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Integrated analysis of the bacterial divisome using multiple probes

Research Project

Project/Area Number 18K06094
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43020:Structural biochemistry-related
Research InstitutionRitsumeikan University

Principal Investigator

Matsumura Hiroyoshi  立命館大学, 生命科学部, 教授 (30324809)

Co-Investigator(Kenkyū-buntansha) 吉澤 拓也  立命館大学, 生命科学部, 助教 (50779056)
田中 俊一  京都府立大学, 生命環境科学研究科, 准教授 (70591387)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords細胞分裂タンパク質 / X線構造解析 / FtsZ / 細胞分裂 / Divisome / 阻害剤開発 / 蛍光プローブ開発 / 離合集散 / 細胞分裂装置 / マルチプローブ / 統合的解析
Outline of Final Research Achievements

FtsZ is a key protein in bacterial cell division and is assembled into filamentous architecture, which regulates bacterial cell division. FtsZ is also identified as an appealing new target for antibacterial agents. In this study, we developed a new fluorescent probe (BOFP) in which a BODIPY fluorophore has been conjugated to an oxazole-benzamide FtsZ inhibitor. Furthermore, development of high-speed AFM enables us to achieve sub-second time resolution and visualize the formation and dissociation process of FtsZ filaments. We also determined the crystal structures of the enzymatic domains of FtsZ from Klebsiella pneumoniae (KpFtsZ) and Escherichia coli (EcFtsZ) at 1.75 A and 2.50 A resolutions, respectively.

Academic Significance and Societal Importance of the Research Achievements

FtsZの収縮力の発生機構については、長年、様々なモデルが提唱されているが、決定的なものがまだない状況である。今回、EcFtsZの構造解析、および高速AFMによるFtsZの動的挙動解析を実施できたことで、その問いに迫るための準備が整った。また、新しくFtsZに特異的に結合する蛍光プローブが開発でき、初めて熱力学的パラメータを決定できたことは、今後のFtsZを標的とした創薬への重要な一歩である。今後、この蛍光プローブをFtsZの構造状態の同定に用いるといった応用も考えられ、学術面だけでなく新たな抗菌薬開発といった応用面へとつながる基盤が構築できたと考えている。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report

Research Products

(44 results)

All 2021 2020 2019 2018

All Journal Article (15 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 14 results,  Open Access: 7 results) Presentation (28 results) (of which Int'l Joint Research: 8 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Structure-function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis2021

    • Author(s)
      Kajiura Hiroyuki、Yoshizawa Takuya、Tokumoto Yuji、Suzuki Nobuaki、Takeno Shinya、Takeno Kanokwan Jumtee、Yamashita Takuya、Tanaka Shun-ichi、Kaneko Yoshinobu、Fujiyama Kazuhito、Matsumura Hiroyoshi、Nakazawa Yoshihisa
    • Journal Title

      Communications Biology

      Volume: 4 Pages: 1-13

    • DOI

      10.1038/s42003-021-01739-5

    • NAID

      120007147662

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanism of karyopherin-β2 binding and nuclear import of ALS variants FUS(P525L) and FUS(R495X)2021

    • Author(s)
      Gonzalez Abner、Mannen Taro、Cagatay Tolga、Fujiwara Ayano、Matsumura Hiroyoshi、Niesman Ashley B.、Brautigam Chad A.、Chook Yuh Min、Yoshizawa Takuya
    • Journal Title

      Scientific Reports

      Volume: 11 Pages: 1-15

    • DOI

      10.1038/s41598-021-83196-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy2021

    • Author(s)
      Fujita Junso、Sugiyama Shogo、Terakado Haruna、Miyazaki Maho、Ozawa Mayuki、Ueda Nanami、Kuroda Natsuko、Tanaka Shun-ichi、Yoshizawa Takuya、Uchihashi Takayuki、Matsumura Hiroyoshi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Pages: 1697-1697

    • DOI

      10.3390/ijms22041697

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insertion loop‐mediated folding propagation governs efficient maturation of hyperthermophilic Tk‐subtilisin at high temperatures2021

