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Regulatory mechanism of the differentiation of multipotent stem cells in the tailbud and spinal cord development in vertebrate embryos.

Research Project

Project/Area Number 18K06242
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionSaitama University

Principal Investigator

Yamasu Kyo  埼玉大学, 理工学研究科, 教授 (60230439)

Co-Investigator(Kenkyū-buntansha) 津田 佐知子  埼玉大学, 理工学研究科, 准教授 (80736786)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsゼブラフィッシュ / 多分化能細胞 / 尾芽 / 体軸伸長 / 脊髄形成 / 神経発生 / Oct4型POU転写因子 / pou5f3 / 多分化能幹細胞 / 神経管形成 / 脊髄
Outline of Final Research Achievements

In this study, we investigated the involvement of pou5f3, the homologue of mouse Oct4, in body axis elongation in the teleost fish, zebrafish (Danio rerio). First, we showed that pou5f3 is expressed at the posterior end of the elongating neural tube. In addition, functional inhibition/activation and mutant analyses suggested that pou5f3 actually promotes elongation of the neural tube and activates neurogenic genes. It was further shown that pou5f3 expression in the tail bud is regulated by various secreted signals. These data show that pou5f3 is expressed under the control of various signals in the posterior neural tube and promotes neural development of immature cells migrating from the tail bud.

Academic Significance and Societal Importance of the Research Achievements

脊椎動物胚の体軸伸長が胚後端組織での組織新生によることは多くの動物種で知られており、様々な転写因子、分泌因子の関与が明らかになりつつある。一方で、POU型転写因子Oct4の哺乳類初期発生や多能性維持における重要性は広く知られる。本研究は、Oct4型遺伝子が脊髄の伸長を制御することを初めて明らかにしたものであり、学術的に重要な貢献と言える。
なお、ヒトの脊髄については二分脊椎、神経管閉鎖不全など、様々な先天性異常が知られる。事故などによる脊髄損傷の結果、麻痺などの後遺症が残ることもあり、日常生活に大きな支障をきたす。本研究の成果は、これらの疾患の発症機構理解や再生医療に貢献しうるものである。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (39 results)

All 2021 2020 2019 2018 Other

All Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 11 results,  Open Access: 4 results) Presentation (23 results) Book (2 results) Remarks (3 results)

  • [Journal Article] A globin-family protein, Cytoglobin 1, is involved in the development of neural crest-derived tissues and organs in zebrafish2021

    • Author(s)
      Takahashi Kazuki、Ito Yuki、Yoshimura Mami、Nikaido Masataka、Yuikawa Tatsuya、Kawamura Akinori、Tsuda Sachiko、Kage Daichi、Yamasu Kyo
    • Journal Title

      Developmental Biology

      Volume: 472 Pages: 1-17

    • DOI

      10.1016/j.ydbio.2020.12.016

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos2021

    • Author(s)
      Yuikawa Tatsuya、Ikeda Masaaki、Tsuda Sachiko、Saito Shinji、Yamasu Kyo
    • Journal Title

      Development, Growth & Differentiation

      Volume: 63 Issue: 6 Pages: 306-322

    • DOI

      10.1111/dgd.12742

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of somite patterning in the formation of Weberian apparatus and pleural rib in zebrafish.2020

    • Author(s)
      Akama, K., Ebata, K., Maeno, A., Taminato, T., Otosaka, S., Gengyo-Ando, K., Nakai, J., Yamasu, K., Kawamura, A.
    • Journal Title

      Journal of Anatomy

      Volume: 236 Issue: 4 Pages: 622-629

    • DOI

      10.1111/joa.13135

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of an Oct4-related PouV gene, pou5f3/pou2, in neurogenesis in the early neural plate of zebrafish embryos2020

    • Author(s)
      Inomata Chihiro、Yuikawa Tatsuya、Nakayama-Sadakiyo Yukiko、Kobayashi Kana、Ikeda Masaaki、Chiba Mizuki、Konishi Chihiro、Ishioka Akiko、Tsuda Sachiko、Yamasu Kyo
    • Journal Title

      Developmental Biology

      Volume: 457 Issue: 1 Pages: 30-42

    • DOI

      10.1016/j.ydbio.2019.09.002

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptional autoregulation of zebrafish tbx6 is required for somite segmentation2019

    • Author(s)
      Ban Hiroyuki、Yokota Daisuke、Otosaka Shiori、Kikuchi Morimichi、Kinoshita Hirofumi、Fujino Yuuri、Yabe Taijiro、Ovara Hiroki、Izuka Ayaka、Akama Kagari、Yamasu Kyo、Takada Shinji、Kawamura Akinori
    • Journal Title

      Development

      Volume: 146 Pages: 1-11

    • DOI

      10.1242/dev.177063

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 4D imaging identifies dynamic migration and the fate of gbx2-expressing cells in the brain primordium of zebrafish2019

    • Author(s)
      Tsuda Sachiko、Hiyoshi Kanae、Miyazawa Hiroaki、Kinno Risa、Yamasu Kyo
    • Journal Title

