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Are neuronal aggregates induced by Reelin the starting point of neocortical layers?

Research Project

Project/Area Number 18K06842
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 48010:Anatomy-related
Research InstitutionKeio University

Principal Investigator

Hayashi Kanehiro  慶應義塾大学, 医学部(信濃町), 講師 (60373354)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords大脳新皮質 / 層構造 / Reelin / N-カドヘリン / 神経細胞 / 細胞凝集 / リーリン / 脂質ラフト / Dab1 / Dbnl / 発生
Outline of Final Research Achievements

In the developing mammalian neocortex, neurons born in or near the ventricular zone migrate to just beneath the Marginal Zone below the brain surface, and then they subsequently accumulate with each other. In this study, we aimed to elucidate the molecular mechanism of this neuronal accumulation.
We identified cellular adhesion-related molecules that function downstream of the secreted protein Reelin, and found that their translocation into lipid rafts leads to N-cadherin-dependent cellular adhesion. We also examined a Reelin point mutant found in a pachygyria patient in terms of the extracellular secretory ability, receptor binding affinity, and effects on Reelin-induced neuronal aggregate formation, and clarified the cause of the dysfunction.

Academic Significance and Societal Importance of the Research Achievements

哺乳類大脳新皮質の6層構造は脳の高次機能を担っており、その破綻は精神疾患の要因となりうるため、6層構造形成機構の解明は重要な課題であるが、いまだ明らかにされていない。本研究では、移動直後の神経細胞の挙動に着目して、Reelinを起点として神経細胞が互いに集合する分子メカニズムの一端を解明した。またReelinは複数の精神疾患の原因遺伝子として同定されており、その発症メカニズムを明らかにすることは医学的に急務である。本研究でPachygyria患者で見つかったReelin変異の機能不全のメカニズムを明らかにでき、この結果は、病因解明や他精神疾患の原因究明に繋がる可能性がある。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (16 results)

All 2022 2021 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (13 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Development of intensiometric indicators for visualizing N-cadherin interaction across cells Communications Biology2022

    • Author(s)
      Kanadome T, Hayashi K, Seto Y, Eiraku M, Nakajima K, Nagai T, Matsuda T
    • Journal Title

      Communications Biology

      Volume: 5 Issue: 1 Pages: 1065-1065

    • DOI

      10.1038/s42003-022-04023-2

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Both excitatory and inhibitory neurons transiently form clusters at the outermost region of the developing mammalian cerebral neocortex2019

    • Author(s)
      Shin Minkyung、Kitazawa Ayako、Yoshinaga Satoshi、Hayashi Kanehiro、Hirata Yukio、Dehay Colette、Kubo Ken‐ichiro、Nakajima Kazunori
    • Journal Title

      Journal of Comparative Neurology

      Volume: 527 Issue: 10 Pages: 1577-1597

    • DOI

      10.1002/cne.24634

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Drebrin-like (Dbnl) Controls Neuronal Migration via Regulating N-Cadherin Expression in the Developing Cerebral Cortex2018

    • Author(s)
      Inoue Seika、Hayashi Kanehiro、Fujita Kyota、Tagawa Kazuhiko、Okazawa Hitoshi、Kubo Ken-ichiro、Nakajima Kazunori
    • Journal Title

      The Journal of Neuroscience

      Volume: 39 Issue: 4 Pages: 678-691

    • DOI

      10.1523/jneurosci.1634-18.2018

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 分泌性糖タンパク質リーリンによるN-カドヘリン輸送制御2022

    • Author(s)
      林周宏、井上聖香、久保健一郎、中尾信彦、安達泰治、仲嶋一範
    • Organizer
      第45回日本神経科学大会・第64回日本神経化学会大会・第32回日本神経回路学会大会合同大会 (Neuro2022)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 哺乳類発生期大脳新皮質で移動神経細胞が辺縁帯直下で移動を停止するメカニズム2022

