| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Ion transport properties of various microbial rhodopsin were investigated under a high-time resolved condition by combining electrophysiology with a flash laser. Single turnover reaction of sodium ion transport by KR2, a sodium pumping rhodopsin was recorded. Kinetical analysis revealed that the turnover rate was 10 times faster than reported value obtained under multiple turnover conditions, suggesting two distinct reaction pathways in the KR2 photocycle.
Moreover, ion transport of 9 different proton pumping rhodopsins were systematically tested in conventional patch-clamp recording. The pumping activity of those are pH- and membrane-voltage dependent. Data revealed that the pH at the proton exclusion side largely affect the proton transport.
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