
    • Journal Title

      Food Science and Technology Research

      Volume: 26 Issue: 3 Pages: 451-457

    • DOI

      10.3136/fstr.26.451

    • NAID

      130007882035

    • ISSN
      1344-6606, 1881-3984
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salivation Correlates with Masseter Muscle Activity and Partially Depends on the Physical Characteristics and Volume of Food Consumed at One Time2020

    • Author(s)
      Ogawa, Y., Kohyama, K., Kusakabe, Y.
    • Journal Title

      Food Science and Technology Research

      Volume: 26 Issue: 4 Pages: 535-543

    • DOI

      10.3136/fstr.26.535

    • NAID

      130007904091

    • ISSN
      1344-6606, 1881-3984
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of Cooked Rice and Starch Paste Properties of Mutant Rice2020

    • Author(s)
      Minemura, T., Matsumori, S., Akuzawa, S.
    • Journal Title

      Journal of Japan Health Medicine Association

      Volume: 29 Pages: 425-433

    • NAID

      130008033125

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential scanning fluorimetric analysis of the amino-acid binding to taste receptor using a model receptor protein, the ligand-binding domain of fish T1r2a/T1r32019

    • Author(s)
      Takashi Yoshida, Norihisa Yasui, Yuko Kusakabe, Chiaki Ito ,Miki Akamatsu, Atsuko Yamashita
    • Journal Title

      PLoS ONE

      Volume: 14 Issue: 10 Pages: e0218909-e0218909

    • DOI

      10.1371/journal.pone.0218909

    • NAID

      120006860091

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of fermented rice bran from Heshiko and isolation of amylase-producing bacteria2019

    • Author(s)
      J. Nagaoka, K. Wada, M. Katoh, N. Fujita, N. Tanaka, T. Irisawa, S. Akuzawa
    • Journal Title

      Food Preservation Science

      Volume: 45 Pages: 85-93

    • NAID

      40021857497

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 製糖時期の遅れが黒糖の品質に与える影響2019

    • Author(s)
      原博子,青山泰,阿久澤さゆり,横井琢也
    • Journal Title

      日本食品科学工学会誌

      Volume: 66 Pages: 9-17

    • NAID

      130007575107

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mental representation of domestic cooking operations among Japanese, consumers2018

    • Author(s)
      Y. Minami, Y. Kyutoko, M. Okamoto, Y. Kusakabe, T. Koizumi, I. Dan
    • Journal Title

      Int J Gastron Food Sci

      Volume: 13 Pages: 38-46

    • DOI

      10.1016/j.ijgfs.2018.05.005

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 静止唾液分泌量と刺激時唾液分泌量の関係に基づいた味と香りの評価2019

    • Author(s)
      日下部 裕子,河合 崇行,小川 雪乃
    • Organizer
      日本味と匂学会第53回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 水分散系に共存する多糖が甘味受容に及ぼす影響2019

    • Author(s)
      渡邉 敬、黒田華蓮、日下部裕子、阿久澤さゆり
    • Organizer
      日本食品科学工学会第66回
    • Related Report
      2019 Research-status Report
  • [Presentation] Sour suppressing effects by experienced flavors2019

    • Author(s)
      河合 崇行,日下部 裕子,小川 雪乃
    • Organizer
      The 18th International Symposium on Molecular and Neural Mechanisms of Taste and Olafactory Perception
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 甘味と連合記憶した香りによる酸味抑制効果2019

    • Author(s)
      河合 崇行,日下部 裕子,小川 雪乃
    • Organizer
      日本味と匂学会第53回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Evaluation of non-flavored/flavored taste solution using the relationship between resting and stimulated salivation2019

    • Author(s)
      小川 雪乃,神山 かおる,日下部 裕子
    • Organizer
      The 48th Naito Conference, Integrated Sensory Sciences - Pain, Itch, Smell and Taste
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 物理特性の異なるゲルの咀嚼による唾液分泌は咬筋活動と相関する2019

    • Author(s)
      小川 雪乃,神山 かおる,日下部 裕子
    • Organizer
      日本味と匂学会第53回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 澱粉の構造の解明に向けて2019

    • Author(s)
      花城勲
    • Organizer
      第59回 澱粉研究懇談会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 製麹における酵素発現様式と米のデンプン構造との関係2019

    • Author(s)
      峯田絵理、宮中宏暢、水上浩之、花城勲
    • Organizer
      日本食品科学工学会第66回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 剪断時間の異なる炊飯米ゲルの物理的特性と澱粉構造との関係2019

    • Author(s)
      峯田絵理、宮中宏暢、水上浩之、花城勲
    • Organizer
      日本応用糖質科学会令和元年度大会(第68回)
    • Related Report
      2019 Research-status Report
  • [Presentation] 味覚受容体T1R2/3の甘味物質応答に対する亜鉛イオンの作用2019

    • Author(s)
      喜多 志保子、日下部 裕子、山下 敦子
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 基本味刺激に対する唾液分泌量および唾液中成分の変化2018

    • Author(s)
      日下部 裕子、小川 雪乃、河合 崇行
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 食物の硬度・弾性・一回あたりの摂取量が唾液分泌量に及ぼす影響2018

    • Author(s)
      小川 雪乃、日下部 裕子
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Characterization of cooling agents by TRPM8 and TRPA1 activit.2018

    • Author(s)
      藤川誠二、宮澤利夫、佐久間克也、村西修一、日下部裕子
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Protein thermal shift assay indicated a broad amino acid-bindingcapability of the ligand-binding domains of fish T1r taste receptor2018

    • Author(s)
      吉田 高志、安井 典久、渥美 菜奈子、日下部 裕子、山下 敦子
    • Organizer
      The 17th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory perception
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of food hardness, elasticity, and amount taken at a time on salivation2018

    • Author(s)
      小川 雪乃、日下部 裕子
    • Organizer
      The 17th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory perception
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] メントールの刺激抑制剤および刺激抑制方法2018

    • Inventor(s)
      日下部 裕子 他7名
    • Industrial Property Rights Holder
      日下部 裕子 他7名
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Acquisition Date
      2018
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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