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Development of production method of high-optically pure lactic acid from non-sterilized biomass derived from rice manufacturing process by the arbitrary control of microbial flora in SSF.

Research Project

Project/Area Number 18K05920
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 41050:Environmental agriculture-related
Research InstitutionYamagata University

Principal Investigator

Watanabe Masanori  山形大学, 農学部, 教授 (20320020)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords光学活性乳酸 / 米加工副産物 / T-RFLP法 / バイオマスリファイナリー / 米由来バイオマス / 同時糖化発酵 / ポリ乳酸 / T-RFLP解析 / プロテアーゼ / バイオプラスチック / バクテリオシン / 微生物叢形成 / 微生物叢任意制御
Outline of Final Research Achievements

In this study, we evaluate the high-optically pure lactic acid production from non-sterilized biomass derived from rice by a newly isolated high-optically pure lactic acid producing bacterium (L. rhamnosus M23) under SSF cultivation. Antibacterial peptides were not produced in SSF culture. From the results of the T-RFLP analysis of SSF microbial flora, High lactic acid production was attained by the synergetic effect of decrease of dominance formation of Clostridium sp. cluster IX, XIVa and Bacteroidales, low accumulation of lower fatty acids and ethanol by the addition of acidic protease, pH control and oxygen partial supply SSF.

Academic Significance and Societal Importance of the Research Achievements

本課題は,SSF発酵液中のバイオマス表層構造,菌体外プロテアーゼ等酵素系の挙動及び機能,微生物群集構造などの複合的要因と乳酸生成能の関係を解明し,「米副産物を原料とした環境調和型高光学活性乳酸製造技術を確立する」点に学術的特徴がある。本研究の実施により,米由来バイオマスを非加熱・無殺菌状態のまま,乳酸製造の炭素源,栄養源,糖化酵素供給源として安定的に利用可能となれば,当該バイオマスの再資源化の促進のみならず,関連する廃棄物・廃水の減量化,資源・エネルギー循環社会形成等,産業化にも社会的にもその波及効果は極めて大きい。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (27 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (11 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 11 results,  Open Access: 5 results) Presentation (10 results) (of which Int'l Joint Research: 8 results,  Invited: 3 results) Remarks (3 results)

  • [Int'l Joint Research] Chiang Mai University(タイ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Chiang Mai University(タイ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Chiang Mai University(タイ)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Efficient Enzymatic Process for Mulberry Paper Production: An Approach for Xylooligosaccharide Production Coupled with Minimizing Bleaching Agent Doses2021

    • Author(s)
      Chaiyaso Thanongsak、Boonchuay Pinpanit、Takenaka Shinji、Techapun Charin、Rachtanapun Pornchai、Jantanasakulwong Kittisak、Watanabe Masanori
    • Journal Title

      Waste and Biomass Valorization

      Volume: open journal Issue: 10 Pages: 0-0

    • DOI

      10.1007/s12649-021-01416-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High frequency of transversion mutations in the rice (Oryza sativa L.) mutant population produced by diepoxy butane mutagenesis.2020

    • Author(s)
      KAWAKAMI, T., GOTO, H., ABE, Y., CHUBA, M., WATANABE, M., HOSHINO
    • Journal Title

      Genetic Resources and Crop Evolution

      Volume: 67 Issue: 6 Pages: 1355-1365

    • DOI

      10.1007/s10722-020-00950-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biochemical Characterization and Application of Thermostable-Alkaline Keratinase From Bacillus halodurans SW-X to Valorize Chicken Feather Wastes2020

    • Author(s)
      Kaewsalud Tanyawat、Yakul Kamon、Jantanasakulwong Kittisak、Tapingkae Wanaporn、Watanabe Masanori、Chaiyaso Thanongsak
    • Journal Title

      Waste and Biomass Valorization

      Volume: open journal Issue: 7 Pages: 0-0

    • DOI

      10.1007/s12649-020-01287-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 低真空窒素置換加熱法による新規米糠安定化技術の開発2020

