Integrated analysis of the bacterial divisome using multiple probes
| Project/Area Number |
18K06094
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43020:Structural biochemistry-related
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉澤 拓也 立命館大学, 生命科学部, 助教 (50779056)
田中 俊一 京都府立大学, 生命環境科学研究科, 准教授 (70591387)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 細胞分裂タンパク質 / X線構造解析 / FtsZ / 細胞分裂 / Divisome / 阻害剤開発 / 蛍光プローブ開発 / 離合集散 / 細胞分裂装置 / マルチプローブ / 統合的解析 |
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| Outline of Final Research Achievements |
FtsZ is a key protein in bacterial cell division and is assembled into filamentous architecture, which regulates bacterial cell division. FtsZ is also identified as an appealing new target for antibacterial agents. In this study, we developed a new fluorescent probe (BOFP) in which a BODIPY fluorophore has been conjugated to an oxazole-benzamide FtsZ inhibitor. Furthermore, development of high-speed AFM enables us to achieve sub-second time resolution and visualize the formation and dissociation process of FtsZ filaments. We also determined the crystal structures of the enzymatic domains of FtsZ from Klebsiella pneumoniae (KpFtsZ) and Escherichia coli (EcFtsZ) at 1.75 A and 2.50 A resolutions, respectively.
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| Academic Significance and Societal Importance of the Research Achievements |
FtsZの収縮力の発生機構については、長年、様々なモデルが提唱されているが、決定的なものがまだない状況である。今回、EcFtsZの構造解析、および高速AFMによるFtsZの動的挙動解析を実施できたことで、その問いに迫るための準備が整った。また、新しくFtsZに特異的に結合する蛍光プローブが開発でき、初めて熱力学的パラメータを決定できたことは、今後のFtsZを標的とした創薬への重要な一歩である。今後、この蛍光プローブをFtsZの構造状態の同定に用いるといった応用も考えられ、学術面だけでなく新たな抗菌薬開発といった応用面へとつながる基盤が構築できたと考えている。
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4-Plant-like High Catalytic Activity2020
Author(s)
Hiroyoshi Matsumura, Keita Shiomi, Akito Yamamoto, Yuri Taketani, Noriyuki Kobayashi, Takuya Yoshizawa, Shun-ichi Tanaka, Hiroki Yoshikawa, Masaki Endo, Hiroshi Fukayama,
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Journal Title
Molecular Plant
Volume: 13
Pages: 1570-1581
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Crystallization of aspirin form II by femtosecond laser irradiation2018
Author(s)
Tsuri Yuka、Maruyama Mihoko、Fujimoto Riki、Okada Shino、Adachi Hiroaki、Yoshikawa Hiroshi Y.、Takano Kazufumi、Murakami Satoshi、Matsumura Hiroyoshi、Inoue Tsuyoshi、Tsukamoto Katsuo、Imanishi Masayuki、Yoshimura Masashi、Mori Yusuke
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Journal Title
Appl. Phys. Express
Volume: 12
Issue: 1
Pages: 015507-015507
DOI
NAID
ISSN
1882-0778, 1882-0786
Related Report
Peer Reviewed / Open Access
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[Presentation] X-ray crystallographic analysis of S. aureus FtsZ complexed with inhibitors and a fluorescent probe2019
Author(s)
Mayuki Ozawa, Junso Fujita, Takuya Yoshizawa, Natsuko Kuroda, Haruna Terakado, Ryo Uehara, Edgar Ferrer-Gonzalez, Julia M. Nelson, Alyssa J. Pilch, Elani Hillman, Ajit K. Parhi, Edmond J. LaVoie, Daniel S. Pilch, Hiroyoshi Matsumura
Organizer
International Symposium on Diffraction Structure Biology 2019（ISDSB2019）
Related Report
Int'l Joint Research
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