| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We previously developed in vitro enChIP, which is a biotechnology to isolate a target genomic region from cells. In this study, we combined in vitro enChIP with next-generation sequencing analysis (in vitro enChIP-Seq) to analyze B cell-specific chromosomal conformation. We focused on the Pax5 gene that encodes a master regulator of B cell-lineage commitment and succeeded in identifying genomic regions that interact commonly with the mouse and human Pax5 gene by in vitro enChIP-Seq. In addition, we analyzed histone marks on identified interacting genomic regions by ChIP-Seq and deleted those regions by genome editing to analyze physiological importance of the genomic interactions for Pax5 expression and B cell homeostasis.
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