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Creaton of photo-activated ion channel for regulating intracellular ionic environment by light

Research Project

Project/Area Number 18K06168
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43040:Biophysics-related
Research InstitutionOkayama University (2022)
The Graduate School for the Creation of New Photonics Industries (2018-2021)

Principal Investigator

Hirano Minako  岡山大学, ヘルスシステム統合科学学域, 准教授 (80585167)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords光遺伝学 / イオンチャネル / オプトジェネティクス / 光感受性タンパク質 / 構造機能相関 / 光感受性蛋白質
Outline of Final Research Achievements

The aim of this study was to create a novel light-sensitive ion channel whose activity is controlled by non-invasive light to manipulate cellular functions by regulating the intracellular ionic environment with light. The structure-function relationships of the photoactivated adenylate cyclase (PAC) were investigated to identify the sites involved in photosensitivity, and light-dependent conformational changes of specific sites were also revealed. This information is expected to be useful for designing photosensitive chimeric channel mutants that combine PAC with potassium channels. Additionally, we established a system that combines a simple channel current measurement method with a light irradiation device, enabling the efficient examination of the light-dependent activity of these photosensitive chimeric channel mutants.

Academic Significance and Societal Importance of the Research Achievements

本研究により、新規光感受性イオンチャネルの構成要素であるPACの構造機能相関に関する情報が得られた。今後この情報をもとにカリウムチャネルとPACのキメラ変異体を設計することにより、光感受性が付与されたカリウムチャネルが創製できるであろう。すでにカリウムチャネルのイオン透過性をカルシウム透過性に改変することには成功しているので、光感受性カルシウムチャネルも創製できるはずである。これらの光感受性チャネルを用いれば、非侵襲な光で細胞内のイオン環境を変化することができるため、イオン環境に依存した疾患の原因解明や、究極的にはイオン環境の調節不全による疾患の治療のツールとしての展開が期待される。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2022 2021 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (7 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Development of an automated system to measure ion channel currents using a surface-modified gold probe.2021

    • Author(s)
      Minako Hirano, Masahisa Tomita, Chikako Takahashi, Nobuyuki Kawashima and Toru Ide
    • Journal Title

      Scientific reports

      Volume: 11(1) Issue: 1 Pages: 17934-17934

    • DOI

      10.1038/s41598-021-97237-z

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Lipid Bilayer Formed on a Hydrogel Bead for Single Ion Channel Recordings2020

    • Author(s)
      Hirano Minako、Yamamoto Daiki、Asakura Mami、Hayakawa Tohru、Mise Shintaro、Matsumoto Akinobu、Ide Toru
    • Journal Title

      Micromachines

      Volume: 11 Issue: 12 Pages: 1070-1070

    • DOI

      10.3390/mi11121070

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata2019

    • Author(s)
      Minako Hirano, Masumi Takebe, Tomoya Ishido, Toru Ide, Shigeru Matsunaga
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 20262-20262

    • DOI

      10.1038/s41598-019-56721-3

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Structural changes of adenylate cyclase from Oscillatoria acumminata in response to blue light stimulation2022

    • Author(s)
      北村有希、井出徹、平野美奈子
    • Organizer
      第60回 日本生物物理学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 光活性化アデニル酸シクラーゼの活性制御機構の理解に向けた研究・開発2022

    • Author(s)
      平野美奈子、建部益美、北村有希、三好佑奈、井出徹
    • Organizer
      第45回日本分子生物学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Development of an automated system for measuring channel currents using a gold probe2021

    • Author(s)
      平野 美奈子, 富田 正久, 髙橋 稚佳子, 川島 信幸, 井出 徹
    • Organizer
      第59回 日本生物物理学会
    • Related Report
      2021 Research-status Report
  • [Presentation] Development of a system for automated ionic current measurement2020

    • Author(s)
      平野 美奈子, 富田 正久, 髙橋 稚佳子, 川島 信幸, 井出 徹
    • Organizer
      第58回 日本生物物理学会
    • Related Report
      2020 Research-status Report
  • [Presentation] Identification of the activity-regulating site in the photoactivated adenylate cyclase (OaPAC)2019

    • Author(s)
      Minako Hirano, Tomoya Ishido, Masumi Takebe, Toru Ide, Shigeru Matsunaga
    • Organizer
      第57回 日本生物物理学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Light-dependent structural states of OaPAC2019

    • Author(s)
      Tomoya Ishido, Toru Ide, Minako Hirano
    • Organizer
      第57回 日本生物物理学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Modifications of K+ channel property2018

    • Author(s)
      Tomoya Ishido, Toru Ide, Minako Hirano
    • Organizer
      第56回 日本生物物理学会
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] 光活性化アデニル酸シクラーゼ2019

    • Inventor(s)
      平野 美奈子、松永 茂、建部 益美
    • Industrial Property Rights Holder
      浜松ホトニクス株式会社、学校法人光産業創成大学院大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-129374
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2024-01-30  

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