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Analysis of tissue- and development-specific gene function using a novel conditional-rescue transgenic mouse technique.

Research Project

Project/Area Number 18K06038
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42040:Laboratory animal science-related
Research InstitutionKitasato University

Principal Investigator

wtanabe daisuke  北里大学, 理学部, 講師 (00260175)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Discontinued (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsコンティショナルレスキュー / コンディショナルノックアウトマウス / Inv/ inversin / 左右軸形成 / 多発性嚢胞腎 / kit ligand / 幹細胞 / 疾患モデルマウス / コンティショナルレスキュー法 / inversin 遺伝子 / c-kit Sl 遺伝子 / disease model animal / transgenic rescue mice / conditional knockout / inversin / polycystic kidney / cilia / left/light asymmetry / c-kit / transgenic rescue / Inversin / transgenic mice / model animal
Outline of Final Research Achievements

Although the conditional knockout mouse method is effective for analyzing tissue-specific gene functions, it requires special techniques and a lot of time because ES cells are necessary for its generation. Therefore, we developed a conditional rescue technique (Patent No. 6327712) that does not require ES cells and is more convenient for analysis of gene function. In this study, in addition to the Inv gene, we generated novel conditional rescue mice target for the kit ligand genes, which play important roles in stem cell proliferation and migration, using a second-generation vector system that enables real-time observation of the switching of rescue gene expression. We have shown that these novel technologies are very effective for the analysis of the tissue- and development-specific gene function.

Academic Significance and Societal Importance of the Research Achievements

本研究においては新規のベクターを用いて、Inv遺伝子のほか新たにkit ligand遺伝子に対するコンディショナルレスキューマウスの作成に成功し、我々が開発した手法が多くの遺伝子の機能解析において有効的に機能することを明らかにした. 本手法は、従来の手法と比べ、短時間かつ低コストで目的とする遺伝子の組織または細胞特異的な欠失の誘導が可能となる新規の手法である.さらにこの手法は ES 細胞を必要としないため、ES 細胞が存在しないマウス系統やマウス以外の動物種にも適応可能である. 本手法は今後遺伝子の機能解析に広く適用されることが期待される.

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Research-status Report
  • 2018 Research-status Report

Research Products

(4 results)

All 2020 2019 2018

All Presentation (4 results)

  • [Presentation] Generation of novel conditional-rescue transgenic mice target for the functional ligand and its receptor gene.2020

    • Author(s)
      渡邉大介
    • Organizer
      日本分子生物学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] コンディショナルレスキュー法を用いた新規のトランスマウスの作成2020

    • Author(s)
      渡邉大介
    • Organizer
      文部科学省新学術領域研究 学術研究支援基盤形成 先端モデル動物支援プラットフォーム
    • Related Report
      2019 Research-status Report
  • [Presentation] Generation of the conditional-rescue transgenic mice that can regulate rescue-gene expression in the null mutant mice background.2019

    • Author(s)
      渡邉大介
    • Organizer
      日本分子生物学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Inv 遺伝子をターゲットとしたコンデショナルレスキューマウスの作製とその新技術の開発2018

    • Author(s)
      渡邉大介
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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