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Analysis of transcription-coupled mechanism via TREX-2 component GANP

Research Project

Project/Area Number 18K07173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49070:Immunology-related
Research InstitutionOsaka University

Principal Investigator

MAEDA KAZUHIKO  大阪大学, 免疫学フロンティア研究センター, 特任准教授(常勤) (20332869)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsRNA輸送 / 胚中心 / R-loop / AID / R-koop
Outline of Final Research Achievements

During transcription, a DNA-RNA hybrid (R-loop) is transiently formed. The newly transcripts must be rapidly released from the template DNA and transported to the cytoplasm via mRNA transporters. It has been suggested that activation-induced cytidine deaminase (AID) acts on the R-loop during transcription. We investigated whether GANP, a component of the mRNA export complex TREX-2 that interacts with AID, directly affects R-loop formation using antibodies against endogenous GANP. As a result, we were not able to identify it in the antibody gene region. In the future, we plan to investigate GANP mutants that capture transcription in a forced expression system.

Academic Significance and Societal Importance of the Research Achievements

R-ループ形成は全ての遺伝子の転写時に通過するものであり、特に抗体遺伝子座ではB細胞の抗体産生を支える重要な仕組みの一つである。この仕組みの破堤は、容易くDNA損傷を招くとともに、他の細胞種では多数の疾患の要因ともなっている。R-ループから出た正常な転写物がmRNA輸送体へと運ばれていく一方で、mRNAの品質管理で異常となった転写物は、内在性のRNA分解酵素によって分解されて解消されていくと考えられている。本研究の成果は、基礎的解析に留まらず、遺伝的安定性の維持に関わるような疾患や調節機構の解明に繋がり、重要な役割を果たすと考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2021 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Control of RNA Stability in Immunity2021

    • Author(s)
      Akira S and Maeda K.
    • Journal Title

      Annu Rev Immunol

      Volume: 印刷中 Issue: 1 Pages: 481-509

    • DOI

      10.1146/annurev-immunol-101819-075147

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Innate immunity in allergy2019

    • Author(s)
      Maeda K, Caldez MJ, Akira S
    • Journal Title

      Allergy

      Volume: 74 Issue: 9 Pages: 1660-1674

    • DOI

      10.1111/all.13788

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regnase-1 controls colon epithelial regeneration via regulation of mTOR and purine metabolism2018

    • Author(s)
      Nagahama, Y. Shimoda, M. Mao, G. Singh, S. K. Kozakai, Y. Sun, X. Motooka, D. Nakamura, S. Tanaka, H. Satoh, T. Maeda, K. Akira, S.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 115 Issue: 43 Pages: 11036-11041

    • DOI

      10.1073/pnas.1809575115

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Automatic cell type identification by transferring information from public transcriptome data.2019

    • Author(s)
      Diez D, Maeda K, Teraguchi S:
    • Organizer
      Single Cell Biology: EMBO Workshop
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification of immune cells in single cell transcriptomics by transferring information from public datasets.2019

    • Author(s)
      Diez D, Martin J. Loza-Lopez, Maeda K, Teraguchi S:
    • Organizer
      The 18th Awaji International Forum on Infection and Immunity
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 免疫担当細胞におけるシングルセル解析の有効性と実用性2019

    • Author(s)
      前田和彦
    • Organizer
      Single-Cell 2019 OSAKAセミナー
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Endoribonuclease Regnase-1 controls colon epithelial regeneration via regulation of mTOR and purine metabolism2019

    • Author(s)
      Maeda, K. Nagahama, Y. Shimoda, M. Mao, G. Akira, S.
    • Organizer
      The 20th Takeda Science Foundation Symposium on Bioscience RNA Neobiology
    • Related Report
      2018 Research-status Report
  • [Presentation] GANP interacts with translation initiation complex for enhancing c-Myc expression in B cells2018

    • Author(s)
      Singh, S. K. Shimoda, M. Maeda, K. Sakaguchi, N.
    • Organizer
      The 47th Annual Meeting of the Japanese Society for Immunology
    • Related Report
      2018 Research-status Report
  • [Presentation] Metabolic control of Regnase-1 in colon epithelial regeneration2018

    • Author(s)
      Nagahama, Y. Shimoda, M. Kozakai, Y. Tanaka, H. Satoh, T. Maeda, K. Akira, S.
    • Organizer
      The 47th Annual Meeting of the Japanese Society for Immunology
    • Related Report
      2018 Research-status Report
  • [Book] 免疫・炎症疾患のすべて (日本医師会生涯教育シリーズ)2020

    • Author(s)
      監修:竹内勤 編集:渥美達也 岡田浩一 金子裕子 熊ノ郷淳 黒川峰夫 藤尾圭志(担当:分担執筆, 範囲:抗体産生)
    • Total Pages
      368
    • Publisher
      診断と治療社
    • ISBN
      4787824775
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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