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A study on role of helper bacteria isolated from the fruiting body of ectomycorrhizal mushrooms, and their integrated utilization

Research Project

Project/Area Number 18K05764
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40020:Wood science-related
Research InstitutionTottori University

Principal Investigator

SHIMOMURA Norihiro  鳥取大学, 農学部, 教授 (00250093)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords外生菌根菌 / ショウロ / ヘルパー細菌
Outline of Final Research Achievements

Various bacterial strains were isolated from fruiting bodies of Rhizopogon roseolus. Some bacterial strains were found to stimulate mycelial growth in R. roseolus. BLAST analysis revealed that these bacterial strains belonged to the genera Paraburkholderia, Caballeronia, Janthinobacterium, Novosphingobium, and Rhodobacter. A comparative study using several ectomycorrhizal mushroom strains revealed that the bacterial strain from the fruiting body of R. roseolus stimulated mycelial growth of ectomycorrhizal mushrooms collected from a Pinus thunbergii forest, but not in those collected from another forest, indicating a specific interaction between the bacterial strain and ectomycorrhizal mushroom species. An in vitro inoculation experiment using P. thunbergii seedlings indicated that post-bacterial inoculation with GIB024 or GIB028 was effective in increasing mycorrhization of R. roseolus.

Academic Significance and Societal Importance of the Research Achievements

本研究で明らかにしたヘルパー細菌は,子実体から分離されていること,また,根圏土壌から分離されたヘルパー細菌とは種が異なることから,分離源の由来と細菌種に新規性がある.また,本ヘルパー細菌はクロマツを宿主としたきのこ種に対して特異的に菌糸生育を促進することを明らかにしているが,この特異性に関する新知見は,菌根性きのこと細菌の相互作用に関する新しい研究領域を提案することに寄与する.一方,ショウロ菌を効率的に宿主クロマツに感染させる有効な方法を提案したことから,本知見は菌根性きのこの高度利用に資するバイオ資材の開発にも貢献すると思われる.

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (37 results)

All 2021 2020 2019 2018

All Journal Article (15 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 14 results,  Open Access: 1 results) Presentation (21 results) (of which Int'l Joint Research: 12 results,  Invited: 6 results) Book (1 results)

  • [Journal Article] <b>Identification, characterization and expression of <i>A</i>-mating type genes in monokaryons and dikaryons of the edible mushroom <i>Mycoleptodonoides aitchisonii</i> (Bunaharitake)</b>2021

    • Author(s)
      Riffiani R., Chen F-C., Zhang W., Wada T., Shimomura N., Yamaguchi T. and Aimi T.
    • Journal Title

      Mycoscience

      Volume: 62 Issue: 2 Pages: 106-114

    • DOI

      10.47371/mycosci.2020.11.008

    • NAID

      130008000688

    • ISSN
      1340-3540, 1618-2545
    • Year and Date
      2021-03-20
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] エゾハリタケ子実体の安定形成条件の推定2021

    • Author(s)
      陳富杰・尾崎佑磨・會見忠則・霜村典宏
    • Journal Title

      日本きのこ学会誌

      Volume: 28 Pages: 182-186

    • NAID

      40022495161

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 奥深いキノコの世界―食用野生キノコの紹介と国内キノコ類栽培の現状と展望―2021

    • Author(s)
      早乙女梢・霜村典宏
    • Journal Title

      野菜情報

      Volume: 2 Pages: 2-5

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Spore formation and karyological characterization of basidiosporogenesis, meiosis, post-meiotic and nuclear migration mitosis in Coprinus comatus2020

    • Author(s)
      Chen F-C., Shimomura N., Yamaguchi T. and Aimi T.
    • Journal Title

      Mycoscience

      Volume: 61 Issue: 3 Pages: 122-127

    • DOI

      10.1016/j.myc.2020.02.001

    • NAID

      40022240626

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ササクレヒトヨタケ担子胞子の発芽促進と担子胞子分離技術の開発2020

    • Author(s)
      陳富嘉・陳富杰・尾崎佑磨・霜村典宏・山口武視・會見忠則
    • Journal Title

      日本きのこ学会誌

      Volume: 28 Pages: 62-67

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ササクレヒトヨタケの交配システム2020

    • Author(s)
      陳富嘉・陳富杰・早乙女梢・霜村典宏・山口武視・會見忠則
    • Journal Title

      日本きのこ学会誌

      Volume: 28 Pages: 93-99

    • NAID

      40022417444

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mating type in Mycoleptodonoides aitchisorii is not genetically associated with the monokaryotic clamp formation phenotype2020

