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Genetic variation of complete cleistogamous species in Gastrodia

Research Project

Project/Area Number 18K06408
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionFukushima University

Principal Investigator

Kaneko Shingo  福島大学, 共生システム理工学類, 准教授 (30635983)

Co-Investigator(Kenkyū-buntansha) 末次 健司  神戸大学, 理学研究科, 准教授 (70748839)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords完全閉花授粉植物 / ヤツシロラン / 遺伝的多様性 / 自殖 / 完全閉花受粉植物 / マイクロサテライトマーカー / 種間雑種 / ヤツシロラン類
Outline of Final Research Achievements

In this study, we performed genetic analysis on two complete cleistogamous species and their ancestral species using newly developed microsatellite markers. Microsatellite analysis suggested the loss their variation at the microsatellite locus and the fixation to one allele in two complete cleistogamous species Gastrodia takeshimensis and G. kuroshimensis. Their ancestral species with opened flowers, G. nipponica and G. fontinalis, showed multiple alleles at the species or population level in several microsatellite loci, but their allelic variation was low. It is suggested that they are mainly propagated by self-fertilization, although they have open flowers. Self-breeding may have been established before the cleistogamous flower evolution in Gastrodia species.

Academic Significance and Societal Importance of the Research Achievements

本研究において対象とする完全閉花受粉植物は、開放花を全くつけないだけでなく、光合成を行わず、完全に菌類に寄生する絶対菌従属栄養植物でもある。完全閉花受粉植物の新種の発見は、学会はもちろん一般市民にも驚きをもって迎えられている。“植物”という言葉から通常想起される概念と矛盾する特色をもった植物に関する本研究は、その種についての理解を深めるだけでなく、生物の驚くべき生態や進化の可能性について示す格好の事例である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (2 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] New microsatellite markers recognize differences in tandem repeats among four related <i>Gastrodia</i> species (Orchidaceae)2019

    • Author(s)
      Kenji Ogaki, Kenji Suetsugu, Keiju Kishikawa, Daisuke Kyogoku, Kohtaroh Shutoh, Yuji Isagi, Shingo Kaneko
    • Journal Title

      Genes & Genetic Systems

      Volume: 94 Issue: 5 Pages: 225-229

    • DOI

      10.1266/ggs.19-00025

    • NAID

      130007760287

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2019-10-01
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of microsatellite markers for the completely cleistogamous species <i>Gastrodia takeshimensis</i> (Orchidaceae) that are transferable to its chasmogamous sister <i>G. nipponica</i>2019

    • Author(s)
      Kishikawa K, Suetsugu K, Kyogoku D, Ogaki K, Iga D, Shutoh K, Isagi Y, Kaneko S
    • Journal Title

      Genes & Genetic Systems

      Volume: 94 Issue: 2 Pages: 95-98

    • DOI

      10.1266/ggs.18-00057

    • NAID

      130007638722

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2019-04-01
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] New natural hybrid in the genus Gastrodia: Gastrodia × nippo-uraiensis (Orchidaceae) from Yakushima Island, Japan.2018

    • Author(s)
      Suetsugu K, Hsu TC, Kaneko S
    • Journal Title

      Taiwania

      Volume: 63 Pages: 220-226

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Genetic variation of newly described complete cleistogamous species I-Microsatellite allele fixation in Gastrodia takeshimensis-.2018

    • Author(s)
      Kishikawa K, Suetsugu K, Ogaki K, Kaneko S
    • Organizer
      The 2nd International Academic Conference on the Formation Mechanism of Plant Diversity and Conservation of Endangered Plants in East Asia
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Genetic variation of newly described complete cleistogamous species II- Microsatellite allele difference between cleistogamous Gastrodia kuroshimensis and its sister species-.2018

    • Author(s)
      Ogaki K, Suetsugu K, Kishikawa K, Kaneko S
    • Organizer
      The 2nd International Academic Conference on the Formation Mechanism of Plant Diversity and Conservation of Endangered Plants in East Asia
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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