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Interaction between nuclear pore complex and chromatin in plants

Research Project

Project/Area Number 18K06283
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionUniversity of Shizuoka

Principal Investigator

Tamura Kentaro  静岡県立大学, 食品栄養科学部, 准教授 (40378609)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsシロイヌナズナ / 核膜 / 核膜孔 / 遺伝子発現 / ヌクレオポリン / 核ラミナ / クロマチン / 遺伝子発現制御 / 花粉 / サリチル酸 / 病害応答 / クロマチン構造 / 植物免疫
Outline of Final Research Achievements

The nuclear pore complex is a multi-functional channel regulating nucleo-cytoplasmic transport in eukaryotes. The nuclear pore complex is composed of more than 30 nucleoporin proteins in higher plants. In this study, we have revealed interaction between nucleoporin and specific chromatin regions in Arabidopsis and that this interaction depends on salicylic acid signaling. We also established in-vitro assay system of nuclear envelop reconstitution with extracts from plant cultured cells. This system might provide important insights into the molecular machinery of the nuclear envelop interaction with chromatin in plants.

Academic Significance and Societal Importance of the Research Achievements

核膜孔複合体および核膜は真核生物に広く保存された構造であるが,その機能は多岐にわたることが推定されていた.私達がこれまでに見いだしていた植物独自の核膜孔や核膜成分の解析をベースとして研究を展開した.本研究成果では,植物が進化の過程で独自に手に入れた遺伝子発現制御機構および,器官発生における核膜成分の新しい役割と機能を明らかにすることができた.これらの成果は効率的な食料増産を進めるうえで重要な分子基盤となることが期待される.

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (46 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (19 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 19 results,  Open Access: 15 results) Presentation (24 results) (of which Int'l Joint Research: 8 results,  Invited: 9 results)

  • [Int'l Joint Research] INRA/IBMP(フランス)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] INRA/IJPB(フランス)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] University of Clermont Auvergne/IBMP(フランス)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Validation of nuclear pore complex protein-protein interactions by transient expression in plants2022

    • Author(s)
      Fumika Ikeda、Kentaro Tamura
    • Journal Title

      Methods in Molecular Biology

      Volume: 2502 Pages: 235-243

    • DOI

      10.1007/978-1-0716-2337-4_15

    • ISBN
      9781071623367, 9781071623374
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatiotemporal relationship between auxin dynamics and hydathode development in Arabidopsis leaf teeth2021

    • Author(s)
      Yagi Hiroki、Tamura Kentaro、Matsushita Tomonao、Shimada Tomoo
    • Journal Title

      Plant Signaling and Behavior

      Volume: 16 Issue: 12 Pages: 1989216-1989216

    • DOI

      10.1080/15592324.2021.1989216

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation and Physiological Significance of the Nuclear Shape in Plants2021

    • Author(s)
      Goto Chieko、Hara-Nishimura Ikuko、Tamura Kentaro
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 67390-67390

    • DOI

      10.3389/fpls.2021.673905

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro assembly of nuclear envelope in tobacco cultured cells2021

    • Author(s)
      Tamura Kentaro、Ueda Haruko、Hara-Nishimura Ikuko
    • Journal Title

      Nucleus

      Volume: 12 Issue: 1 Pages: 82-89

    • DOI

      10.1080/19491034.2021.1930681

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fluorescent protein-based imaging and tissue-specific RNA-seq analysis of Arabidopsis hydathodes2020

    • Author(s)
      Yagi Hiroki、Nagano Atsushi J、Kim Jaewook、Tamura Kentaro、Mochizuki Nobuyoshi、Nagatani Akira、Matsushita Tomonao、Shimada Tomoo
    • Journal Title

      Journal of Experimental Botany

      Volume: 72 Issue: 4 Pages: 1260-1270

    • DOI

      10.1093/jxb/eraa519

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural and functional relationships between plasmodesmata and plant endoplasmic reticulum?plasma membrane contact sites consisting of three synaptotagmins2020

    • Author(s)
      Ishikawa Kazuya、Tamura Kentaro、Fukao Yoichiro、Shimada Tomoo
    • Journal Title

      New Phytologist

      Volume: 226 Issue: 3 Pages: 798-808

    • DOI

      10.1111/nph.16391

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nuclear pore complex-mediated gene expression in Arabidopsis thaliana2020

    • Author(s)
      Tamura Kentaro
    • Journal Title

      Journal of Plant Research

      Volume: 133 Issue: 4 Pages: 449-455

    • DOI

      10.1007/s10265-020-01177-0

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] The nuclear envelope protein KAKU4 determines the migration order of the vegetative nucleus and sperm cells in pollen tubes2020

    • Author(s)
      Goto Chieko、Tamura Kentaro、Nishimaki Satsuki、Maruyama Daisuke、Hara-Nishimura Ikuko
    • Journal Title

      Journal of Experimental Botany

      Volume: 71 Issue: 20 Pages: 6273-6281

    • DOI

      10.1093/jxb/eraa367

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Subnuclear gene positioning through lamina association affects copper tolerance2020