    • Author(s)
      Uehara R., Dan N., Amesaka H., Yoshizawa T., Koga Y.., Kanaya S., Takano K., Matsumura H., Tanaka SI.
    • Journal Title

      FEBS Letters

      Volume: 594 Pages: 452-461

    • DOI

      10.1002/1873-3468.14028

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Introduction of Sorghum RbcS with Rice RbcS Knockdown by RNAi on Photosynthetic Activity and Dry Weight in Rice.2021

    • Author(s)
      Kazuma Sakoda , Akito Yamamoto , Chie Ishikawa , Yojiro Taniguchi , Hiroyoshi Matsumura & Hiroshi Fukayama
    • Journal Title

      Plant Production Science

      Volume: in press Pages: 1-8

    • DOI

      10.1080/1343943x.2020.1847669

    • NAID

      120007125729

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4-Plant-like High Catalytic Activity2020

    • Author(s)
      Hiroyoshi Matsumura, Keita Shiomi, Akito Yamamoto, Yuri Taketani, Noriyuki Kobayashi, Takuya Yoshizawa, Shun-ichi Tanaka, Hiroki Yoshikawa, Masaki Endo, Hiroshi Fukayama,
    • Journal Title

      Molecular Plant

      Volume: 13 Pages: 1570-1581

    • DOI

      10.1016/j.molp.2020.08.012

    • NAID

      120006919994

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a GH1 β‐glucosidase from Hamamotoa singularis2020

    • Author(s)
      Uehara Ryo、Iwamoto Riki、Aoki Sayaka、Yoshizawa Takuya、Takano Kazufumi、Matsumura Hiroyoshi、Tanaka Shun‐ichi
    • Journal Title

      Protein Science

      Volume: 29 Pages: 2000-2008

    • DOI

      10.1002/pro.3916

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of nuclear import receptors on liquid–liquid phase separation2020

    • Author(s)
      Yoshizawa Takuya、Matsumura Hiroyoshi
    • Journal Title

      BIOPHYSICS

      Volume: 17 Issue: 0 Pages: 25-29

    • DOI

      10.2142/biophysico.bsj-2019052

      10.2142/biophysico.BSJ-2019052

    • NAID

      130007828794

    • ISSN
      2189-4779
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal structures of the cell-division protein FtsZ from Klebsiella pneumoniae and Escherichia coli2020

    • Author(s)
      Yoshizawa Takuya、Fujita Junso、Terakado Haruna、Ozawa Mayuki、Kuroda Natsuko、Tanaka Shun-ichi、Uehara Ryo、Matsumura Hiroyoshi
    • Journal Title

      Acta Crystallographica Section F Structural Biology Communications

      Volume: 76 Pages: 86-93

    • DOI

      10.1107/s2053230x2000076x

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structure-Guided Design of a Fluorescent Probe for the Visualization of FtsZ in Clinically Important Gram-Positive and Gram-Negative Bacterial Pathogens2019

    • Author(s)
      Ferrer-Gonzalez Edgar、Fujita Junso、Yoshizawa Takuya、Nelson Julia M.、Pilch Alyssa J.、Hillman Elani、Ozawa Mayuki、Kuroda Natsuko、Al-Tameemi Hassan M.、Boyd Jeffrey M.、LaVoie Edmond J.、Matsumura Hiroyoshi、Pilch Daniel S.
    • Journal Title

      Scientific Reports

      Volume: 9 Pages: 20092-20092

    • DOI

      10.1038/s41598-019-56557-x

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Affinity Improvement of a Cancer-Targeted Antibody through Alanine-Induced Adjustment of Antigen-Antibody Interface2019

    • Author(s)
      Yamashita Takefumi、Mizohata Eiichi、Nagatoishi Satoru、Watanabe Takahiro、Nakakido Makoto、Iwanari Hiroko、Mochizuki Yasuhiro、Nakayama Taisuke、Kado Yuji、Yokota Yuki、Matsumura Hiroyoshi、Kawamura Takeshi、Kodama Tatsuhiko、Hamakubo Takao、Inoue Tsuyoshi、Fujitani Hideaki、Tsumoto Kouhei
    • Journal Title