      Neuroscience Letters

      Volume: 690 Pages: 112-119

    • DOI

      10.1016/j.neulet.2018.09.027

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Deadenylation by the CCR4-NOT complex contributes to the turnover of hairy-related mRNAs in the zebrafish segmentation clock.2018

    • Author(s)
      Fujino Y, Yamada K, Sugaya C, Ooka Y, Ovara H, Ban H, Akama K, Otosaka S, Kinoshita H, Yamasu K, Mishima Y, Kawamura A.
    • Journal Title

      FEBS letters

      Volume: 592(20) Issue: 20 Pages: 3388-3398

    • DOI

      10.1002/1873-3468.13261

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Optical measurement of neuronal activity in the developing cerebellum of zebrafish using voltage-sensitive dye imaging2018

    • Author(s)
      Okumura Kanoko、Kakinuma Hisaya、Amo Ryunosuke、Okamoto Hitoshi、Yamasu Kyo、Tsuda Sachiko
    • Journal Title

      NeuroReport

      Volume: 29 Issue: 16 Pages: 1349-1354

    • DOI

      10.1097/wnr.0000000000001113

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Functional roles of the Ripply-mediated suppression of segmentation gene expression at the anterior presomitic mesoderm in zebrafish2018

    • Author(s)
      Kinoshita Hirofumi、Ohgane Nanae、Fujino Yuuri、Yabe Taijiro、Ovara Hiroki、Yokota Daisuke、Izuka Ayaka、Kage Daichi、Yamasu Kyo、Takada Shinji、Kawamura Akinori
    • Journal Title

      Mechanisms of Development

      Volume: 152 Pages: 21-31

    • DOI

      10.1016/j.mod.2018.06.001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Optical interrogation of neuronal circuitry in zebrafish using genetically encoded voltage indicators2018

    • Author(s)
      Miyazawa Hiroaki、Okumura Kanoko、Hiyoshi Kanae、Maruyama Kazuhiro、Kakinuma Hisaya、Amo Ryunosuke、Okamoto Hitoshi、Yamasu Kyo、Tsuda Sachiko
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 6048-6048

    • DOI

      10.1038/s41598-018-23906-1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a ( chata ) gene2018

    • Author(s)
      Nikaido Masataka、Izumi Saki、Ohnuki Honoka、Takigawa Yuki、Yamasu Kyo、Hatta Kohei
    • Journal Title

      Gene Expression Patterns

      Volume: 28 Pages: 12-21

    • DOI

      10.1016/j.gep.2018.01.003

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Genetic analysis of the mechanism involved in dorsoventral patterning of the developing telencephalon in zebrafish embryos2021

    • Author(s)
      Koto Umeda, Takuma Kamimura, Masato Maekawa, Shiho Suzuki, Kaiho Tanaka, Kyo Yamasu
    • Organizer
      27th Japanese Medaka Zebrafish Meeting
    • Related Report
      2021 Annual Research Report
  • [Presentation] Regulation of neurogenesis by pou5f3 and retinoic acid in the caudal region of the extending neural tube in zebrafish embryos2021

    • Author(s)
      結川 達也 , 安田 楓 , 石倉 愛悠 , 弥益 恭
    • Organizer
      第44回 日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Developmental genetics study of telencephalon dorsal-ventral patterning in zebrafish2021

    • Author(s)
      上村 拓真、梅田 琴、前川 雅人、鈴木 志保、田中 海帆、弥益 恭
    • Organizer
      第44回 日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Development of the feedback subloop mechanism involved in the biological clock of the zebrafish2021

    • Author(s)
      大﨑 翔梧、池田 亮、今川 夏音;、弥益恭
    • Organizer
      第44回 日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 伸長中の神経管後端における神経発生のpou5f3とBMPシグナルによる制御2020

    • Author(s)
      結川達也、佐藤武寿、津田佐知子、弥益 恭
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 脊椎動物胚における峡部オーガナイザー細胞の発生運命追跡の試み2020

    • Author(s)
      大柳 毅朗、鈴木志保、弥益 恭
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Fgf8発現細胞の発生過程での挙動を忠実に再現する蛍光標識ゼブラフィッシュ系統の作製2020

    • Author(s)
      鈴木 志保,弥益 恭
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Regulation of the posterior development in zebrafish embryos by FGF and Wnt signaling.2020

    • Author(s)
      Yuikawa T, Ikeda M, Tsuda S and Yamasu K
    • Organizer
      26th Japanese Medaka Zebrafish Meeting
    • Related Report
      2020 Research-status Report
  • [Presentation] 峡部オーガナイザーにおける神経発生制御機構と峡部細胞の発生運命の追跡2020

    • Author(s)
      大柳 毅朗、鈴木 志保、弥益 恭
    • Organizer
      第43回 日本分子生物学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 発生過程におけるfgf8a発現細胞のリアルタイムイメージングと追跡を可能にするゼブラフィッシュ系統の作製2020

