• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Diagnostic algorithm for classification and characterization of direct antiglobulin test-negative autoimmune hemolytic anemia.

Research Project

Project/Area Number 18K07421
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 52010:General internal medicine-related
Research InstitutionJichi Medical University

Principal Investigator

Kamesaki Toyomi  自治医科大学, 医学部, 客員研究員 (90316513)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2018: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Keywords自己免疫性溶血性貧血 / クームス試験
Outline of Final Research Achievements

Five to ten percent of patients with autoimmune hemolytic anemia (AIHA) have a negative finding on the direct antiglobulin test (DAT) and are difficult to diagnose. Most of these AIHA patients have amounts of red blood cell-associated IgG below the cut-off value of DAT. Comprehensive diagnosis and classification of DAT-negative AIHA can be made with additional tests of low-affinity IgG and IgA/IgM autoantibodies. However, 17% of patients with DAT-negative AIHA show negativity on all the above tests and are diagnosed with ‘clinically diagnosed DAT-negative AIHA’ following exclusion of other hemolytic anemias and responsiveness to steroids. This percentage could decrease to 4% if tests were done before treatments. Patients with this AIHA show relatively worse prognosis than patients with low-affinity IgG. When treating a patient with DAT-negative hemolysis, DAT-negative AIHA should be considered and tested in reference laboratories, especially before treatment.

Academic Significance and Societal Importance of the Research Achievements

診断に苦慮することが多いクームス陰性AIHAの網羅的診断・分類法を確立し、それぞれの特徴やわが国における頻度を明らかにした。本検査法は、現在、わが国の臨床の場で活用されている。また、クームス陰性AIHAの17%に網羅的診断法では検出できない比較的予後不良な ‘臨床的に診断されたクームス陰性AIHA’ が存在することを明らかにした。機序の解明や直接的な検査法については今後の課題であるが、ステロイド治療前に検査を行うことで4%に減少することから、DAT陰性の溶血貧血患者を診る際には、クームス陰性AIHAも考慮して、治療前に検査を行うことを推奨している。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (56 results)

All 2022 2021 2020 2019 2018 Other

All Journal Article (26 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 1 results) Presentation (15 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results) Book (14 results) Remarks (1 results)

  • [Journal Article] Development of measurement method for autoantibodies against red blood cells by flow cytometric analysis2022

    • Author(s)
      大峠 ふくみ、山城 安啓、緒方 静、木本 真史、森 健太郎、服部 幸夫、亀崎 豊実
    • Journal Title

      Japanese Journal of Medical Technology

      Volume: 71 Issue: 1 Pages: 37-44

    • DOI

      10.14932/jamt.21-17

    • NAID

      130008144359

    • ISSN
      0915-8669, 2188-5346
    • Year and Date
      2022-01-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diagnostic algorithm for classification and characterization of direct antiglobulin test‐negative autoimmune hemolytic anemia with 1‐year clinical follow‐up2022

    • Author(s)
      Kamesaki Toyomi
    • Journal Title

      Transfusion

      Volume: 62 Issue: 1 Pages: 205-216

    • DOI

      10.1111/trf.16709

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diagnosis and classification of direct antiglobulin test-negative autoimmune hemolytic anemia2021

    • Author(s)
      亀崎 豊実
    • Journal Title

      Rinsho Ketsueki

      Volume: 62 Issue: 5 Pages: 456-462

    • DOI

      10.11406/rinketsu.62.456

    • NAID

      130008049774

    • ISSN
      0485-1439, 1882-0824
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 先天性溶血性貧血2021

    • Author(s)
      亀崎豊実
    • Journal Title

      日本医事新報

      Volume: 5055 Pages: 33-34

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 溶血性貧血の診断と検査2021

    • Author(s)
      亀崎豊実
    • Journal Title

      検査と技術

      Volume: 49 Pages: 880-887

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 寒冷凝集素症の臨床病態2021

    • Author(s)
      亀崎豊実
    • Journal Title

      血液内科

      Volume: 82 Pages: 711-716

    • NAID

      40022600478

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 特集 小児の貧血 7.自己免疫性溶血性貧血の診断と治療2020

    • Author(s)
      亀崎 豊実
    • Journal Title

      小児科

      Volume: 61 Issue: 11 Pages: 1511-1520

    • DOI

      10.18888/sh.0000001510

    • ISSN
      0037-4121
    • Year and Date
      2020-10-01
    • Related Report
      2020 Research-status Report
  • [Journal Article] 特集 検査値を読む2020 17章 炎症マーカー検査 ハプトグロビン2020

