Elucidation of thermotolerant mechanism of the RNA essential for life activity under high-temperature environment
| Project/Area Number |
18K06088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43020:Structural biochemistry-related
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| Research Institution | The University of Tokushima (2020)
Ehime University (2018-2019) |
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Principal Investigator |
HIRATA Akira 徳島大学, 大学院社会産業理工学研究部(理工学域), 准教授 (60527381)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | tRNA modification / thermotolerant RNA / tRNA modification enzyme / Archaea / tRNA修飾 / アーキア / 耐熱化機構 / RNA成熟 / tRNAメチル化酵素 / 超好熱菌 / tRNA耐熱化機構 / tRNA修飾酵素 |
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| Outline of Final Research Achievements |
We determined the complete sequence of tRNATrp, one of the substrate tRNAs for archaeal Trm11 essential for m22G10 formation from Thermococcus kodakarensis (Tko), a hyperthermophilic archaeon. In Tko tRNATrp, we identified 15 types of modified nucleoside at 21 positions. Our genetic analysis suggests that trm11 gene is required for Tko survival at high temperatures over 95 ℃. We also demonstrated that trm14 gene is responsible for m2G67 formation. On the other hands, we suggest that Archaeosine synthase ArcS requires a racial SAM enzyme RaSEA for archaeosine synthesis at 15 position in the Tko tRNA.
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| Academic Significance and Societal Importance of the Research Achievements |
原始地球に生息した初期生命体は、高温環境下で生命活動を維持するために、修飾ヌクレオシドによりRNAを耐熱化する必要があったと考えられる。また、RNAの耐熱化に関わる修飾ヌクレオシドの合成経路を世界で初めて決定することができた。さらに、RNAを安定化する修飾ヌクレオシドも判明し、部位特異的にRNAを標識する技術開発やRNA創薬開発に新たな方向性を示すこととなった。今後、これらの研究成果を基盤に研究を継続することで、より発展的にRNAを安定化する革新的アイデアが生まれると期待している。
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Report
(4 results)
Research Products
(17 results)
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[Presentation] アーキア特異的修飾ヌクレオシド、アーケオシンの合成に関わる新奇ラジカルSAM酵素2019
Author(s)
横川隆志 , 能村友一朗 , 安田旭宏 , 尾木野弘実 , 日浦恵太 , 仲田沙織 , 岡 夏央 , 安藤香織 , 河村卓哉 , 平田 章 , 堀 弘幸 , 大野 敏
Organizer
第42回日本分子生物学会年会 福岡
Related Report
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[Presentation] Analysis of distinct modified nucleosides in tRNA from the hyperthermophilic archaeon Thermococcous kodakarensis provides insight into the requirement of specific tRNA modifications and their responsible genes for survival at high temperatures2019
Author(s)
Hirata, A , Suzuki, T , Nagano, T , Fuji, D , Okamoto, M , Sora, M , Lowe, M. T , Kanai, T , Atomi, H , Suzuki, T , Hori H
Organizer
Thermophiles2019 福岡
Related Report
Int'l Joint Research
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[Presentation] Structure of tRNA methyltransferase complex of Trm7 and Trm734 provides insight into its novel bipartite interaction essential for tRNA recognition and 2´-O-methylation at the first position of anticodon in specific tRNAs2019
Author(s)
Hirata, A , Okada, K , Yoshi, K , Shiraishi, H , Saijo, S , Yonezawa, K , Shimizu, N , Hori, H
Organizer
第21回日本RNA学会年会 東京大学伊藤国際学術研究センター
Related Report
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[Presentation] Structure of yeast tRNA Nm34 methyltransferase Trm7-Trm734 complex reveals its novel bipartite interaction essential for 2'-O-methylation of N34 in the wobble position of three specific tRNA species2018
Author(s)
Hirata, A., Okada, K., Yoshii, K., Shiraishi, H., Saijo, S., Yonezawa, K., Shimizu, N., and Hori, H.
Organizer
27th tRNA Conference
Related Report
Int'l Joint Research
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