      Structure

      Volume: 27 Pages: 519-527

    • DOI

      10.1016/j.str.2018.11.002

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystallization of aspirin form II by femtosecond laser irradiation2018

    • Author(s)
      Tsuri Yuka、Maruyama Mihoko、Fujimoto Riki、Okada Shino、Adachi Hiroaki、Yoshikawa Hiroshi Y.、Takano Kazufumi、Murakami Satoshi、Matsumura Hiroyoshi、Inoue Tsuyoshi、Tsukamoto Katsuo、Imanishi Masayuki、Yoshimura Masashi、Mori Yusuke
    • Journal Title

      Appl. Phys. Express

      Volume: 12 Issue: 1 Pages: 015507-015507

    • DOI

      10.7567/1882-0786/aaf419

    • NAID

      210000135542

    • ISSN
      1882-0778, 1882-0786
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Growth of high-quality metastable crystal of acetaminophen using solution-mediated phase transformation at low supersaturation2018

    • Author(s)
      Fujimoto Riki、Maruyama Mihoko、Mori Yoichiro、Okada Shino、Adachi Hiroaki、Yoshikawa Hiroshi Y.、Takano Kazufumi、Murakami Satoshi、Matsumura Hiroyoshi、Inoue Tsuyoshi、Imanishi Masayuki、Tsukamoto Katsuo、Yoshimura Masashi、Mori Yusuke
    • Journal Title

      Journal of Crystal Growth

      Volume: 502 Pages: 76-82

    • DOI

      10.1016/j.jcrysgro.2018.09.012

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Atomic-Scale Imaging of Surface and Hydration Structures of Stable and Metastable Acetaminophen Crystals by Frequency Modulation Atomic Force Microscopy2018

    • Author(s)
      Kobayashi Naritaka、Maruyama Mihoko、Mori Yoichiro、Fukukita Suguru、Adachi Hiroaki、Takano Kazufumi、Murakami Satoshi、Matsumura Hiroyoshi、Inoue Tsuyoshi、Yoshimura Masashi、Nakabayashi Seiichiro、Mori Yusuke、Yoshikawa Hiroshi Y.
    • Journal Title

      Journal of Physical Chemistry C

      Volume: 122 Pages: 21983-21990

    • DOI

      10.1021/acs.jpcc.8b06928

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Initial, transient, and specific interaction between G protein-coupled receptor and target G protein in parallel signal processing: a case of olfactory discrimination of cancer-induced odors2018

    • Author(s)
      Sato Takaaki, Matsukawa Mutsumi, Mizutani Yoichi, Iijima Toshio, Matsumura Hiroyoshi
    • Journal Title

      Medical Research Archives

      Volume: 6 Pages: 1-1

    • Related Report
      2018 Research-status Report
  • [Presentation] C4光合成必須因子CP12-3およびその標的酵素GAPDHの構造機能解析2021

    • Author(s)
      松村浩由
    • Organizer
      新学術領域秋期領域会議
    • Related Report
      2020 Annual Research Report
  • [Presentation] Molecular basis of Karyopherin-β2 binding and nuclear import of ALS-related FUS2020

    • Author(s)
      Takuya Yoshizawa, Taro Mannen, Ayano Fujiwara, Hiroyoshi Matsumura
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Enzymatic activity of RNase H2 toward oxidized and abasic ribonucleoside monophophates embedded in DNA2020

    • Author(s)
      Yuki Ishizuka, Ryo Uehara, Takuya Yoshizawa, Hiroyoshi Matsumura
    • Organizer
      2020 World Conference on Protein Science
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Crystallographic studies of the cell-division protein FtsZ from Klebsiella pneumoniae and Escherichia coli2020

    • Author(s)
      Takuya Yoshizawa, Junso Fujita, Mayuki Ozawa, Natsuko Kuroda, Shun-ich Tanaka, Ryo Uehara, Hiroyoshi Matsumura
    • Organizer
      2020 World Conference on Protein Science
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PR poly-dipeptide blocks the chaperone activity of the Karyopherinβ2 for the Fused in sarcoma2020

