Interaction between nuclear pore complex and chromatin in plants

• Project/Area Number: 18K06283
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44030:Plant molecular biology and physiology-related
• Research Institution: University of Shizuoka
• Principal Investigator: Tamura Kentaro 静岡県立大学, 食品栄養科学部, 准教授 (40378609)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: シロイヌナズナ / 核膜 / 核膜孔 / 遺伝子発現 / ヌクレオポリン / 核ラミナ / クロマチン / 遺伝子発現制御 / 花粉 / サリチル酸 / 病害応答 / クロマチン構造 / 植物免疫

Outline of Final Research Achievements

The nuclear pore complex is a multi-functional channel regulating nucleo-cytoplasmic transport in eukaryotes. The nuclear pore complex is composed of more than 30 nucleoporin proteins in higher plants. In this study, we have revealed interaction between nucleoporin and specific chromatin regions in Arabidopsis and that this interaction depends on salicylic acid signaling. We also established in-vitro assay system of nuclear envelop reconstitution with extracts from plant cultured cells. This system might provide important insights into the molecular machinery of the nuclear envelop interaction with chromatin in plants.

Academic Significance and Societal Importance of the Research Achievements

核膜孔複合体および核膜は真核生物に広く保存された構造であるが，その機能は多岐にわたることが推定されていた．私達がこれまでに見いだしていた植物独自の核膜孔や核膜成分の解析をベースとして研究を展開した．本研究成果では，植物が進化の過程で独自に手に入れた遺伝子発現制御機構および，器官発生における核膜成分の新しい役割と機能を明らかにすることができた．これらの成果は効率的な食料増産を進めるうえで重要な分子基盤となることが期待される．

