Elucidation of hepatocyte volume regulation and liver regeneration mechanism through TRPM2 activity.
| Project/Area Number |
18K06864
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 48020:Physiology-related
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細胞容積調節能 / 細胞容積調節 / TRP / 肝臓 / 機械刺激 / イオンチャネル |
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| Outline of Final Research Achievements |
Among the mammalian organs, the liver is known to have a particularly high regenerative capacity. There are many unclear points at the cellular level regarding the extraordinary regeneration mechanism of hepatocytes. Therefore, the purpose of this study was to elucidate the molecular mechanism of activation of TRP that works in the process of increasing cell volume, its role in liver tissue, and the mechanism of regeneration from liver damage. This research project explored detailed cell proliferation, cell viability, and cell growth mechanism using cell lines and expression cells, further isolate hepatocytes and activate TRP and its peripheral molecules in individual cells. By exploring the molecular mechanism and measuring in situ cell volume in vivo using a newly developed technology, it was clarified that TRP is involved in the hepatocyte proliferation mechanism.
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| Academic Significance and Societal Importance of the Research Achievements |
肝硬変、肝がんの患者数は日本で推定11万人が罹患しているといわれている（厚生労働省健康局資料, WHO2008）。肝炎ウイルス感染やアルコール多飲、脂肪沈着などが引き起こす肝硬変は、肝臓病が多い国民の健康を害する大きな要因の一つであるが、有効な治療法がない。そのために、本研究結果は肝細胞における細胞容積増大にTRPの活性化分子機構を解明したことで、これらの有効な再生を促す治療法の開発へ重要な基盤情報を与え、その発展に寄与することが期待される。
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] SGLT2 inhibitor ipragliflozin attenuates breast cancer cell proliferation2020
Author(s)
Komatsu Shiho、Nomiyama Takashi、Numata Tomohiro、Kawanami Takako、Hamaguchi Yuriko、Iwaya Chikayo、Horikawa Tsuyoshi、Fujimura-Tanaka Yuki、Hamanoue Nobuya、Motonaga Ryoko、Tanabe Makito、Inoue Ryuji、Yanase Toshihiko、Kawanami Daiji
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Journal Title
Endocr J
Volume: 67
Issue: 1
Pages: 99-106
DOI
NAID
ISSN
0918-8959, 1348-4540
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] TRPM7 channels mediate spontaneous Ca2+ fluctuations in growth plate chondrocytes that promote bone development.2019
Author(s)
Qian N, Ichimura A, Takei D, Sakaguchi R, Kitani A, Nagaoka R, Tomizawa M, Miyazaki Y, Miyachi H, Numata T, Kakizawa S, Nishi M, Mori Y, Takeshima H.
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Journal Title
Sci Signal.
Volume: 12
Pages: 4847-4847
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Cell Volume-Activated and -Correlated Anion Channels in Mammalian Cells: Their Biophysical, Molecular and Pharmacological Properties.2019
Author(s)
Y. Okada, T. Okada, K.S. Numata, M.R. Islam, Y.A. Akatsuka, T. Numata, M. Kubo, T. Shimizu, R.S. Kurbannazarova, Y. Marunaka, and R.Z. Sabirov
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Journal Title
Pharmacol Rev
Volume: 71
Pages: 49-88
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Roles of volume-regulatory anion channels, VSOR and Maxi-Cl, in apoptosis, cisplatin resistance, necrosis, ischemic cell death, stroke and myocardial infarction2019
Author(s)
Yasunobu Okada, Tomohiro Numata, Kaori Sato-Numata, Ravshan Z. Sabirov, Hongtao Liu, Shin-ichiro Mori, and Shigeru Morishima
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Journal Title
Curr Top Membr .
Volume: 印刷中
Pages: 205-283
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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