Uncovering the protein delivery mechanism in synaptic plasticity
Project/Area Number |
18K06498
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 46010:Neuroscience-general-related
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Research Institution | Kobe Pharmaceutical University (2020-2021) Center for Novel Science Initatives, National Institutes of Natural Sciences (2018-2019) |
Principal Investigator |
Nakayama Kei 神戸薬科大学, 薬学部, 助教 (40553590)
|
Project Period (FY) |
2018-04-01 – 2022-03-31
|
Project Status |
Completed (Fiscal Year 2021)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | シナプス可塑性 / 細胞内小器官 / 微小管 / 局所翻訳 / シナプス可塑的変化 / 可塑的変化 |
Outline of Final Research Achievements |
To test whether organelles involved in secretory pathway change their localizations during synaptic plasticity, live imaging of ER and ERGIC was performed. These organelles accumulated in the vicinity of the stimulated-spine. Microtubules play important roles in the motility of organelles in dendritic shaft. I therefore examined whether the dynamics of microtubules is involved in the synaptic plasticity. Spines are enlarged by the treatment with Nocodazole. In contrast, stimulation-induced change in spine was suppressed by Taxol treatment. These results suggested that the dynamics of microtubules is involved in the synaptic plasticity.
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Academic Significance and Societal Importance of the Research Achievements |
本研究において、シナプス刺激に応じた局所翻訳の活性化と協調して細胞内小器官の局在が制御されることが明らかになった。さらに、それら細胞内小器官の局在制御に関わる微小管動態がシナプスの可塑的変化へ関与することも示唆された。シナプスの可塑的変化は記憶形成の細胞基盤であるため、これらの研究成果は、長期記憶形成機構の理解に貢献しうると期待される。
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Report
(5 results)
Research Products
(6 results)
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[Journal Article] Seasonal changes in NRF2 antioxidant pathway regulates winter depression-like behavior2020
Author(s)
Nakayama T, Okimura K, Shen J, Guh Y-J, Tamai K, Shimada A, Minou S, Okushi Y, Shimmura T, Furukawa Y, Kadofusa N, Sato A, Nishimura T, Tanaka M, Nakayama K, Shiina N, Yamamoto N, Loudon A.S, Nishiwaki-Ohkawa T, Shinomiya A, Nabeshima T, Nakane Y, and Yoshimura T
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Journal Title
Proc. Natl. Acad. Sci. U.S.A.
Volume: -
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] RNG105/caprin1, an RNA granule protein, regulates structural spine plasticity and is required for long-term memory formation2018
Author(s)
Kei Nakayama, Manabu Abe, Maya Yamazaki, Akihiro Fujikawa, Masaharu Noda, Akira Futatsugi, Katsuhiko Mikoshiba, Kenji Sakimura, Nobuyuki Shiina
Organizer
The 48th Society for Neuroscience Annual Meeting
Related Report
Int'l Joint Research