    • Author(s)
      亀崎 豊実
    • Journal Title

      内科

      Volume: 125 Issue: 4 Pages: 813-814

    • DOI

      10.15106/j_naika125_813

    • ISSN
      0022-1961, 2432-9452
    • Year and Date
      2020-04-01
    • Related Report
      2020 Research-status Report
  • [Journal Article] Demographic characteristics, thromboembolism risk, and treatment patterns for patients with cold agglutinin disease in Japan2020

    • Author(s)
      Kamesaki Toyomi、Nishimura Jun-ichi、Wada Hideho、Yu Eric、Tsao Elisa、Morales Jaime、Kanakura Yuzuru
    • Journal Title

      International Journal of Hematology

      Volume: 112 Issue: 3 Pages: 307-315

    • DOI

      10.1007/s12185-020-02899-6

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 特集 貧血の薬物治療戦略 溶血性貧血2020

    • Author(s)
      亀崎 豊実
    • Journal Title

      薬局

      Volume: 71 Pages: 3112-3118

    • Related Report
      2020 Research-status Report
  • [Journal Article] Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.2020

    • Author(s)
      Kitao A, Kawamoto S, Kurata K, Hayakawa I, Yamasaki T, Matsuoka H, Sumi Y, Kakeji Y, Kamesaki T, Minami H.
    • Journal Title

      Int J Hematol

      Volume: - Issue: 5 Pages: 657-666

    • DOI

      10.1007/s12185-020-02831-y

    • NAID

      120006884211

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 溶血性貧血の診断2019

    • Author(s)
      亀崎豊実
    • Journal Title

      診断と治療

      Volume: 107 Pages: 586-589

    • Related Report
      2019 Research-status Report
  • [Journal Article] 自己免疫性溶血性貧血2019

    • Author(s)
      亀崎豊実
    • Journal Title

      ドクターサロン

      Volume: 63 Pages: 736-740

    • NAID

      10031123095

    • Related Report
      2019 Research-status Report
  • [Journal Article] Progress in diagnosis and treatment of autoimmune hemolytic anemia2019

    • Author(s)
      亀崎豊実
    • Journal Title

      Rinsho Ketsueki

      Volume: 60 Issue: 9 Pages: 1100-1107

    • DOI

      10.11406/rinketsu.60.1100

    • NAID

      130007724584

    • ISSN
      0485-1439, 1882-0824
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 貧血のはなし2019

    • Author(s)
      亀崎豊実
    • Journal Title

      災害補償

      Volume: 562 Pages: 82-83

    • Related Report
      2019 Research-status Report
  • [Journal Article] Ectopic expression of band 3 anion transport protein in colorectal cancer revealed in an autoimmune hemolytic anemia patient2019

    • Author(s)
      Kawamoto Shinichiro、Kamesaki Toyomi、Masutani Ryota、Kitao Akihito、Hatanaka Kazuo、Imakita Masami、Tamaki Toshiharu、Takubo Takayuki
    • Journal Title

      Human Pathology

      Volume: 83 Pages: 193-198

    • DOI

      10.1016/j.humpath.2018.07.020

    • NAID

      120006599107

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 自己免疫性溶血性貧血(AIHA)診療の現状と展望2019

    • Author(s)
      亀崎豊実
    • Journal Title

      Pharma Medica

      Volume: 37 Pages: 69-77

    • Related Report
      2018 Research-status Report
  • [Journal Article] Coombs試験,寒冷凝集素反応などの赤血球抗体検査の読み方2019

    • Author(s)
      亀崎豊実
    • Journal Title

      血液内科

      Volume: 78 Pages: 164-169

    • Related Report
      2018 Research-status Report
  • [Journal Article] 高齢者の溶血性貧血2019

    • Author(s)
      亀崎豊実
    • Journal Title

      血液フロンティア

      Volume: 29 Pages: 42-53

    • Related Report
      2018 Research-status Report
  • [Journal Article] MYELODYSPLASTIC SYNDROME COMPLICATED BY DIRECT ANTIGLOBULIN TEST NEGATIVE HEMOLYSIS2018

    • Author(s)
      Fukahori Michitaka、Yamaguchi Maki、Kamesaki Toyomi
    • Journal Title

      Japanese Journal of Transfusion and Cell Therapy

      Volume: 64 Issue: 3 Pages: 534-539

    • DOI

      10.3925/jjtc.64.534

    • NAID

      130007404562

    • ISSN
      1881-3011, 1883-0625
    • Year and Date
      2018-06-30
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Comprehensive Diagnostic Algorithm for Direct Antiglobulin Test-Negative Autoimmune Hemolytic Anemia Reveals the Relative Ratio of Three Mechanisms in a Single Laboratory2018

