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Elucidation of the mechanism of spontaneous prion production in sporadic and familial prion diseases

Research Project

Project/Area Number 18K07393
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 51030:Pathophysiologic neuroscience-related
Research InstitutionUniversity of Miyazaki

Principal Investigator

IMAMURA MORIKAZU  宮崎大学, 医学部, 准教授 (10391442)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords孤発性プリオン病 / 遺伝性プリオン病 / プリオン / 試験管内変換 / PMCA / 試験管内プリオン生成 / 補因子 / 異常プリオン蛋白質 / プリオン病
Outline of Final Research Achievements

In this study, we established an in vitro system for efficient spontaneous conversion of recombinant prion protein and recombinant prion protein carrying the causative gene mutation of familial prion diseases into prion-like structures. Bioassays showed that these products actually functioned as prions, and we concluded that we had successfully constructed an in vitro model of solitary and hereditary prion diseases. We also applied this in vitro prion spontaneous generation system to reveal that one of the reasons for the generation of prion strains with different properties is the difference in the composition of cofactors involved in structural transformation.

Academic Significance and Societal Importance of the Research Achievements

本課題では、孤発性および遺伝性プリオン病の試験管内モデルの構築に成功した。この実験系は、未だ明らかになっていない正常プリオン蛋白質からプリオンへの構造変換メカニズムを解明するために非常に有用である。さらに、この実験系はプリオン病の治療・予防薬のスクリーニング系として用いることができる。その他に本課題では、性状が異なるプリオン株が生成する要因のひとつに構造変換に関わる補因子の組成の違いがあることを明らかにした。この結果は、ある一種類の動物種から異なる性状をもつプリオン株が複数生じるメカニズムを解明するための重要な手がかりになると考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] First case of atypical scrapie in a goat in Japan2019

    • Author(s)
      Matsuura Y, Miyazawa K, Imamura M, Yokoyama T, Iwamaru Y.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 81 Issue: 7 Pages: 986-989

    • DOI

      10.1292/jvms.18-0710

    • NAID

      130007677812

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Isolation of hidden minor prion conformers from classical scrapie isolates in advanced protein misfolding cyclic amplification in the presence of arginine ethyl ester.2019

    • Author(s)
      Imamura M, Miyazawa K, Matsuura Y, Iwamaru Y, Kitamoto T, Mohri S, Takatsuki H, Mori T, Atarashi R
    • Organizer
      APPS2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Simultaneous addition of digitonin, heparin and arginine ethyl ester improves in vitro amplification of PrPSc derived from various prion strains.2018

    • Author(s)
      Imamura M, Tabeta N, Matsuura Y, Iwamaru Y, Kitamoto T, Ma J, Mohri S, Murayama Y, Takatsuki H, Mori T, Atarashi R
    • Organizer
      APPS 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Highly sensitive detection of PrPSc derived from various prion strains by simultaneous addition of digitonin, heparin and arginine ethyl ester to protein misfolding cyclic amplification.2018

    • Author(s)
      Imamura M, Tabeta N, Matsuura Y, Iwamaru Y, Kitamoto T, Ma J, Mohri S, Murayama Y, Takatsuki H, Mori T, Atarashi R
    • Organizer
      Prion 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] In vitro approach to estimate the human transmission risk of prions2018

    • Author(s)
      Iwamaru Y, Imamura M, Matsuura Y, Miyazawa K, Yokoyama T
    • Organizer
      Prion 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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