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Exosome-mediated intercellular crosstalk between endometrial cells and trophoblasts in cattle

Research Project

Project/Area Number 18K05989
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionIwate University

Principal Investigator

Kizaki Keiichiro  岩手大学, 農学部, 教授 (40337994)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsマイクロRNA / エクソソーム
Outline of Final Research Achievements

In the present study, we investigated on the microRNA (miRNA) contained in bovine endometrial cells and trophoblasts-derived exosomes, and attempted to elucidate the molecular mechanism of cell function by miRNA. As a result, some specific miRNAs from the endometrial cells and trophoblasts may bind to the 3'-untranslated and translated regions of pregnancy-related glycoprotein (PAG) and placenta lactogen (CSH2) mRNA. Inverse correlation of the expression kinetics was observed between trophoblast-specific mRNA and specific miRNA, suggesting that specific miRNA may regulate the expression of PAG and placenta lactogen protein. These results suggest that miRNA contributes to the interaction between endometrial cells and trophoblasts in implantation and placentation in cattle.

Academic Significance and Societal Importance of the Research Achievements

本研究では,子宮内膜細胞及び栄養膜細胞が産生するエクソソーム由来のmiRNAが相互の細胞に作用して、着床や胎盤形成時の情報伝達に関与する分子機構が明らかになる。即ち、エクソソームを介した細胞機能制御が着床・胎盤形成を確実にするというものであり、エクソソームを中心にした子宮内膜・栄養膜の細胞間クロストークの解明はウシの着床・胎盤形成機構の解明につながる。これらの結果は、牛の受胎性向上技術開発に資する知見であり、miRNAを利用した着床の促進あるいは避妊を調節する薬剤開発への展開を示唆するものである。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2022 2021 2020 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results)

  • [Journal Article] Analysis of circulating microRNA during early gestation in Japanese black cattle2022

    • Author(s)
      K.Ono, S.Okamoto, C.Ninomiya, N.Toji, T.Kanazawa, T.Ishiguro-Oonuma, T.Takahashi, K.Iga, K.Kizaki
    • Journal Title

      Domestic Animal Endocrinology

      Volume: 79 Pages: 106706-106706

    • DOI

      10.1016/j.domaniend.2021.106706

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウシ PLAC1 の発現分布2021

    • Author(s)
      渡邉佳和子,石黒(大沼)俊名,金澤朋美,高橋 透,伊賀浩輔,木崎景一郎
    • Organizer
      第114回日本繁殖生物学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 妊娠ウシ血中のマイクロRNA定量に関する基礎的検討II2020

    • Author(s)
      二宮千秋,木崎景一郎,石黒(大沼)俊名,金澤朋美,高橋 透,伊賀浩輔
    • Organizer
      第113回日本繁殖生物学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 妊娠ウシ血中のマイクロRNA定量に関する基礎的検討2019

    • Author(s)
      木崎景一郎,岡本さくら,石黒(大沼)俊名,高橋 透,伊賀浩輔
    • Organizer
      第112回日本繁殖生物学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ウシ妊娠子宮内膜および胎盤で発現するマイクロRNAの特性2019

    • Author(s)
      中津祥也,與座明祥,石黒(大沼)俊名,高橋 透,木崎景一郎
    • Organizer
      第162回日本獣医学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] miR-2328-3pのウシ子宮内膜・胎盤における標的遺伝子の探索2019

    • Author(s)
      中津祥也、與座明祥、石黒(大沼)俊名、高橋透、木崎 景一郎
    • Organizer
      日本畜産学会第125回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 妊娠ウシ血中マイクロRNAの網羅的解析2018

    • Author(s)
      岡本さくら、小野歌純、與座明祥、田路矩之、石黒(大沼)俊名、伊賀浩輔、木崎景一郎
    • Organizer
      第161回日本獣医学会学術集会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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