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Structural study on the mechanisms of the attachment of surface proteins and the formation of pili in a gram-positive bacteria Clostridium perfringens

Research Project

Project/Area Number 18K06087
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43020:Structural biochemistry-related
Research InstitutionKagawa University

Principal Investigator

Kamitori Shigehiro  香川大学, 医学部, 教授 (00262246)

Co-Investigator(Kenkyū-buntansha) 玉井 栄治  松山大学, 薬学部, 教授 (40333512)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsX線結晶構造解析 / 細菌細胞壁 / ソーテース / 線毛 / コンホメーション変化 / Piliタンパク質 / 酵素触媒反応機構 / ウェルシュ菌 / pilinタンパク質 / モデリング / Clostridium perfringens / 細胞壁 / 表面タンパク質
Outline of Final Research Achievements

Gram-positive bacteria possess a thick cell wall, a mesh polymer of peptidoglycans, that surrounds their cytoplasmic membranes, and many surface proteins are covalently attached to the cell wall. Pili, a type of surface proteins, are flexible rod proteins associated with the bacterial cell surface, that play important roles in the initial adhesion of bacterial cells to host tissues and their colonization. The pilus shaft is formed by covalent polymerization of major pilins, which is catalyzed by class C sortase, a family of cysteine transpeptidases. X-ray structures of major pilins from Clostridium perfringens strain 13 and SM101, and class C sortase from SM101 were determined, providing new insights into structural variation of pilin with dynamic conformational change and sortase-mediated formation of pilin.

Academic Significance and Societal Importance of the Research Achievements

本研究課題の成果により,病原性細菌ウェルシュ菌の宿主細胞への感染に関わる線毛はダイナミックなコンホメーション変化を起こしている可能性があること,線毛の重合・形成を触媒する酵素クラスCソーテースは活性部位とは別に基質結合サブサイトを持っていること等,学術的意義がある知見が得られた。ソーテースは,クラスにより様々な感染に関与する表面タンパク質を細胞壁に固定化することから,新たな抗菌薬のターゲットとしての可能性に注目が集まっている。これらの知見は,線毛およびソーテースをターゲットとした,感染防止薬・抗菌薬の開発に有用な情報を提供できる可能性がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2021 2020 2019 2018 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (6 results) Remarks (2 results)

  • [Journal Article] X-ray structures of Clostridium perfringens sortase C with C-terminal cell wall sorting motif of LPST demonstrate role of subsite for substrate-binding and structural variations of catalytic site2021

    • Author(s)
      Tamai Eiji、Sekiya Hiroshi、Nariya Hirofumi、Katayama Seiichi、Kamitori Shigehiro
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 554 Pages: 138-144

    • DOI

      10.1016/j.bbrc.2021.03.106

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural and biochemical characterizations of the novel autolysin Acd24020 from Clostridioides difficile and its full-function catalytic domain as a lytic enzyme.2021

    • Author(s)
      Sekiya, H., Tamai, E., Kawasaki, J., Murakami, K., Kamitori, S.
    • Journal Title

      Mol. Microbiol.

      Volume: 115 Issue: 4 Pages: 684-698

    • DOI

      10.1111/mmi.14636

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structures of major pilins in Clostridium perfringens demonstrate dynamic conformational change2019

    • Author(s)
      Tamai Eiji、Katayama Seiichi、Sekiya Hiroshi、Nariya Hirofumi、Kamitori Shigehiro
    • Journal Title

      Acta Crystallographica Section D Structural Biology

      Volume: 75 Issue: 8 Pages: 718-732

    • DOI

      10.1107/s2059798319009689

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ディフィシル菌オートライシンCD24020触媒ドメインのX線結晶解析に基づく基質認識機構の検討2020

    • Author(s)
      神鳥成弘,関谷洋志,玉井栄治
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ディフィシル菌のオートリシンCD24020 触媒ドメインの構造と機能解析2020

    • Author(s)
      玉井栄治,神鳥成弘,関谷洋志,河崎樹里奈,村上佳穂
    • Organizer
      第93回日本細菌学会総
    • Related Report
      2019 Research-status Report
  • [Presentation] ウェルシュ菌線毛タンパク質CppAとSortase CのX線結晶構造解析2019

    • Author(s)
      神鳥成弘,玉井栄治,関谷洋志,成谷宏文,片山誠一
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ウエルシュ菌線毛の構造解析2019

    • Author(s)
      玉井栄治,神鳥成弘,関谷洋志,片山誠一,成谷 宏文
    • Organizer
      第92回日本細菌学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] ウェルシュ菌細胞壁分解酵素CPE1138の生化学的解析とX線構造解析2019

    • Author(s)
      玉井 栄治,神鳥 成弘,成谷 宏文,関谷 洋志,渡部 あずさ,牧 純
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Research-status Report
  • [Presentation] X-ray structure of Clostridium perfringens CPE1138 catalytic domain hydrolyzing bacterial cell2018

    • Author(s)
      神鳥 成弘,玉井 栄治,関谷 洋志,牧 純,成谷 宏文
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Remarks] 科学研究費補助金報告書

    • URL

      http://www.med.kagawa-u.ac.jp/~xraylab/report/kaken/index.htm

    • Related Report
      2020 Annual Research Report
  • [Remarks] 科学研究費補助金(神鳥・吉田)

    • URL

      http://www.med.kagawa-u.ac.jp/~xraylab/report/kaken/index.htm

    • Related Report
      2019 Research-status Report 2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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