    • Author(s)
      Riffiani, R., Wada, T., Chen, F-C., Shimomura, N., Yamaguchi, T. and Aimi, T.
    • Journal Title

      Mushroom Science and Biotechnology

      Volume: 27 Pages: 128-133

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation of bacteria from fruiting bodies of Rhizopogon roseolus and their effect on mycelial growth of host mushroom2020

    • Author(s)
      Pramoj Na Ayudhya, S., Riffiani, R., Ozaki, Y., Onda, Y., Nakano, S., Aimi, T and Shimomura, N.
    • Journal Title

      Mushroom Science and Biotechnology

      Volume: 27 Pages: 134-139

    • NAID

      130007981343

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mitochondrial DNA dynamics during fruiting body formation in Grifola frondosa2019

    • Author(s)
      Numata, F., Kawaguchi, N., Yamada, C., Ota, Y., Chen, F-C., Hayashi, M., Shimomura, N., Yamaguchi, T. and Aimi, T.
    • Journal Title

      Mycocience

      Volume: 60 Issue: 3 Pages: 147-150

    • DOI

      10.1016/j.myc.2019.01.003

    • NAID

      40021933268

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Monokariotic fruiting body and clamp cell formation in Mycoleptodonoides aitchisonii (Bunaharitake)2019

    • Author(s)
      Riffiani, R., Wada, T., Shimomura, N., Yamaguchi, T. and Aimi, T.
    • Journal Title

      Mycoscience

      Volume: 60 Issue: 3 Pages: 151-155

    • DOI

      10.1016/j.myc.2019.01.004

    • NAID

      40021933284

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic analyses of causal genes of albinism (white fruiting body) in Grifola frondosa.2019

    • Author(s)
      Kawaguchi, N., Hayashi, M., Chen, F.-C., Shimomura, N., Yamaguchi T. and Aimi, T.
    • Journal Title

      Journal of Wood Science

      Volume: 65: Issue: 1 Pages: 32-32

    • DOI

      10.1186/s10086-019-1811-7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of tyrosinase genes associated with fruiting body formation and pigmentation in Grifola frondosa2019

    • Author(s)
      Kawaguchi Nobuhisa、Hayashi Mirai、Nakano Shota、Shimomura Norihiro、Yamaguchi Takeshi、Aimi Tadanori
    • Journal Title

      Mycoscience

      Volume: 60 Issue: 4 Pages: 262-269

    • DOI

      10.1016/j.myc.2019.04.003

    • NAID

      40021955315

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of polyketide synthase gene 1 (pks1) in the fruiting body of Grifola frondosa post-harvest2019

    • Author(s)
      Kawaguchi, N., Hayashi, M., Shimomura, N., Yamaguchi, T. and Aimi, T
    • Journal Title

      Mushroom Science and Biotechnology

      Volume: 27 Pages: 67-69

    • NAID

      130007975787

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional analysis of ectomycorrhizas formed in Pinus thunbergii roots inoculated with Rhizopogon roseolus2019

    • Author(s)
      Pramoj Na Ayudhya, S., Sugimura, S., Ozaki, Y., Aimi, T. and Shimomura, N.
    • Journal Title

      Mushroom Science and Biotechnology

      Volume: 27 Pages: 93-97

    • NAID

      130007980109

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 外生菌根菌ショウロの人工栽培に関する研究2019

    • Author(s)
      霜村典宏
    • Journal Title

      日本きのこ学会誌

      Volume: 26 Pages: 148-155

    • NAID

      130007792886

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒラタケ子実体形成におけるオートファジー関連構造体のTEM観察による検出2019

    • Author(s)
      尾崎佑磨・會見忠則・霜村典宏
    • Organizer
      医学生物学電子顕微鏡技術学会 第35回学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] ヒラタケ子実体におけるオートファジー関連構造体の微細構造解析2019

    • Author(s)
      尾崎佑磨・會見忠則・霜村典宏
    • Organizer
      日本きのこ学会 第23回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] エゾハリタケ子実体の安定生産技術の開発2019

    • Author(s)
      陳富杰・會見忠則・霜村典宏
    • Organizer
      日本きのこ学会 第23回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] A glance of Faculty of Agriculture, Tottori University2019

    • Author(s)
      Shimomura, N.
    • Organizer
      The 3rd International Workshop Mushroom Biology and Technology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Detection of autophagy-related structure in the fruiting bodies of Pleurotus ostreatus2019

