|Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|Outline of Final Research Achievements
The Intraflagellar transport (IFT)-B complex is involved in anterograde ciliary trafficking along the axoneme. Among 16 subunits of the IFT-B complex, IFT74-81 and IFT22-74-81 subcomplexes have been successfully expressed in E. coli using SUMO-tag and coexpression system. However, crystallization trials of the purified subcomplexes failed because of rapid protein degradation within a few days. By quick sample freezing after purification, a rod-like structure of IFT74-81 subcomplex was observed by cryo-EM single particle analysis.