Repertoirome analysis of antibody genes of peripheral blood memory B cells for qualitative evaluation of tumor associated immune response
Project/Area Number |
18K07334
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 50020:Tumor diagnostics and therapeutics-related
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Research Institution | Hoshi University |
Principal Investigator |
Tsuiji Makoto 星薬科大学, 薬学部, 准教授 (90302611)
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Project Period (FY) |
2018-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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Keywords | がん免疫 / 免疫状態 / 抗体 / レパトア / 免疫記憶 / バイオマーカー / 末梢血 / scFv-Fc / がん / T細胞抗原受容体 / 可変部 / 記憶B細胞 / 抗体遺伝子 |
Outline of Final Research Achievements |
In cancer-bearing model mice, the abundance ratio of B220-positive and CD4-positive cells in peripheral blood changes correlate with increasing tumor volume. It also clarified the changes in the repertoire of the variable region of antibody genes (BCRs) and the α chain and β chain of T cell receptor genes (TCRs). Furthermore, to verify their binding specificity, we constructed the single-chain antibody (scFv-Fc) and scTCR-Fc. In the future, we plan to continue building a platform to utilize the obtained variable part as a new molecular-targeted drug discovery drug.
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Academic Significance and Societal Importance of the Research Achievements |
抗体医薬品、がんワクチン、免疫療法などの選択基準や治療の継続性の判断基準が不足している現在、個別の免疫状態に対応したプレシジョン医療への移行が求められている。そこで担がんモデルマウスにて、独自に確立した免疫応答の質的評価となるB細胞とT細胞の可変部領域の多様性解析を進めたところ、免疫状態に応じて変化することを明らかにした。さらにそれらの抗原認識特異性を明らかにするために一本鎖抗体(scFv-Fc)およびscTCR-Fcの構築に成功した。今後は、得られた可変部を新たな分子標的医薬品として創薬に活用することを目指しプラットフォームの構築を展開する予定である。
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Report
(5 results)
Research Products
(35 results)
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[Journal Article] Immunomodulatory Activity of an Aqueous Extract of Mycelium of an Endophytic-Like Fungus, Ustilago esculenta2021
Author(s)
Makoto Tsuiji, Junko Nagasaka, Yoshimasa Yajima, Keita Nishiumi, Yasutaka Kojima, Miho Amemiya, Wakana Ogura, Takaaki Moriyama, Tomoko Sakai, Teruaki Oku, Tsutomu Tsuji
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Journal Title
BPB Reports
Volume: 4 (4)
Pages: 98-102
NAID
Related Report
Peer Reviewed
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[Presentation] Proenkephalin+ regulatory T cells expanded by ultraviolet B exposure maintain skin homeostasis with a healing function2021
Author(s)
Hiroaki Shime, Mizuyu Odanaka, Makoto Tsuiji, Masaki Imai, Yoshiaki Yasumizu, Ryuta Uraki, Anthony JB, Hidehiro Fukuyama, Naganari Ohkura, Shimon Sakaguchi, Akimichi Morita, Sayuri Yamazaki
Organizer
第50回 日本免疫学会
Related Report
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[Presentation] Skin regulatory T cells expanded by ultraviolet B exposure have a unique gene expression profile compared to other tissue Treg cells2021
Author(s)
Mizuyu Odanaka, Hiroaki Shime, Makoto Tsuiji, Masaki Imai, Yoshiaki Yasumizu, Ryuta Uraki, Anthony JB, Hidehiro Fukuyama, Naganari Ohkura, Shimon Sakaguchi, Akimichi Morita, Sayuri Yamazaki
Organizer
第50回 日本免疫学会
Related Report
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[Presentation] ヒト由来細胞を利用した新規発熱性物質試験法の開発2020
Author(s)
奥輝明, 安藤祐介, 人見祐基, 築地信, 亀井淳三, 林克彦, 菊池裕, 菊池裕, 工藤由起子, 伊豆津健一, 辻勉
Organizer
日本薬学会年会140th
Related Report
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