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Genetic investigation of Babesia parasites in Japan

Research Project

Project/Area Number 18K06398
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Niikura Aya  国立感染症研究所, 安全管理研究センター, 主任研究官 (10392325)

Project Period (FY) 2018-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsBabesia / Babesia microti group / 次世代シークエンス / 全ゲノムシークエンス / 遺伝的多様性 / 全ゲノム解析 / ピロプラズマ
Outline of Final Research Achievements

We analyzed the genomes of three strains of Babesia microti (Hobetsu, US, and Kobe), which were isolated from field mice and transmitted by different vectors ticks in Japan. The sister species, B. rodhaini, was included in this study. The number of chromosomes was the same as that of the previously reported smallest apicomplexan, B. microti RI strain (6.5 Mb). However, their sizes differed greatly, with a 13.5% gap (6.0 Mb minimum). Gene prediction revealed the presence of tiny introns (19-25 nt), which were scattered throughout the genome. There were differences in size distribution among the strains. This study reveals for the first time the genomic diversity of the B. microti group.

Academic Significance and Societal Importance of the Research Achievements

Babesia microtiは約6億年前に ピロプラズマ目(赤血球に感染し、ダニが媒介する寄生虫)の他の原虫よりも早く共通祖先から分化した。日本で分離された複数のB. microtiゲノム解析から、そのサイズが約6.0Mbとアピコンプレックス門最小であること、僅かな真核生物種でしか見つかっていない~25ntの極小イントロンがゲノム全体に散在していること、特有のミトコンドリア構造をもつこと、これらは系統間で多様であることなどが明らかとなった。さらに解析を進めることで、原虫が効率の良い代謝・増殖能力を獲得し、環境変化に柔軟に適応してきたプロセスを、ゲノム進化から解明できる可能性が見えてきた。

Report

(7 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (15 results)

All 2024 2023 2021 2020 2018

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Open Access: 8 results,  Peer Reviewed: 3 results) Presentation (6 results) (of which Int'l Joint Research: 3 results) Book (1 results)

  • [Journal Article] Genetic investigation of GPI anchored Bd37 orthologs in Babesia divergens group and use of recombinant protein for ecological survey in deer2024

    • Author(s)
      Zamoto-Niikura Aya, Hagiwara Katsuro, Imaoka Koichi, Morikawa Shigeru
    • Journal Title

      BioRxiv

      Volume: -

    • DOI

      10.1101/2024.03.19.585777

    • Related Report
      2023 Annual Research Report
    • Open Access
  • [Journal Article] Genome sequences of four<i>Ixodes</i>species expands understanding of tick evolution2024

    • Author(s)
      Alexandra Cerqueira de Araujo, Anthony Bretaudeau, Karine Labadie, Nachida Tadrent, Benjamin Istace, Salima Kritli, Corinne Cruaud, Robert Olaso, Aya Zamoto-Niikura(省略)Claude Rispe
    • Journal Title

      BioRxiv

      Volume: 0

    • DOI

      10.1101/2024.02.29.581698

    • Related Report
      2023 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] The presence of Ixodes pavlovskyi and I. pavlovskyi borne microorganisms in Rishiri Island: an ecological survey2023

    • Author(s)
      Zamoto-Niikura Aya, Saigo Akiko, Sato Masahiko, Kobayashi Hirotaka, Sasaki Mizuki, Nakao Minoru, Suzuki Tadaki, Morikawa Shigeru
    • Journal Title

      mSphere

      Volume: 8 Issue: 6

    • DOI

      10.1128/msphere.00213-23

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Finding of Ixodes pavlovskyi (Acari, Ixodidae) from Oriental Greenfinch, Chloris sinica, on Rishiri Island, northren Hokkaido2021

    • Author(s)
      Masahiko Sato, Mamoru Takahashi and Aya Zamoto-Niikura.
    • Journal Title

      Rishri Studies

      Volume: 40 Pages: 25-28

    • Related Report
      2020 Research-status Report
    • Open Access
  • [Journal Article] Epidemiological Survey of <i>Babesia divergens</i> Asia Lineage in Wild Sika Deer (<i>Cervus nippon</i>) by Using Direct PCR in Japan2020