    • Author(s)
      鈴木 志保、大柳 毅朗、津田 佐知子、弥益 恭
    • Organizer
      第43回 日本分子生物学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] Involvement of pou5f3 and soxB1 in neurogenesis at the caudal region of the extending body axis was shown using zebrafish mutants.2019

    • Author(s)
      Tatsuya Yuikawa , Takehisa Sato , Sachiko Tsuda , Kyo Yamasu
    • Organizer
      第42回 日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Evaluation of the role of the shadow enhancers for gbx2 in zebrafish using genome editing techniques.2019

    • Author(s)
      Sota Saito, Kyo Yamasu
    • Organizer
      第42回 日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Neurogenesis in the caudal region of the extending body axis in zebrafish embryos is regulated by pou5f3 and soxB1genes.2019

    • Author(s)
      Tatsuya Yuikawa, Takehisa Sato, Sachiko Tsuda, Kyo Yamasu
    • Organizer
      25th Japanese Medaka Zebrafish Meeting
    • Related Report
      2019 Research-status Report
  • [Presentation] Studies on the roles of the shadow enhancers of zebrafish gbx2 during development using genome editing approaches.2019

    • Author(s)
      Sota Saito, Kyo Yamasu
    • Organizer
      25th Japanese Medaka Zebrafish Meeting
    • Related Report
      2019 Research-status Report
  • [Presentation] Takero Ohyanagi, Shiho Suzuki, Kyo Yamasu2019

    • Author(s)
      Studies on neurogenesis in the isthmic organizer region and its later development.
    • Organizer
      25th Japanese Medaka Zebrafish Meeting
    • Related Report
      2019 Research-status Report
  • [Presentation] Shiho Suzuki, Kyo Yamasu2019

    • Author(s)
      Fluorescence labeling of brain forming genes by the homologous recombination-independent knock-in technology.
    • Organizer
      25th Japanese Medaka Zebrafish Meeting
    • Related Report
      2019 Research-status Report
  • [Presentation] The roles of the transcriptional repressive region of zebrafish fgf8a in development revealed by the CRISPR/Cas9 system.2018

    • Author(s)
      Saito S and Yamasu K
    • Organizer
      第41回 日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Extension of the posterior structure in zebrafish embryos is regulated by FGF and Wnt signaling.2018

    • Author(s)
      Yuikawa T, Sato S, Ikeda M, Tsuda S and Yamasu K
    • Organizer
      第41回 日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Functional compartmentalization of the cerebellar circuits: three-dimensional analysis in zebrafish2018

    • Author(s)
      Hiyoshi K, Yamasu K and Tsuda S
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Optical measurement of neuronal activity in zebrafish brain by genetically encoded voltage indicators2018

    • Author(s)
      Okumura K, Miyazawa H, Hiyoshi K, Maruyama K, Kakinuma H, Amo R, Okamoto H, Yamasu K and Tsuda S
    • Organizer
      第70回日本細胞生物学会/第51回日本発生生物学会合同大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Deletion of the transcriptional regulatory region of zebrafish fgf8a affects development of the muscle and notochord.2018

    • Author(s)
      Saito S and Yamasu K
    • Organizer
      24th Japanese Medaka Zebrafish Meeting
    • Related Report
      2018 Research-status Report
  • [Presentation] Regulation of the posterior development in zebrafish embryos by FGF and Wnt signaling.2018

    • Author(s)
      Yuikawa T, Ikeda M, Tsuda S and Yamasu K
    • Organizer
      24th Japanese Medaka Zebrafish Meeting
    • Related Report
      2018 Research-status Report
  • [Presentation] Zebrafish pou5f3, an Oct4-type class-V POU gene, is involved in neurogenesis in the caudal neural tube.2018

    • Author(s)
      Yuikawa T, Ikeda M, Tsuda S and Yamasu K
    • Organizer
      第70回日本細胞生物学会・第51回日本発生生物学会合同年会
    • Related Report
      2018 Research-status Report
  • [Book] 発生生物学―基礎から応用への展開2019

    • Author(s)
      塩尻 信義, 弥益 恭, 加藤 容子, 中尾 啓子(共編)
    • Total Pages
      169
    • Publisher
      培風館
    • ISBN
      9784563078232
    • Related Report
      2019 Research-status Report
  • [Book] 新しい生物科学2018

    • Author(s)
      弥益 恭、中尾啓子、野口 航(編)
    • Total Pages
      193
    • Publisher
      培風館
    • Related Report
      2018 Research-status Report
  • [Remarks] 埼玉大学大学院理工学研究科生命科学部門 理学部生体制御学科 発生生物学研究室

    • URL

      http://devbiol.seitai.saitama-u.ac.jp

    • Related Report
      2021 Annual Research Report
  • [Remarks] 発生生物学研究室

    • URL

      http://devbiol.seitai.saitama-u.ac.jp

    • Related Report
      2020 Research-status Report
  • [Remarks] 埼玉大学発生生物学研究室

    • URL

      http://devbiol.seitai.saitama-u.ac.jp

    • Related Report
      2019 Research-status Report 2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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