    • Author(s)
      Ozaki, Y., Aimi, T. and Shimomura, N.
    • Organizer
      Asian Mycology in Mie
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Isolation and characterization of bacteria from fruiting bodies of Rhizopogon roseolus2019

    • Author(s)
      Pramoj Na Ayudhya, S., Ozaki, Y., Onda, Y., Aimi, T. and Shimomura, N.
    • Organizer
      The 10th International Workshop on Edible Mycorrhizal Mushrooms
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mushroom use for enhanced salt-stress tolerance of host pine seedlings2019

    • Author(s)
      Shimomura, N.
    • Organizer
      International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2019 (AFELiSA2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Effects of isolated bacteria from Rhizopogon roseolus fruiting bodies on its mycelial growth and ectomycorrhizal formation2019

    • Author(s)
      Pramoj Na Ayudhya, S., Riffiari, R., Ozaki, Y., Aimi, T. and Shimomura, N.
    • Organizer
      International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2019 (AFELiSA2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Ultractructural aspect of autophagy-related structures in the fruiting body of Pleurotus ostreatus2019

    • Author(s)
      Ozaki, Y., Aimi, T. and Shimomura, N.
    • Organizer
      International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2019 (AFELiSA2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Culture medium improvement for the cell membrane stability (CMS) test of oyster mushroom (Pleurotus ostreatus) mycelia2019

    • Author(s)
      Miyatake, J., Suzuki, S., Hasanah, N. and Shimomura, N.
    • Organizer
      International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2019 (AFELiSA2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] UV-C irradiation for stimulation of mycelial growth of Rhizopogon roseolus.2019

    • Author(s)
      Yamada, T., Pramoj Na Ayudhya, S. and Shimomura, N.
    • Organizer
      International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2019 (AFELiSA2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] きのこ研究における電子顕微鏡の利用2019

    • Author(s)
      霜村典宏
    • Organizer
      第14回中四国電子顕微鏡研究会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] ポリリン酸を蓄積する海産珪藻Chaetoceros gracilisの生育に伴う微細構造変化2018

    • Author(s)
      安部大志・Hasanah, N.・星川淑子・霜村典宏
    • Organizer
      医学生物学電子顕微鏡技術学会 第34回学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] マイタケにおけるオートファジー関連構造体の透過型電子顕微鏡による検出2018

    • Author(s)
      尾崎佑磨・川口信久・會見忠則・霜村典宏
    • Organizer
      医学生物学電子顕微鏡技術学会 第34回学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] Breeding strategies on the edible ectomycorrhizal mushroom Rhizopogon roseolus: Strain improvement for enhanced salt-stress tolerance.2018

    • Author(s)
      Shimomura, N.
    • Organizer
      1st Symposium on Mushroom Research and Cultivation Technology: Progress & Challenges.
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] The role of specific mycorrhiza helper bacteria in the mycorrhization of Pinus thunbergii root in vitro2018

    • Author(s)
      Pramoj Na Ayudhya, S., Onda, Y., Aimi, T. and Shimomura, N.
    • Organizer
      日本きのこ学会 第22回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] イタケ子実体形成に伴う細胞内微細構造変化の解析2018

    • Author(s)
      尾崎佑磨・川口信久・會見忠則・霜村典宏
    • Organizer
      日本きのこ学会 第22回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 外生菌根菌ショウロの人工栽培に関する研究2018

    • Author(s)
      霜村典宏
    • Organizer
      日本きのこ学会 第22回大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Use of the ectomycorrhizal mushroom for revegetation of host pine trees under salt-stress condition2018

    • Author(s)
      Shimomura, N.
    • Organizer
      The International Symposium on Bioremediation, Revegetation, Biomaterial, and Conservation
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Ultrastructural analysis of intracellular structure during a fruiting body formation in Grifola frondosa2018

    • Author(s)
      Ozaki,Y., Kawaguchi, N., Aimi, T. and Shimomura, N.
    • Organizer
      International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2018 (AFELiSA2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of interaction between Rhizopogon roseolus and mycorrhiza helper bacteria on ectomycorrhizal formation in Pinus thunbergii roots in vitro2018

    • Author(s)
      Pramoj Na Ayudhya, S., Onda, Y., Aimi, T. and Shimomura, N.
    • Organizer
      International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2018 (AFELiSA2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] 外生菌根菌の人工栽培に関する取り組みと今後の展望 Functional Food―機能性食品の基礎から臨床へ―2019

    • Author(s)
      霜村典宏
    • Total Pages
      72
    • Publisher
      フジメディカル出版
    • ISBN
      9784862701855
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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