    • Author(s)
      渡辺昌規、板垣泰士、阿部龍也、遠藤修二郎、星野友紀、西澤隆
    • Journal Title

      山形大学紀要(農学)

      Volume: 18 Pages: 11-19

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient bioconversion of enzymatic corncob hydrolysate into biomass and lipids by oleaginous yeast Rhodosporidium paludigenum KM2815102019

    • Author(s)
      Chaiyaso Thanongsak、Manowattana Atchara、Techapun Charin、Watanabe Masanori
    • Journal Title

      Preparative Biochemistry & Biotechnology

      Volume: 49 Issue: 6 Pages: 545-556

    • DOI

      10.1080/10826068.2019.1591985

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluating of quality of rice bran protein concentrate prepared by a combination of isoelectronic precipitation and electrolyzed water treatment2019

    • Author(s)
      Watanabe Masanori、Yamada Chikako、Maeda Isamu、Techapun Charin、Kuntiya Ampin、Leksawasdi Noppol、Seesuriyachan Phisit、Chaiyaso Thanongsak、Takenaka Shinji、Shiono Tadahiko、Nakamura Kozo、Endo Shujiro
    • Journal Title

      LWT

      Volume: 99 Pages: 262-267

    • DOI

      10.1016/j.lwt.2018.09.059

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Antihypertensive effects of orally administered eggplant (Solanum melongena) rich in acetylcholine on spontaneously hypertensive rats2019

    • Author(s)
      Yamaguchi Shohei、Matsumoto Kento、Koyama Masahiro、Tian Su、Watanabe Masanori、Takahashi Akihiko、Miyatake Koji、Nakamura Kozo
    • Journal Title

      Food Chemistry

      Volume: 276 Pages: 376-382

    • DOI

      10.1016/j.foodchem.2018.10.017

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nitrogen fixation in Rhodopseudomonas palustris co-cultured with Bacillus subtilis in the presence of air2019

    • Author(s)
      Haruka Arashida, Takumi Kugenuma, Masanori Watanabe, and Isamu Maeda
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 127 Issue: 5 Pages: 589-593

    • DOI

      10.1016/j.jbiosc.2018.10.010

    • NAID

      40021891617

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of thermostable alkaline protease from Bacillus halodurans SE5 and its application in degumming coupled with sericin hydrolysate production from yellow cocoon2019

    • Author(s)
      Yakul Kamon、Takenaka Shinji、Nakamura Kensuke、Techapun Charin、Leksawasdi Noppol、Seesuriyachan Phisit、Watanabe Masanori、Chaiyaso Thanongsak
    • Journal Title

      Process Biochemistry

      Volume: 78 Pages: 63-70

    • DOI

      10.1016/j.procbio.2019.01.003

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Direct bioconversion of rice residue from canteen waste into lipids by new amylolytic oleaginous yeast Sporidiobolus pararoseus KX7098722018

    • Author(s)
      Chaiyaso Thanongsak、Srisuwan Wimada、Techapun Charin、Watanabe Masanori、Takenaka Shinji
    • Journal Title

      Preparative Biochemistry and Biotechnology

      Volume: 48 Issue: 4 Pages: 361-371

    • DOI

      10.1080/10826068.2018.1446155

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] An integrated process for xylooligosaccharide and bioethanol production from corncob2018

    • Author(s)
      Boonchuay Pinpanit、Techapun Charin、Leksawasdi Noppol、Seesuriyachan Phisit、Hanmoungjai Prasert、Watanabe Masanori、Takenaka Shinji、Chaiyaso Thanongsak
    • Journal Title

      Bioresource Technology

      Volume: 256 Pages: 399-407

    • DOI

      10.1016/j.biortech.2018.02.004

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 環境調和型プロセスにより脱脂米糠から回収・精製された完全アレルゲンフリータンパク質及び機能性リン化合物の機能性食品原材料としての用途開発・市場導入に関する研究2021

    • Author(s)
      渡辺 昌規
    • Organizer
      日本農芸化学会(国内学会)
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Development of biomass refinery technology of rice manufacturing byproducts in Yamagata, Japan, for contribution to sustainable development goals.2020