    • Author(s)
      Zamoto-Niikura Aya、Hagiwara Katsuro、Imaoka Koichi、Morikawa Shigeru、Ishihara Chiaki、Hanaki Ken-Ichi
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 73 Issue: 1 Pages: 68-71

    • DOI

      10.7883/yoken.JJID.2019.096

    • NAID

      130007789148

    • ISSN
      1344-6304, 1884-2836
    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Investigation of Tick-borne Pathogens in Ticks on Rishiri Island, Hokkaido, Japan2020

    • Author(s)
      Zamoto-Niikura A., M. Sato, H. Kawabata, K. Sato-Okubo, S. Ando, C. Ishihara & K. Hanaki.
    • Journal Title

      Rishri Studies

      Volume: 39 Pages: 41-46

    • Related Report
      2020 Research-status Report
    • Open Access
  • [Journal Article] Investigation of Tick-borne Pathogens in Ticks on Rishiri Island, Hokkaido, Japan2020

    • Author(s)
      Zamoto-Niikura A., M. Sato, H. Kawabata, K. Sato-Okubo, S. Ando, C. Ishihara & K. Hanaki
    • Journal Title

      Rishiri Studies

      Volume: 39 Pages: 41-46

    • Related Report
      2019 Research-status Report
    • Open Access
  • [Journal Article] Analysis of antigen-antibody cross-reactivity among lineages and sublineages of Babesia microti parasites using human babesiosis specimens2018

    • Author(s)
      Sayama Yusuke、Zamoto-Niikura Aya、Matsumoto Chieko、Saijo Masayuki、Ishihara Chiaki、Matsubayashi Keiji、Nagai Tadashi、Satake Masahiro
    • Journal Title

      Transfusion

      Volume: 58 Issue: 5 Pages: 1234-1244

    • DOI

      10.1111/trf.14558

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 新倉(座本)綾、高橋弘喜、豊田敦、鈴木里和、筏井宏実、花木賢一2024

    • Author(s)
      アピコンプレクサ門最小のBabesia rodhainiのゲノム解析
    • Organizer
      第167回日本獣医学会学術集会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Babesia原虫の抗体保有調査2023

    • Author(s)
      新倉(座本)綾 , 本間健一 , 中山健史 , 小林進太郎 , 好井健太郎 , 好井健太郎
    • Organizer
      第166回日本獣医学会学術集会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Development ELISA system based on recombinant Bd37 proteins of Babesia divergens Asia-lineage2021

    • Author(s)
      Aya Zamoto-Niikura
    • Organizer
      Human Babesiosis Meeting III
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] シリアンハムスターにおけるヒトバベシア症原因原虫Babesia microti (Kobe-typeおよびUS-type)の感染動態2018

    • Author(s)
      佐山勇輔、新倉(座本)綾、松本千惠子、西條政幸、松林圭二、永井正、佐竹正博
    • Organizer
      第66回日本輸血・細胞治療学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Genetic polymorphism and amino acid sequence variation of Babesia divergens Bd37 in Ixodes persulcatus and Cervus nippon in Japan2018

    • Author(s)
      A. Zamoto-Niikura, Imaoka K, Morikawa S, Hagiwara K, Ishihara C, Hanaki K
    • Organizer
      Human babesiosis meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular analysis of Bd37 of Babesia divergens in Japan.2018

    • Author(s)
      Zamoto-Niikura A, Imaoka K, Morikawa S, Hagiwara K, Ishihara C, Hanaki K.
    • Organizer
      European Congress of Clinical Microbiology and Infectious Diseases
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] <日本では稀な感染症> ヒトバベシア症2024

    • Author(s)
      バムサジャーナル
    • Total Pages
      7
    • Publisher
      バイオメディカルサイエンス研究会
    • Related Report
      2023 Annual Research Report

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Published: 2018-04-23   Modified: 2025-01-30  

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