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Integrated analysis of the multi-layered tumor suppressor system woven by p53 and AMPK

Research Project

Project/Area Number 18K06206
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44010:Cell biology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Takafumi Miyamoto  筑波大学, 医学医療系, 助教 (50740346)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsp53 / AMPK / 細胞内構造 / オルガネラ
Outline of Final Research Achievements

Recently, metabolic regulation by the transcription factor p53 has been shown to play an important role in suppressing cancer. This p53-mediated metabolic regulation requires appropriate control of the spatiotemporal activity of AMPK, which is one of the target molecules for cancer therapy. However, the mechanism of cancer suppression by the p53-AMPK axis has not been fully elucidated.
Although this study did not elucidate the mechanism of cancer suppression by the p53-AMPK axis, we succeeded in developing fundamental technologies necessary for future research development, such as the relationship between p53 and nutrient metabolism and improved visualization of the spatiotemporal activity dynamics of AMPK.

Academic Significance and Societal Importance of the Research Achievements

転写因子p53やリン酸化酵素AMPKの機能を理解することは、より効果的ながんの治療法や予防法を開発していく上で、極めて有用である。
本研究ではp53-AMPK axisによるがん抑制メカニズムを解明するには至らなかったが、p53と栄養代謝の関連性、AMPKの時空間的な活性ダイナミクスの可視化の改良など、今後の研究展開に必要な基盤技術の開発に成功した。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report

Research Products

(13 results)

All 2020 2019 2018

All Journal Article (6 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Transcriptional co-repressor CtBP2 orchestrates epithelial-mesenchymal transition through a novel transcriptional holocomplex with OCT12020

    • Author(s)
      Ma Yang、Sekiya Motohiro、Kainoh Kenta、Matsuda Takaaki、Iwasaki Hitoshi、Osaki Yoshinori、Sugano Yoko、Suzuki Hiroaki、Takeuchi Yoshinori、Miyamoto Takafumi、Yahagi Naoya、Nakagawa Yoshimi、Matsuzaka Takashi、Shimano Hitoshi
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 523 Pages: 354-360

    • DOI

      10.1016/j.bbrc.2019.12.070

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Hepatocyte ELOVL Fatty Acid Elongase 6 Determines Ceramide Acyl-Chain Length and Hepatic Insulin Sensitivity in Mice2020

    • Author(s)
      Matsuzaka T, Kuba M, Koyasu S, Takeuchi Y, Yahagi N, Miyamoto T, Sekiya M, Nakagawa Y, Ema M, Takahashi S, Tokiwa H, Shimano H.
    • Journal Title

      Hepatology

      Volume: 71 Pages: 1609-1625

    • DOI

      10.1002/hep.30953

    • NAID

      120007132717

    • Description
      in press
    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ROCK 1 as a new player in the regulation of hepatic lipogenesis2019

    • Author(s)
      Miyamoto Takafumi、Matsuzaka Takashi、Shimano Hitoshi
    • Journal Title

      J Diabetes Investig.

      Volume: - Pages: 1165-1167

    • DOI

      10.1111/jdi.13051

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Octacosanol and policosanol prevent high-fat diet-induced obesity and metabolic disorders by activating brown adipose tissue and improving liver metabolism2019

    • Author(s)
      Sharma Rahu, Takafumi Miyamoto, et al.
    • Journal Title

      Sci Rep

      Volume: 9 Pages: 1-12

    • DOI

      10.1038/s41598-019-41631-1

    • NAID

      120007133465

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Glucocorticoid receptor suppresses gene expression of Rev-erbα (Nr1d1) through interaction with the CLOCK complex2019

    • Author(s)
      Murayama Yuki, Takafumi Miyamoto, et al.
    • Journal Title

      FEBS Lett

      Volume: 593 Pages: 423-432

    • DOI

      10.1002/1873-3468.13328

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cellular Application of Genetically Encoded Sensors and Impeders of AMPK2018

    • Author(s)
      Miyamoto Takafumi、Rho Elmer、Kim Allen、Inoue Takanari
    • Journal Title

      Methods Mol Biol

      Volume: - Pages: 255-272

    • DOI

      10.1007/978-1-4939-7598-3_17

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] NIS.aiを用いた非染色・非侵襲的な可視化技術の開発2020

    • Author(s)
      宮本崇史
    • Organizer
      第126回日本解剖学会総会・全国学術集会 第98回日本生理学会大会 合同大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 細胞機能を自在に制御する技術の開発2019

    • Author(s)
      宮本崇史
    • Organizer
      日本分子生物学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Argininosuccinate synthase 1 is an intrinsic Akt repressor transactivated by p532019

    • Author(s)
      Takafumi Miyamoto
    • Organizer
      Gordon Research Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 生物情報へのハッキングによる細胞操作技術の開発2019

    • Author(s)
      Takafumi Miyamoto
    • Organizer
      形態解析ワークショップ
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Hacking a living cell2018

    • Author(s)
      Takafumi Miyamoto
    • Organizer
      第10回 光塾
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] オルガネラの形態制御法iCOMの開発2018

    • Author(s)
      Takafumi Miyamoto
    • Organizer
      第27回日本バイオイメージング学会学術集会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] オルガネラの形態操作方法、オルガネラ形態変化用キット、オルガネラ形態操作のための細胞構成物質を使用した論理回路、およびアクチュエータータンパク質2019

    • Inventor(s)
      宮本崇史、松坂賢、島野仁
    • Industrial Property Rights Holder
      宮本崇史、松坂賢、島野仁
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-211266
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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