    • Author(s)
      Masanori Watanabe, Chiako Yamada, Charin Techapun, Noppol Leksawasdi, Thanongsak Chaiyaso, Phisit Seesuriyachan, Shinji Takenaka
    • Organizer
      The international conference on food and applied bioscience 2020
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Physiological significance of high accumulation of phytic acid in rice bran and its relation with the taste and physical properties of rice.2020

    • Author(s)
      Kazuki Tada, Pilairuk Intipunya, Charin Techapun, Noppol Leksawasdi, Thanongsak Chaiyaso, Phisit Seesuriyachan, Shinji Takenaka, Masanori Watanabe.
    • Organizer
      The international conference on food and applied bioscience 2020
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Lipid production from defatted rice bran hydrolysate by using newly-isolated oleaginous yeasts.2020

    • Author(s)
      Kenta Kubota, Thanongsak Chaiyaso, Charin Techapun, Noppol Leksawasdi, Phisit Seesuriyachan, Shinji Takenaka, Masanori Watanabe.
    • Organizer
      The international conference on food and applied bioscience 2020
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Optimization of Thermostable alkaline keratinase production from Bacillus halodurance SW-X and its application on keratin hydrolysate production from chicken feather.2020

    • Author(s)
      Tanyawat Kaewsalud, Kamon Yakul, Charin Techapun, Masanori Watanabe, Thanongsak Chaiyaso.
    • Organizer
      The international conference on food and applied bioscience 2020
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Simultaneous recovery and purification of rice protein and phosphorus compounds from rice bran with environmentary harmonized process.2019

    • Author(s)
      Masanori Watanabe
    • Organizer
      1st International symposium on "Recent trend/technology of food security and management in Asia"
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 環境調和型プロセスにより脱脂米糠から回収・精製された完全アレルゲンフリータンパク質及び機能性リン化合物の機能性食品原材料としての用途開発・市場導入に関する研究2019

    • Author(s)
      渡辺昌規
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Characterization and mutation analysis of a halotolerant serine protease from Bacillus subtilis isolated from Thai traditional fermented shrimp pasete.2018

    • Author(s)
      Shinji Takenaka, Airi Takada, Charin Techapun, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso, Masanori Watanabe, Ampin Kuntiya
    • Organizer
      The final joint seminar of core to core program advanced research networks (2014-2019) on Establishment of an international research core for new bio-research fields with microbes from tropical areas
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characteristics of fermentive L-(+)-lactic acid production from non-sterilized by-product of rice by LAB's and its relationship with formation of microbial consortia in SSF2018

    • Author(s)
      Masanori Watanabe, Yuta Yamamura, Charin Techapun, Noppol Leksawasdi, Thanongsak Chaiyaso, Phisit Seesuriyachan, Ampin Kuntiya, Shinji Takenaka
    • Organizer
      The final joint seminar of core to core program advanced research networks (2014-2019) on Establishment of an international research core for new bio-research fields with microbes from tropical areas
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Purification, characterization of thermostable alkaline serine protease from Bacillus halodurans SE5 and its application on bio-bleaching of yellow cocoon.2018

    • Author(s)
      Thanongsak Chaiyaso, Kamon Yakul, Charin Techapun, Noppol Leksawasdi, Phisit Seesuriyachan, Masanori Watanabe, Kensuke Nakamura, Shinji Takenaka
    • Organizer
      The final joint seminar of core to core program advanced research networks (2014-2019) on Establishment of an international research core for new bio-research fields with microbes from tropical areas
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Remarks] 山形大学大学院農学研究科・渡辺研究室 バイオマス資源学研究室ホームページ

    • URL

      https://watanabelab.com

    • Related Report
      2020 Annual Research Report
  • [Remarks] 山形大学大学院農学研究科・渡辺研究室 バイオマス資源学研究室ホームページ

    • URL

      https://watanabelab.com/

    • Related Report
      2019 Research-status Report
  • [Remarks] 山形大学農学部・渡辺研究室 バイオマス資源学研究室ホームページ

    • URL

      https://watanabelab.com/

    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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