Research Products

• [Int'l Joint Research] INRA/IBMP(フランス)
• [Int'l Joint Research] INRA/IJPB(フランス)
• [Int'l Joint Research] University of Clermont Auvergne/IBMP(フランス)
• [Journal Article] Validation of nuclear pore complex protein-protein interactions by transient expression in plants (2022)
  • Author(s): Fumika Ikeda、Kentaro Tamura
  • Journal Title: Methods in Molecular Biology Volume: 2502 Pages: 235-243
  • DOI: 10.1007/978-1-0716-2337-4_15
  • ISBN: 9781071623367, 9781071623374
  • Peer Reviewed / Open Access
• [Journal Article] Spatiotemporal relationship between auxin dynamics and hydathode development in Arabidopsis leaf teeth (2021)
  • Author(s): Yagi Hiroki、Tamura Kentaro、Matsushita Tomonao、Shimada Tomoo
  • Journal Title: Plant Signaling and Behavior Volume: 16 Issue: 12 Pages: 1989216-1989216
  • DOI: 10.1080/15592324.2021.1989216
  • Peer Reviewed / Open Access
• [Journal Article] Regulation and Physiological Significance of the Nuclear Shape in Plants (2021)
  • Author(s): Goto Chieko、Hara-Nishimura Ikuko、Tamura Kentaro
  • Journal Title: Frontiers in Plant Science Volume: 12 Pages: 67390-67390
  • DOI: 10.3389/fpls.2021.673905
  • Peer Reviewed / Open Access
• [Journal Article] In vitro assembly of nuclear envelope in tobacco cultured cells (2021)
  • Author(s): Tamura Kentaro、Ueda Haruko、Hara-Nishimura Ikuko
  • Journal Title: Nucleus Volume: 12 Issue: 1 Pages: 82-89
  • DOI: 10.1080/19491034.2021.1930681
  • Peer Reviewed / Open Access
• [Journal Article] Fluorescent protein-based imaging and tissue-specific RNA-seq analysis of Arabidopsis hydathodes (2020)
  • Author(s): Yagi Hiroki、Nagano Atsushi J、Kim Jaewook、Tamura Kentaro、Mochizuki Nobuyoshi、Nagatani Akira、Matsushita Tomonao、Shimada Tomoo
  • Journal Title: Journal of Experimental Botany Volume: 72 Issue: 4 Pages: 1260-1270
  • DOI: 10.1093/jxb/eraa519
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural and functional relationships between plasmodesmata and plant endoplasmic reticulum?plasma membrane contact sites consisting of three synaptotagmins (2020)
  • Author(s): Ishikawa Kazuya、Tamura Kentaro、Fukao Yoichiro、Shimada Tomoo
  • Journal Title: New Phytologist Volume: 226 Issue: 3 Pages: 798-808
  • DOI: 10.1111/nph.16391
  • Peer Reviewed / Open Access
• [Journal Article] Nuclear pore complex-mediated gene expression in Arabidopsis thaliana (2020)
  • Author(s): Tamura Kentaro
  • Journal Title: Journal of Plant Research Volume: 133 Issue: 4 Pages: 449-455
  • DOI: 10.1007/s10265-020-01177-0
  • Peer Reviewed
• [Journal Article] The nuclear envelope protein KAKU4 determines the migration order of the vegetative nucleus and sperm cells in pollen tubes (2020)
  • Author(s): Goto Chieko、Tamura Kentaro、Nishimaki Satsuki、Maruyama Daisuke、Hara-Nishimura Ikuko
  • Journal Title: Journal of Experimental Botany Volume: 71 Issue: 20 Pages: 6273-6281
  • DOI: 10.1093/jxb/eraa367
  • Peer Reviewed / Open Access
• [Journal Article] Subnuclear gene positioning through lamina association affects copper tolerance (2020)
  • Author(s): Sakamoto Yuki、Sato Mayuko、Sato Yoshikatsu、Harada Akihito、Suzuki Takamasa、Goto Chieko、Tamura Kentaro、Toyooka Kiminori、Kimura Hiroshi、Ohkawa Yasuyuki、Hara-Nishimura Ikuko、Takagi Shingo、Matsunaga Sachihiro
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 5914-5914
  • DOI: 10.1038/s41467-020-19621-z
  • Peer Reviewed / Open Access
• [Journal Article] Biogenesis of leaf endoplasmic reticulum body is regulated by both jasmonate-dependent and independent pathways (2019)
  • Author(s): Nakazaki Akiko、Yamada Kenji、Kunieda Tadashi、Tamura Kentaro、Hara-Nishimura Ikuko、Shimada Tomoo
  • Journal Title: Plant Signaling & Behavior Volume: 14 Issue: 8 Pages: 1622982-1622982
  • DOI: 10.1080/15592324.2019.1622982
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction (2019)
