Mechanism to drive the evolution of non self-recognizing loci controlling plant reproduction

Research Project

Project/Area Number	18K06178
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 43050:Genome biology-related
Research Institution	The University of Tokyo
Principal Investigator	Kubo Ken-ichi 東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (60403359)
Project Period (FY)	2018-04-01 – 2021-03-31
Project Status	Completed (Fiscal Year 2020)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2019: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000) Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords	自他識別 / 協調的非自己認識 / 自家不和合性 / 分子進化 / ゲノム生物学 / S-RNase型自家不和合性 / 植物ゲノム生物学
Outline of Final Research Achievements	Long-read sequencing was performed on the genomic DNA of Petunia (Solanaceae). We obtained accurate contigs containing more than 100 kb peripheral region of the alleles of the pistil-expressed S-RNase and the pollen-expressed multiple SLFs gene, which control the specificity of self-incompatibility. Comparison of peripheral regions of S-genes between different S-haplotypes revealed the evidences of large-scale genomic recombination and translocation between S loci. We suggest that the S locus has evolved through recombination between large genomic fragments to acquire SLF genes that are convenient for self and non-self discrimination.
Academic Significance and Societal Importance of the Research Achievements	自他識別特異性を維持するためには、雌ずい側因子と花粉側因子はユニットとして遺伝する必要がある。そのため、これまでS遺伝子座内では、組換えが抑制されていると考えられてきた。しかしながら、実際にはナス科のS遺伝子座はSハプロタイプ間での組換えを積極的に利用し、より効率的に非自己S-RNaseを解毒するSLFレパートリーを獲得し、受精の効率を最適化していることが判った。動物の獲得免疫にも似たメカニズムを、植物が集団レベルでどのように進化させてきたのか、理解が深まったと言える。植物がどのように生物多様性を持続させているのか、重要な知見を得ることができた。

Report

(4 results)

2020 Annual Research Report Final Research Report ( PDF )
2019 Research-status Report
2018 Research-status Report

Research Products
(1 results)

All Presentation (1 results) (of which Int'l Joint Research: 1 results, Invited: 1 results)

[Presentation] Non-self-recognition system in S-RNase-based self-incompatibility in Petunia: Molecular mechanism to avoid inbreeding.2018
- Author(s)
  久保健一
- Organizer
  第15回日本ナス科コンソーシアム年会
- Related Report
  2018 Research-status Report
- Int'l Joint Research / Invited