    • Author(s)
      Takeru Uehara, Ayano Fujiwara, Ryo Uehara, Takuya Yoshizawa, Hiroyoshi Matsumura
    • Organizer
      2020 World Conference on Protein Science
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural analysis of FtsZ in complex with a fluorescent probe BOFP designed from binding structure of drug2020

    • Author(s)
      Natsuko Kuroda, Takuya Yoshizawa, Ryo Uehara, Junso Fujita, Mayuki Ozawa, Haruna Terakado, Daniel S. Pilch, Hiroyoshi Matsumura
    • Organizer
      2020 World Conference on Protein Science
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞分裂タンパク質FtsZに特異的に結合する蛍光プローブの開発2020

    • Author(s)
      黒田 奈津子、吉澤 拓也、上原 了、藤田 純三、小澤 真由季、寺門 榛名、Daniel S. Pilch、松村浩由
    • Organizer
      日本農芸化学会関西支部例会(第512回)
    • Related Report
      2019 Research-status Report
  • [Presentation] 毒性ポリペプチドによる相分離シャペロン阻害2019

    • Author(s)
      藤原彩乃、上原 武尊、上原 了、吉澤 拓也、松村 浩由
    • Organizer
      第4回LLPS研究会・ASUKA若手交流会2019
    • Related Report
      2019 Research-status Report
  • [Presentation] 光合成関連酵素の構造機能解析2019

    • Author(s)
      松村 浩由
    • Organizer
      新学術領域研究「新光合成」2019年度秋期領域会議
    • Related Report
      2019 Research-status Report
  • [Presentation] 30°Cおよび15°Cで栽培したホウレンソウRubiscoのX線構造解析2019

    • Author(s)
      西川 雄裕、上原 了、吉澤 拓也、松村 浩由
    • Organizer
      新学術領域研究「新光合成」2019年度秋期領域会議
    • Related Report
      2019 Research-status Report
  • [Presentation] 好熱性および常温性シアノバクテリアRubiscoの構造生物学的研究2019

    • Author(s)
      石原 拓弥、上原 了、吉澤 拓也、松村 浩由
    • Organizer
      新学術領域研究「新光合成」2019年度秋期領域会議
    • Related Report
      2019 Research-status Report
  • [Presentation] 炭酸固定酵素PEPCのフィードバック阻害脱感作機構の解明を目指した結晶学的研究2019

    • Author(s)
      堅川 舞陽、上原 了、吉澤 拓也、松村 浩由
    • Organizer
      新学術領域研究「新光合成」2019年度秋期領域会議
    • Related Report
      2019 Research-status Report
  • [Presentation] 植物光合成の高効率化に向けたカルビン回路タンパク質の構造機能解析2019

    • Author(s)
      篠原 航大、上原了、吉澤 拓也、松村 浩由
    • Organizer
      新学術領域研究「新光合成」2019年度秋期領域会議
    • Related Report
      2019 Research-status Report
  • [Presentation] Structural insight into the mechanism of Monobody-mediated alteration of enzyme substrate specificity2019

    • Author(s)
      Riki Iwamoto, Takuya Yoshizawa, Ryo Uehara, Kazufumi Takano, Shohei Koide, Hiroyoshi Matsumura, Shun-ichi Tanaka
    • Organizer
      JSBBA KANSAI 6th Student Forum
    • Related Report
      2019 Research-status Report
  • [Presentation] Structural analyses of the cell division protein FtsZ from E. coli and K. puemoniae2019

    • Author(s)
      Mayuki Ozawa, Haruna Terakado, Natsuko Kuroda, Ryo Uehara, Takuya Yoshizawa, Shun-ichi Tanaka, Hiroyoshi Matsumura
    • Organizer
      JSBBA KANSAI 6th Student Forum
    • Related Report
      2019 Research-status Report
  • [Presentation] Crystal structures of Rubisco purified from spinach grown at 30℃ and 15℃2019

    • Author(s)
      Takahiro Nishikawa, Ryo Uehara, Takuya Yoshizawa, Wataru Yamori, Hiroyoshi Matsumura
    • Organizer
      JSBBA KANSAI 6th Student Forum
    • Related Report
      2019 Research-status Report
  • [Presentation] Toxic poly-diptides target phase separation chaperone2019