  • Author(s): Goto Chieko、Hashizume Shoko、Fukao Yoichiro、Hara-Nishimura Ikuko、Tamura Kentaro
  • Journal Title: Nucleus Volume: 10 Issue: 1 Pages: 81-92
  • DOI: 10.1080/19491034.2019.1603093
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Periplasmic Root-Cap Mucilage in Developing Columella Cells of Arabidopsis thaliana (2019)
  • Author(s): Maeda Kazuki、Kunieda Tadashi、Tamura Kentaro、Hatano Kyoko、Hara-Nishimura Ikuko、Shimada Tomoo
  • Journal Title: Plant and Cell Physiology Volume: - Issue: 6 Pages: 1296-1303
  • DOI: 10.1093/pcp/pcz047
  • Peer Reviewed
• [Journal Article] Leaf Endoplasmic Reticulum Bodies Identified in Arabidopsis Rosette Leaves Are Involved in Defense against Herbivory (2019)
  • Author(s): Nakazaki Akiko、Yamada Kenji、Kunieda Tadashi、Sugiyama Ryosuke、Hirai Masami Yokota、Tamura Kentaro、Hara-Nishimura Ikuko、Shimada Tomoo
  • Journal Title: Plant Physiology Volume: 179 Issue: 4 Pages: 1515-1524
  • DOI: 10.1104/pp.18.00984
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Subcellular localisation of an endoplasmic reticulum-plasma membrane tethering factor, SYNAPTOTAGMIN 1, is affected by fluorescent protein fusion (2019)
  • Author(s): K. Ishikawa, Kentaro Tamura, and T. Shimada
  • Journal Title: Plant Signaling & Behavior Volume: 16 Issue: 12 Pages: 1-4
  • DOI: 10.1080/15592324.2018.1547577
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] ANGUSTIFOLIA regulate the alignment of actin filaments for homeostatic nuclear positioning in Arabidopsis leaves (2019)
  • Author(s): K. Iwabuchi, H. Ohnishi, Kentaro Tamura, Y. Fukao, H. Tsukaya, and I. Hara-Nishimura
  • Journal Title: Plant Physiology Volume: 179 Issue: 1 Pages: 233-247
  • DOI: 10.1104/pp.18.01150
  • Peer Reviewed / Open Access
• [Journal Article] Computational methods for studying the plant nucleus (2018)
  • Author(s): A. Poulet, X. Zhou, Kentaro Tamura, I. Meier, C. Tatout, K. Graumann, and D. Evans
  • Journal Title: Methods in Molecular Biology Volume: 1840 Pages: 205-219
  • DOI: 10.1007/978-1-4939-8691-0_15
  • ISBN: 9781493986903, 9781493986910
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The AP-1 complex is required for proper mucilage formation in Arabidopsis seeds (2018)
  • Author(s): T. Shimada, T. Kunieda, S. Sumi, Y. Koumoto, Kentaro Tamura, K. Hatano, H. Ueda, and I. Hara-Nishimura
  • Journal Title: Plant & Cell Physiology Volume: 59 Pages: 2331-2338
  • DOI: 10.1093/pcp/pcy158
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum (ER) membrane proteins (LUNAPARKs) are required for proper configuration of the cortical ER network in plant cells (2018)
  • Author(s): H. Ueda, N. Ohta, Y. Kimori, T. Uchida, T. Shimada, Kentaro Tamura, and I. Hara-Nishimura
  • Journal Title: Plant & Cell Physiology Volume: 59 Issue: 10 Pages: 1931-1941
  • DOI: 10.1093/pcp/pcy137
  • Peer Reviewed / Open Access
• [Journal Article] Synaptotagmin-associated endoplasmic reticulum-plasma membrane contact sites are localized to immobile ER tubules (2018)
  • Author(s): K. Ishikawa, Kentaro Tamura, H. Ueda, Y. Ito, A. Nakano, I. Hara-Nishimura, and T. Shimada
  • Journal Title: Plant Physiology Volume: 178 Issue: 2 Pages: 641-653
  • DOI: 10.1104/pp.18.00498
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 膜を有さない核内構造体カハールボディはシロイヌナズナの高温への応答に関与している (2022)
  • Author(s): 太田翔平，櫻井貴之，嶋田知生，田村謙太郎
  • Organizer: 第63回日本植物生理学会
• [Presentation] インポーチンα(IMPA)は植物の免疫応答を抑制する (2022)
  • Author(s): 森愛理，Valerie Gaudin，田村謙太郎
  • Organizer: 第63回日本植物生理学会
• [Presentation] 気孔形成をかく乱する小分子化合物 Bubblin の標的因子の解析 (2022)
  • Author(s): 村上知暉，安西慎太郎，阪井裕美子，田村謙太郎，松田佳祐，今井敏郎，大越研人，佐藤安弘， 永野惇，岡義人，松下智直，嶋田知生
  • Organizer: 第63回日本植物生理学会
• [Presentation] The physiological roles of nuclear envelope proteins in plant reproductive organs (2021)
  • Author(s): Kentaro Tamura
  • Organizer: Society for Experimental Biology Annual Main Meeting
