Controls of intestinal lipid metabolism by fish oil

• Project/Area Number: 18K05481
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: TAKAHASHI Nobuyuki 東京農業大学, 応用生物科学部, 教授 (50370135)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脂質代謝 / 魚油 / DHA / EPA / 消化管 / 食後高脂血症 / 炎症 / 血中中性脂肪濃度 / 脂肪酸酸化 / PPAR

Outline of Final Research Achievements

In this study, cellular and animal experiments were performed in order to elucidate a mechanism of amelioration in postprandial lipidemia. Oral lipid tolerance test (OLTT) revealed high-fat-diet and saturated fatty acids ameliorated the postprandial lipidemia. Expression levels of pro inflammatory cytokines were increased by the high-fat-diet and saturated fatty acids in the intestinal epithelia. On the other hand, differences in forms of DHA/EPA in fish oil could not be detected because of technical difficulties.

Academic Significance and Societal Importance of the Research Achievements

本研究において、魚油中のDHA・EPAの存在形態の違いによる抗炎症作用ならびに食後高脂血症に対する作用を明らかにすることはできなかった。しかし研究の過程において、高脂肪食摂取、特に飽和脂肪酸の摂取によって腸管上皮組織で炎症が誘導され、その腸管炎症が食後高脂血症を増悪化させる可能性が示唆された。この知見は、今後、食後高脂血症増悪化を改善する食品成分の同定・機能解析において、きわめて重要であり、食後高脂血症をターゲットとした機能性食品の開発につながることが期待される。

Research Products

• [Journal Article] A new mouse model for noninvasive fluorescence-based UCP1 expression monitoring. (2019)
  • Author(s): Kawarasaki S, Kuwata H, Sawazaki H, Sakamoto T, Nitta T, Kim CS, Jheng HF, Takahashi H, Nomura W, Ara T, Takahashi N, Tomita K Yu R, Kawada T, Goto T.
  • Journal Title: FEBS Letters Volume: 593 Pages: 1201-1212
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] beta-Cryptoxanthin induces UCP-1 expression via an RXR pathway in adipose tissue. (2019)
  • Author(s): Hara H, Takahashi H, Mohri S, Murakami H, Kawarasaki S, Iwase M, Takahashi N, Sugiura M, Goto T, Kawada T.
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 67 Pages: 10595-10603
  • Peer Reviewed
• [Journal Article] Food-derived compounds apigenin and luteolin modulate mRNA splicing of introns with weak splice sites. (2019)
  • Author(s): Kurata M, Fujiwara N, Fujita K, Yamanaka Y, Seno S, Kobayashi H, Miyamae Y, Takahashi N, Shibuya Y, Masuda S.
  • Journal Title: iScience Volume: 22 Pages: 336-352
  • NAID: 120007132694
  • Peer Reviewed
• [Presentation] AMPキナーゼの食後高脂血症改善作用への関与 (2020)
  • Author(s): 松田 真英、津山 博之、菊池 真優、田中 未央里、井上 博文、上原 万里子、高橋 信之
  • Organizer: 日本栄養・食糧学会