    • Author(s)
      Sakamoto Yuki、Sato Mayuko、Sato Yoshikatsu、Harada Akihito、Suzuki Takamasa、Goto Chieko、Tamura Kentaro、Toyooka Kiminori、Kimura Hiroshi、Ohkawa Yasuyuki、Hara-Nishimura Ikuko、Takagi Shingo、Matsunaga Sachihiro
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 5914-5914

    • DOI

      10.1038/s41467-020-19621-z

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biogenesis of leaf endoplasmic reticulum body is regulated by both jasmonate-dependent and independent pathways2019

    • Author(s)
      Nakazaki Akiko、Yamada Kenji、Kunieda Tadashi、Tamura Kentaro、Hara-Nishimura Ikuko、Shimada Tomoo
    • Journal Title

      Plant Signaling & Behavior

      Volume: 14 Issue: 8 Pages: 1622982-1622982

    • DOI

      10.1080/15592324.2019.1622982

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction2019

    • Author(s)
      Goto Chieko、Hashizume Shoko、Fukao Yoichiro、Hara-Nishimura Ikuko、Tamura Kentaro
    • Journal Title

      Nucleus

      Volume: 10 Issue: 1 Pages: 81-92

    • DOI

      10.1080/19491034.2019.1603093

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Periplasmic Root-Cap Mucilage in Developing Columella Cells of Arabidopsis thaliana2019

    • Author(s)
      Maeda Kazuki、Kunieda Tadashi、Tamura Kentaro、Hatano Kyoko、Hara-Nishimura Ikuko、Shimada Tomoo
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 6 Pages: 1296-1303

    • DOI

      10.1093/pcp/pcz047

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Leaf Endoplasmic Reticulum Bodies Identified in Arabidopsis Rosette Leaves Are Involved in Defense against Herbivory2019

    • Author(s)
      Nakazaki Akiko、Yamada Kenji、Kunieda Tadashi、Sugiyama Ryosuke、Hirai Masami Yokota、Tamura Kentaro、Hara-Nishimura Ikuko、Shimada Tomoo
    • Journal Title

      Plant Physiology

      Volume: 179 Issue: 4 Pages: 1515-1524

    • DOI

      10.1104/pp.18.00984

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Subcellular localisation of an endoplasmic reticulum-plasma membrane tethering factor, SYNAPTOTAGMIN 1, is affected by fluorescent protein fusion2019

    • Author(s)
      K. Ishikawa, Kentaro Tamura, and T. Shimada
    • Journal Title

      Plant Signaling & Behavior

      Volume: 16 Issue: 12 Pages: 1-4

    • DOI

      10.1080/15592324.2018.1547577

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ANGUSTIFOLIA regulate the alignment of actin filaments for homeostatic nuclear positioning in Arabidopsis leaves2019

    • Author(s)
      K. Iwabuchi, H. Ohnishi, Kentaro Tamura, Y. Fukao, H. Tsukaya, and I. Hara-Nishimura
    • Journal Title

      Plant Physiology

      Volume: 179 Issue: 1 Pages: 233-247

    • DOI

      10.1104/pp.18.01150

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Computational methods for studying the plant nucleus2018

    • Author(s)
      A. Poulet, X. Zhou, Kentaro Tamura, I. Meier, C. Tatout, K. Graumann, and D. Evans
    • Journal Title

      Methods in Molecular Biology

      Volume: 1840 Pages: 205-219

    • DOI

      10.1007/978-1-4939-8691-0_15

    • ISBN
      9781493986903, 9781493986910
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The AP-1 complex is required for proper mucilage formation in Arabidopsis seeds2018

    • Author(s)
      T. Shimada, T. Kunieda, S. Sumi, Y. Koumoto, Kentaro Tamura, K. Hatano, H. Ueda, and I. Hara-Nishimura
    • Journal Title

      Plant & Cell Physiology

      Volume: 59 Pages: 2331-2338

    • DOI

      10.1093/pcp/pcy158

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Endoplasmic reticulum (ER) membrane proteins (LUNAPARKs) are required for proper configuration of the cortical ER network in plant cells2018

    • Author(s)
      H. Ueda, N. Ohta, Y. Kimori, T. Uchida, T. Shimada, Kentaro Tamura, and I. Hara-Nishimura
    • Journal Title

      Plant & Cell Physiology

      Volume: 59 Issue: 10 Pages: 1931-1941

    • DOI

      10.1093/pcp/pcy137

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synaptotagmin-associated endoplasmic reticulum-plasma membrane contact sites are localized to immobile ER tubules2018

    • Author(s)
      K. Ishikawa, Kentaro Tamura, H. Ueda, Y. Ito, A. Nakano, I. Hara-Nishimura, and T. Shimada
    • Journal Title

      Plant Physiology

      Volume: 178 Issue: 2 Pages: 641-653

    • DOI

      10.1104/pp.18.00498

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 膜を有さない核内構造体カハールボディはシロイヌナズナの高温への応答に関与している2022

    • Author(s)
      太田翔平,櫻井貴之,嶋田知生,田村謙太郎
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] インポーチンα(IMPA)は植物の免疫応答を抑制する2022