    • Author(s)
      Ayano Fujiwara, Takeru Uehara, Ryo Uehara, Takuya Yoshizawa, Hiroyoshi Matumura
    • Organizer
      JSBBA KANSAI 6th Student Forum
    • Related Report
      2019 Research-status Report
  • [Presentation] Function and structure of multiheme-containing selenoprotein2019

    • Author(s)
      Ryuta Tobe, Takuya Yoshizawa, Yukiko Izu, Mst. Ishrat Jahan, Bunta Yoshimura, Hiroyoshi Matsumura, Hisaaki Mihara
    • Organizer
      第8回国際セレン学会
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] X-ray crystallographic analysis of S. aureus FtsZ complexed with inhibitors and a fluorescent probe2019

    • Author(s)
      Mayuki Ozawa, Junso Fujita, Takuya Yoshizawa, Natsuko Kuroda, Haruna Terakado, Ryo Uehara, Edgar Ferrer-Gonzalez, Julia M. Nelson, Alyssa J. Pilch, Elani Hillman, Ajit K. Parhi, Edmond J. LaVoie, Daniel S. Pilch, Hiroyoshi Matsumura
    • Organizer
      International Symposium on Diffraction Structure Biology 2019(ISDSB2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structural insight into the mechanism of Monobody-mediated alteration of enzyme substrate specificity2019

    • Author(s)
      Riki Iwamoto, Takuya Yoshizawa, Ryo Uehara, Kazufumi Takano, Shohei Koide, Hiroyoshi Matsumura, Shun-ichi Tanaka
    • Organizer
      International Symposium on Diffraction Structure Biology 2019(ISDSB2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] C4光合成を調節する新規分子の動的構造機能解析2019

    • Author(s)
      松村浩由
    • Organizer
      新学術領域研究「新光合成」2019年度春期領域会議
    • Related Report
      2019 Research-status Report
  • [Presentation] Dynamic structural analysis and drug design of S. aureus FtsZ2019

    • Author(s)
      Hiroyoshi Matsumura, Junso Fujita, Haruna Terakado, Mayuki Ozawa, Nanami Ueda, Shun-ich Tanaka, Takuya Yoshizawa
    • Organizer
      日本細菌学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] The 2nd Residue of GPCR Helix 8 May Control Transient and Specific Interaction with its G Protein2019

    • Author(s)
      Takaaki Sato, Hiroyoshi Matsumura
    • Organizer
      第9回アジア・オセアニア生理学会連合大会(FAOPS2019)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Engineering by Proxy: Monobody-mediated alteration of enzyme specificity2019

    • Author(s)
      Shunichi Tanaka, Tetsuya Takahashi, Akiko Koide, Riki Iwamoto, Hiroyoshi Matsumura, Takuya Yoshizawa, Kazufumi Takano, Satoshi Koikeda, and Shohei Koide
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] C4およびC3光合成調節タンパク質の動的構造機能解析2018

    • Author(s)
      松村浩由
    • Organizer
      新学術領域研究「新光合成」2018年度秋期領域会議
    • Related Report
      2018 Research-status Report
  • [Presentation] 細胞分裂必須タンパク質FtsZの機能と構造の研究2018

    • Author(s)
      寺門榛名、吉澤拓也、田中俊一、松村浩由
    • Organizer
      2018年度 関西四私大生命科学公開研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] Protein stability potentially governing protein evolution2018

    • Author(s)
      倉橋亮、田中俊一、佐野智、松村浩由、高野和文
    • Organizer
      Society for Molecular Biology and Evolution 2018
    • Related Report
      2018 Research-status Report
  • [Presentation] 光量変動と代謝調節をつなぐ新規分子の定量的手法を取り入れた構造機能解析2018

    • Author(s)
      松村浩由
    • Organizer
      新学術領域研究「新光合成」2018年度春期領域会議
    • Related Report
      2018 Research-status Report
  • [Book] 相分離生物学の全貌(現代化学増刊46)2020

    • Author(s)
      松村浩由、他
    • Total Pages
      416
    • Publisher
      東京化学同人
    • ISBN
      9784807913466
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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