    • Author(s)
      Kamesaki Toyomi、Kajii Eiji
    • Journal Title

      Acta Haematologica

      Volume: 140 Issue: 1 Pages: 10-17

    • DOI

      10.1159/000488753

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 貧血の読み方・捉え方 実践編:貧血検査データの読み方 症例③2018

    • Author(s)
      亀崎豊実
    • Journal Title

      Medical Technoplogy

      Volume: 46 Pages: 1075-1079

    • Related Report
      2018 Research-status Report
  • [Journal Article] 自己免疫性溶血性貧血患者,不規則抗体陽性患者への輸血2018

    • Author(s)
      亀崎豊実
    • Journal Title

      内科

      Volume: 122 Pages: 799-803

    • Related Report
      2018 Research-status Report
  • [Journal Article] 直接抗グロブリン試験陽性化の機序と臨床2018

    • Author(s)
      亀崎豊実
    • Journal Title

      血液事業

      Volume: 41

    • Related Report
      2018 Research-status Report
  • [Journal Article] 私の処方2018血液疾患の処方 溶血性貧血2018

    • Author(s)
      亀崎豊実
    • Journal Title

      小児科臨床

      Volume: 71 Pages: 271-278

    • Related Report
      2018 Research-status Report
  • [Journal Article] 自己免疫性溶血性貧血の診断と治療ー最新の展開2018

    • Author(s)
      亀崎豊実
    • Journal Title

      血液内科

      Volume: 76 Pages: 51-58

    • Related Report
      2018 Research-status Report
  • [Presentation] Progress in diagnosis and classification of autoimmune hemolytic anemia. Symposium 2. Hemolytic Anemia: Molecular Pathogenesis and New Treatment.2020

    • Author(s)
      Kamesaki T
    • Organizer
      第82回日本血液学会学術集会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 福山臨床検査センターにおけるフローサイトメトリーによる赤血球結合IgG定量の現状2020

    • Author(s)
      緒方静, 木本真史, 清水頌子, 森健太郎, 早崎俊成, 奥原俊彦, 山城安啓, 大峠ふくみ, 服部幸夫, 亀崎豊実
    • Organizer
      第21回日本検査血液学会学術集会
    • Related Report
      2020 Research-status Report
  • [Presentation] Two pediatric cases of direct anti-globulin test-negative autoimmune hemolytic anemia2019

    • Author(s)
      Moriya K, Matsuki T, Niizuma H, Rikiishi T, Onuma i, Sato A, Sasahara Y, Kamesaki T, Kure S, Imaizumi M
    • Organizer
      第61回日本小児血液・がん学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] 自己免疫性溶血性貧血 ―ガイドライン改定及び血栓塞栓症リスク―2019

    • Author(s)
      亀崎豊実
    • Organizer
      第81回日本血液学会学術集会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 自己免疫性溶血性貧血の診療 ―最近の動向―2019

    • Author(s)
      亀崎豊実
    • Organizer
      第81回日本血液学会学術集会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Seasonal analysis of thromboembolic events in patients with cold agglutinin disease (CAD) in Japan2019

    • Author(s)
      Nishimura J,Kamesaki T,Wada H,Yu E, Morales J,Kanakura Y.
    • Organizer
      第81回日本血液学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Establishment of measurement for autoantibodies on RBC by flow cytometry.2019

    • Author(s)
      Ogata S,Kimoto M,Mori K,Shimizu S, Yamashiro Y,Kamesaki T,Hattori Y
    • Organizer
      第81回日本血液学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] A case of paroxymal nocturnal hemoglobinuria with positive direct Coombs test2019

    • Author(s)
      Takahata A,Bando K,Sonokawa S,Ishida S, Nakamura T,Shimizu T,Kamesaki T,Yamamoto K
    • Organizer
      A case of paroxymal nocturnal hemoglobinuria with positive direct Coombs test
    • Related Report
      2019 Research-status Report
  • [Presentation] Ectopic band 3 expression stimulates autoantibody production causing colorectal cancer-related anemia2019

    • Author(s)
      北尾章人, 川本晋一郎, 倉田啓史, 早川郁代, 山崎隆, 松岡広, 角泰雄, 掛地吉弘, 亀崎豊実, 南博信
    • Organizer
      第17回日本臨床腫瘍学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] CLINICAL CHARACTERISTICS, TREATMENT PATTERNS, AND THROMBOEMBOLIC RISK OF PATIENTS WITH COLD AGGLUTININ DISEASE (CAD) IN JAPAN2019