    • Author(s)
      森愛理,Valerie Gaudin,田村謙太郎
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 気孔形成をかく乱する小分子化合物 Bubblin の標的因子の解析2022

    • Author(s)
      村上知暉,安西慎太郎,阪井裕美子,田村謙太郎,松田佳祐,今井敏郎,大越研人,佐藤安弘, 永野惇,岡義人,松下智直,嶋田知生
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] The physiological roles of nuclear envelope proteins in plant reproductive organs2021

    • Author(s)
      Kentaro Tamura
    • Organizer
      Society for Experimental Biology Annual Main Meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional and spatial coordination between cell and nuclear shape in plants2021

    • Author(s)
      Kentaro Tamura
    • Organizer
      Society for Experimental Biology Annual Main Meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 核膜孔が制御する植物の環境応答2021

    • Author(s)
      田村謙太郎
    • Organizer
      第73回日本細胞生物学会大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Real time nucleus dynamics in the Arabidopsis single root hair2021

    • Author(s)
      Gaurav Singh, David Pereira, Tamsin A. Spelman, Stephanie Baudrey, Kentaro Tamura, Michael Ryckelynck, Henrik Jonsson, Atef Asnacios and Marie-Edith Chaboute
    • Organizer
      20th HFSP Awardees Meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of a Cajal body deficient mutant in Arabidopsis thaliana2021

    • Author(s)
      Shohei Ohta, Takayuki Sakurai, Tomoo Shimada, and Kentaro Tamura
    • Organizer
      Society for Experimental Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interaction of the nuclear pore complex and the nucleoskeleton at the plant nuclear periphery2021

    • Author(s)
      Sarah Mermet, Tristan Dubos, Sylvie Tutois, Kentaro Tamura, Christophe Tatout, Emmanuel Vanrobays
    • Organizer
      Society for Experimental Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナにおける核輸送因子インポーチンαファミリーの解析2021

    • Author(s)
      森 愛理,Valerie Gaudin, 田村 謙太郎
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 温度ストレス応答における核内小器官カハールボディの役割2021

    • Author(s)
      太田 翔平, 櫻井 貴之,嶋田 知生,田村 謙太郎
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 出芽酵母のエネルギー代謝の改善2021

    • Author(s)
      大長 薫,弘埜 陽子,菊川 寛史,田村 謙太郎,原 清敬
    • Organizer
      第73回日本生物工学大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] GFP蛍光を指標に単離したシロイヌナズナ排水組織マイクロサンプルのRNA-seq解析2021

    • Author(s)
      八木宏樹,永野惇,キム・ジュウ,田村謙太郎,望月伸悦,長谷あきら,松下智直,嶋田知生
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] MpBHLH35はゼニゴケ胞子体の蒴柄形成を制御する2021

    • Author(s)
      守屋健太,白川一,松田頼子,田村謙太郎,西浜竜一,岡義人,松下智直,西村いくこ,河内孝之,嶋田知生
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 液胞輸送変異体kam2における発芽後の成長阻害の解析2021

    • Author(s)
      細川智佳,田村謙太郎,岡義人,松下智直,嶋田知生
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] unctional analysis of putative peptide-coding genes in Marchantia polymorpha2021

    • Author(s)
      Haruaki Kobayashi, Shigeo S. Sugano, Kentaro Tamura, Yoshito Oka, Tomonao Matsushita, Tomoo Shimada
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 小胞体の構造と機能に関するSUNドメインタンパク質2020

    • Author(s)
      田村 謙太郎
    • Organizer
      第93回日本生化学会大会シンポジウム
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Emerging roles for nuclear membrane in signalling pathway in higher plants2020

    • Author(s)
      Kentaro Tamura
    • Organizer
      第43回日本分子生物学会年会ワークショップ
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] The nuclear envelope protein KAKU4 affects the migration order of the vegetative nucleus and sperm cells in pollen tubes2020

    • Author(s)
      Chieko Goto, Kentaro Tamura, Hara-Nishimura
    • Organizer
      Plant Biology 2020 Worldwide Summit
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナのカハールボディの機能解析2020

    • Author(s)
      太田 翔平, 櫻井 貴之,深尾 陽一朗,嶋田 知生,田村 謙太郎
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] A novel candidate for plant lamina component in Arabidopsis2019

    • Author(s)
      Kentaro Tamura
    • Organizer
      The 3rd International Symposium on Plant Nuclear Dynamics
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Nucleocytoplasmic transport functions in environmental responses of higher plants2018

    • Author(s)
      Kentaro Tamura
    • Organizer
      Annual Meeting of Molecular Biology Society of Japan
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Dynamic regulation of plant nuclear architecture2018

    • Author(s)
      Kentaro Tamura
    • Organizer
      Annual Meeting of the Botanical Society of Japan
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Diverse functions of plant nuclear envelope proteins2018

    • Author(s)
      Kentaro Tamura and Ikuko Hara-Nishimura
    • Organizer
      Society for Experimental Biology Annual Main Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2018-04-23   Modified: 2023-01-30  

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