  • Int'l Joint Research / Invited
• [Presentation] Functional and spatial coordination between cell and nuclear shape in plants (2021)
  • Author(s): Kentaro Tamura
  • Organizer: Society for Experimental Biology Annual Main Meeting
  • Int'l Joint Research / Invited
• [Presentation] 核膜孔が制御する植物の環境応答 (2021)
  • Author(s): 田村謙太郎
  • Organizer: 第73回日本細胞生物学会大会
  • Invited
• [Presentation] Real time nucleus dynamics in the Arabidopsis single root hair (2021)
  • Author(s): Gaurav Singh, David Pereira, Tamsin A. Spelman, Stephanie Baudrey, Kentaro Tamura, Michael Ryckelynck, Henrik Jonsson, Atef Asnacios and Marie-Edith Chaboute
  • Organizer: 20th HFSP Awardees Meeting
  • Int'l Joint Research
• [Presentation] Characterization of a Cajal body deficient mutant in Arabidopsis thaliana (2021)
  • Author(s): Shohei Ohta, Takayuki Sakurai, Tomoo Shimada, and Kentaro Tamura
  • Organizer: Society for Experimental Biology
  • Int'l Joint Research
• [Presentation] Interaction of the nuclear pore complex and the nucleoskeleton at the plant nuclear periphery (2021)
  • Author(s): Sarah Mermet, Tristan Dubos, Sylvie Tutois, Kentaro Tamura, Christophe Tatout, Emmanuel Vanrobays
  • Organizer: Society for Experimental Biology
  • Int'l Joint Research
• [Presentation] シロイヌナズナにおける核輸送因子インポーチンαファミリーの解析 (2021)
  • Author(s): 森 愛理，Valerie Gaudin, 田村 謙太郎
  • Organizer: 日本植物学会第85回大会
• [Presentation] 温度ストレス応答における核内小器官カハールボディの役割 (2021)
  • Author(s): 太田 翔平, 櫻井 貴之，嶋田 知生，田村 謙太郎
  • Organizer: 日本植物学会第85回大会
• [Presentation] 出芽酵母のエネルギー代謝の改善 (2021)
  • Author(s): 大長 薫，弘埜 陽子，菊川 寛史，田村 謙太郎，原 清敬
  • Organizer: 第73回日本生物工学大会
• [Presentation] GFP蛍光を指標に単離したシロイヌナズナ排水組織マイクロサンプルのRNA-seq解析 (2021)
  • Author(s): 八木宏樹，永野惇，キム・ジュウ，田村謙太郎，望月伸悦，長谷あきら，松下智直，嶋田知生
  • Organizer: 第62回日本植物生理学会年会
• [Presentation] MpBHLH35はゼニゴケ胞子体の蒴柄形成を制御する (2021)
  • Author(s): 守屋健太，白川一，松田頼子，田村謙太郎，西浜竜一，岡義人，松下智直，西村いくこ，河内孝之，嶋田知生
  • Organizer: 第62回日本植物生理学会年会
• [Presentation] 液胞輸送変異体kam2における発芽後の成長阻害の解析 (2021)
  • Author(s): 細川智佳，田村謙太郎，岡義人，松下智直，嶋田知生
  • Organizer: 第62回日本植物生理学会年会
• [Presentation] unctional analysis of putative peptide-coding genes in Marchantia polymorpha (2021)
  • Author(s): Haruaki Kobayashi, Shigeo S. Sugano, Kentaro Tamura, Yoshito Oka, Tomonao Matsushita, Tomoo Shimada
  • Organizer: 第62回日本植物生理学会年会
• [Presentation] 小胞体の構造と機能に関するSUNドメインタンパク質 (2020)
  • Author(s): 田村 謙太郎
  • Organizer: 第93回日本生化学会大会シンポジウム
  • Invited
• [Presentation] Emerging roles for nuclear membrane in signalling pathway in higher plants (2020)
  • Author(s): Kentaro Tamura
  • Organizer: 第43回日本分子生物学会年会ワークショップ
  • Invited
• [Presentation] The nuclear envelope protein KAKU4 affects the migration order of the vegetative nucleus and sperm cells in pollen tubes (2020)
  • Author(s): Chieko Goto, Kentaro Tamura, Hara-Nishimura
  • Organizer: Plant Biology 2020 Worldwide Summit
  • Int'l Joint Research
• [Presentation] シロイヌナズナのカハールボディの機能解析 (2020)
  • Author(s): 太田 翔平, 櫻井 貴之，深尾 陽一朗，嶋田 知生，田村 謙太郎
  • Organizer: 日本植物学会第84回大会
• [Presentation] A novel candidate for plant lamina component in Arabidopsis (2019)
  • Author(s): Kentaro Tamura
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics
  • Int'l Joint Research / Invited
• [Presentation] Nucleocytoplasmic transport functions in environmental responses of higher plants (2018)
  • Author(s): Kentaro Tamura
  • Organizer: Annual Meeting of Molecular Biology Society of Japan
  • Invited
• [Presentation] Dynamic regulation of plant nuclear architecture (2018)
  • Author(s): Kentaro Tamura
  • Organizer: Annual Meeting of the Botanical Society of Japan
  • Invited
• [Presentation] Diverse functions of plant nuclear envelope proteins (2018)
  • Author(s): Kentaro Tamura and Ikuko Hara-Nishimura
  • Organizer: Society for Experimental Biology Annual Main Meeting
  • Int'l Joint Research / Invited