    • Author(s)
      林光太郎、林周宏、本田岳夫、Nadia Bahi-Buisson、Alessandra Pierani、仲嶋一範
    • Organizer
      第45回日本神経科学大会・第64回日本神経化学会大会・第32回日本神経回路学会大会合同大会 (Neuro2022)
    • Related Report
      2022 Annual Research Report
  • [Presentation] How do neurons stop migration just beneath the marginal zone in the developing neocortex?2022

    • Author(s)
      Kotaro Hayashi, Kanehiro Hayashi, Takao Honda, Nadia Bahi-Buisson, Alessandra Pierani, and Kazunori Nakajima
    • Organizer
      International Symposium: Development and Plasticity of the Brain
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 分泌性糖タンパク質ReelinによるN-cadherin動態制御2021

    • Author(s)
      林周宏、久保健一郎、仲嶋一範
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 移植と新規開発した細胞減少培養系を用いた海馬CA1および大脳新皮質錐体細胞の移動様式を決定する要因の探索2021

    • Author(s)
      北澤彩子、吉永怜史、シン ミンギョン、林周宏、佐野ひとみ、大石康二、久保健一郎、仲嶋一範
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 分泌性糖タンパク質ReelinによるN-cadherinの制御機構2019

    • Author(s)
      林 周宏 、井上聖香、仲尾信彦、久保健一郎、安達泰治、仲嶋一範
    • Organizer
      第42回日本神経科学大会・第62回日本神経化学会大会合同大会(Neuro2019)
    • Related Report
      2019 Research-status Report
  • [Presentation] マウス海馬CA1と大脳新皮質錐体細胞の放射状グリア線維依存的な移動様式の違いについての解析2019

    • Author(s)
      北澤彩子、シン ミンギョン、林周宏、久保健一郎、仲嶋一範
    • Organizer
      第42回日本神経科学大会・第62回日本神経化学会大会合同大会(Neuro2019)
    • Related Report
      2019 Research-status Report
  • [Presentation] 発生中の大脳新皮質において辺縁帯直下に認める比較的未成熟な神経細胞の凝集構造についての知見2019

    • Author(s)
      久保健一郎、シン ミンギョン、北澤彩子、吉永怜史、林周宏、仲嶋一範
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 異所移植による発生期のマウス海馬CA1及び大脳新皮質の移動についての解析2019

    • Author(s)
      北澤彩子、シン ミンギョン、林周宏、久保健一郎、仲嶋一範
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing mouse cerebral neocortex2018

    • Author(s)
      Seika Inoue, Kanehiro Hayashi, Kyota Fujita, Kazuhiko Tagawa, Hitoshi Okazawa, Ken-ichiro Kubo, and Kazunori Nakajima
    • Organizer
      2018 The American Society for Cell Biology (ASCB) Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Reelin controls N-cadherin-dependent neuronal adhesion by a couple of mechanisms in the developing mouse cortex2018

    • Author(s)
      Kanehiro Hayashi, Nobuhiko Nakao, Seika Inoue, Taiji Adachi, Ken-ichiro Kubo, and Kazunori Nakajima
    • Organizer
      第40回日本生物学的精神医学会・第61回日本神経化学会大会 合同年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Reelin-DBNL signaling regulates neuronal migration via N-cadherin/α-N-catenin complex in the intermediate zone and multipolar cell accumulation zone of the developing mouse cerebral neocortex2018

    • Author(s)
      Seika Inoue, Kanehiro Hayashi, Kyota Fujita, Kazuhiko Tagawa, Hitoshi Okazawa, Ken-ichiro Kubo, and Kazunori Nakajima
    • Organizer
      第40回日本生物学的精神医学会・第61回日本神経化学会大会 合同年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 発生期哺乳類大脳皮質における神経細胞移動は癌細胞の間葉上皮転換と同じ仕組みを持つか?2018

    • Author(s)
      林周宏、井上聖香、久保健一郎、仲嶋一範
    • Organizer
      第37回分子病理学研究会はがくれシンポジウム
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2025-01-30  

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