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Analysis of a novel transposon regulatory mechanism depending on DNA demethylation

Research Project

Project/Area Number 18K06050
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionHokkaido University

Principal Investigator

Ito Hidetaka  北海道大学, 理学研究院, 准教授 (70582295)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsトランスポゾン / エピジェネティクス / シロイヌナズナ / DNAメチル化 / 環境ストレス / 高温ストレス / ゲノム進化 / 環境適応 / サイレンシング / ヒストン修飾
Outline of Final Research Achievements

In Arabidopsis, a mutant of CMT3, a DNA methyltransferase responsible for the methylation of CHG sequences, reduced the expression of high-temperature activated transposons (TEs). This phenomenon suggests the existence of a regulatory mechanism that is different from the previously considered regulation of TEs by DNA methylation and repressive histone modifications. The transcriptional activation of TE is dependent on CMT3, and the loss-of-function mutation of CMT3 increases CHH methylation, which is mediated by CMT2. Furthermore, it was found that CMT3 inhibits the binding of CMT2 on the TE sequence, thereby suppressing CHH methylation and promoting TE transcription.

Academic Significance and Societal Importance of the Research Achievements

トランスポゾンの生存戦略の観点から、この高温活性型トランスポゾンはCMT3を利用して、CMT2が介在するCHHの過剰なメチル化を防ぎ、宿主植物による転写抑制から逃れている可能性がある。トランスポゾンの宿主ゲノムへの転移を伴うTEの生存は、厳しいストレス条件に対する植物の応答に新たな制御メカニズムを提供する可能性がある。今後、ストレス応答におけるこのトランスポゾンの影響を明らかにすることで、トランスポゾンに基づく制御メカニズムに光が当てられるだろう。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (3 results)

All 2019

All Presentation (3 results) (of which Invited: 1 results)

  • [Presentation] 利己的な遺伝子トランスポゾンと宿主植物の生存戦略2019

    • Author(s)
      伊藤 秀臣
    • Organizer
      北海道植物学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] A novel regulation of transposon expression via DNA methylation2019

    • Author(s)
      Masataka Yamada, Kosuke Nozawa, Atsushi Kato, Hidetaka Ito
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] A novel regulation of transposon via DNA methylation2019

    • Author(s)
      Masahiro Fukuda, Kosuke Nozawa, Kato Atsushi, Hidetaka Ito
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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