Analysis of genealogy and genome admixture of indigenous citrus varieties by statistical genetics analysis
| Project/Area Number |
18K05634
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39030:Horticultural science-related
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
SHIMIZU TOKUROU 国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, 上級研究員 (90355404)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 果樹 / カンキツ / 育種 / 系譜 / DNAマーカー / 多型 / ゲノム / バイオインフォマティクス / DNAマーカー分析 / ゲノム解析 / 栽培化 / 遺伝資源 / 遺伝子型 / 遺伝的多様性 / 親子関係 / 遺伝 / 品種分化 |
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| Outline of Final Research Achievements |
We confirmed citrus varieties' genetic identity, including Natsumikan, and found novel parent-child pedigrees from the comprehensive genotyping of more than 2,000 native citrus varieties. A survey of native Tachibana strains in Japan confirmed that individuals are maintained as clones in their native colony and then identified 11 Tachibana lines, including eight new lines. Based on the genetic similarity and geographical distribution of these Tachibana strains, we found that Tachibana emerged mainly in Miyazaki prefecture and confirmed that Tachibana-B is the Yamato Tachibana. To integrate the revealed pedigree for citrus breeding, we established a method for extracting genome-wide polymorphisms, proposed a novel method, "Citrus breeding 2.0" that avoids the limitations of conventional breeding, and launched a genome-wide polymorphism estimation method using GRAS-Di.
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| Academic Significance and Societal Importance of the Research Achievements |
遺伝子型をもとにカンキツの品種、系統の同一性を示すことで、品種異名の混乱を回避することが可能となった。また、従来一つとされてきたタチバナで11の系統が確認され、それらの発生地が宮崎県と推定されたことや、ヤマトタチバナを特定することができたことで、カンキツ研究に新ページを開いただけでなく、人文や歴史研究の深化にも貢献する。また、解明された系譜とゲノム情報を統合することで従来のカンキツ育種の制約の回避と効率化が可能となる。ゲノムワイド多型を利用して形質予測精度を向上する手法の開発にも着手し、カンキツを始めとする育種を効率化する。
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Markers, Maps, and Marker-Assisted Selection2020
Author(s)
Shimizu Tokurou、Aka Kacar Y?ld?z、Cristofani-Yaly Mari?ngela、Curtolo Maiara、Machado Marcos Antonio
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Journal Title
The Citrus Genome (Gentile A, La Malfa S and Deng Z eds.)
Volume: 7
Pages: 107-139
DOI
ISBN
9783030107994, 9783030153083
Related Report
Peer Reviewed / Int'l Joint Research
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