    • Author(s)
      Nishimura J, Kamesaki T, Wada H, Yu E, Tsao E, Morales J, Kanakura Y
    • Organizer
      European Hematology Association 24(June 14, 2019, Amsterdam)
    • Related Report
      2019 Research-status Report
  • [Presentation] Establishment of the measurement of autoantibodies against red blood cells by flow cytometry2018

    • Author(s)
      大峠ふくみ, 山城安啓, メラフェラニア, 天尾優希, 緒方静, 木本真史, 森健太郎, 亀崎豊実, 服部幸夫
    • Organizer
      第80回日本血液学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Flow cytometric measurement of erythrocyte membrane-bound IgG: A potential diagnostic method for colorectal carcinoma2018

    • Author(s)
      北尾章人, 川本晋一郎, 早川郁代, 倉田啓史, 山崎隆, 松岡広, 角泰雄, 掛地吉弘, 亀崎豊実, 南博信
    • Organizer
      第16回日本臨牀腫瘍学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Flow cytometric measurement of increase in erythrocyte-bound IgG: A potential diagnostic method for colorectal carcinoma2018

    • Author(s)
      Akihito Kitao, Shinichiro Kawamoto, Keiji Kurata, Ikuyo Hayakawa, Hiroshi Matsuoka, Yasuo Sumi, Yoshihiro Kakeji, Toyomi Kamesaki, Hironobu Minam
    • Organizer
      AACR Annual Meeting 2018 in Chicag
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 著明な赤血球自己凝集を伴う温式自己免疫性溶血性貧血を合併した骨髄異形成症候群2018

    • Author(s)
      森拓人, 小野祐一郎, 楠本寿子, 石川隆之, 亀崎豊実
    • Organizer
      第80回日本血液学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 溶血性貧血の基礎と病型診断に関わる臨床検査のポイント2018

    • Author(s)
      亀崎豊実
    • Organizer
      第19回日本検査血液学会学術集会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Book] 専門医のための血液病学2022

    • Author(s)
      鈴木 隆浩、竹中 克斗、池添 隆之
    • Total Pages
      432
    • Publisher
      医学書院
    • ISBN
      9784260047722
    • Related Report
      2021 Annual Research Report
  • [Book] 今日の治療指針 2022年版[デスク判]2022

    • Author(s)
      福井 次矢、高木 誠、小室 一成
    • Total Pages
      2224
    • Publisher
      医学書院
    • ISBN
      9784260047760
    • Related Report
      2021 Annual Research Report
  • [Book] EBM血液疾患の治療2021-2022(寒冷凝集素症(CAD)に対する治療と開発中の新規治療薬)2020

    • Author(s)
      亀崎豊実(金倉譲監修)
    • Total Pages
      637
    • Publisher
      中外医学社
    • ISBN
      9784498225244
    • Related Report
      2020 Research-status Report
  • [Book] 免疫・炎症疾患のすべて (日本医師会生涯教育シリーズ) (自己免疫性溶血性貧血(AIHA))2020

    • Author(s)
      亀崎豊実(竹内勤監修)
    • Total Pages
      366
    • Publisher
      診断と治療社
    • ISBN
      9784787824776
    • Related Report
      2020 Research-status Report
  • [Book] 新臨床内科学第10版 (酵素異常による遺伝性溶血性貧血 自己免疫性溶血性貧血)2020

    • Author(s)
      亀崎豊実(矢崎義雄監修)
    • Total Pages
      1960
    • Publisher
      医学書院
    • ISBN
      9784260038065
    • Related Report
      2020 Research-status Report
  • [Book] 今日の診断指針第8版 (自己免疫性溶血性貧血)2020

    • Author(s)
      亀崎豊実(永井良三監修)
    • Total Pages
      2114
    • Publisher
      医学書院
    • ISBN
      9784260038089
    • Related Report
      2020 Research-status Report
  • [Book] 自己免疫性溶血性貧血診療の参照ガイド(令和1年改訂版)2020

    • Author(s)
      張替秀郎, 西村純一, 植田康敬, 高森弘之, 和田秀穂, 亀崎豊実, 川本晋一郎, 池添隆之, 菅野仁, 大賀正一, 服部幸夫, 山城安啓, 金倉譲
    • Total Pages
      44
    • Publisher
      厚生労働科学研究費補助金 難治性疾患等政策研究事業 特発性造血障害に関する調査研究班(研究代表者 三谷絹子)
    • Related Report
      2020 Research-status Report
  • [Book] 指定難病ペディア2019 (日本医師会生涯教育シリーズ)2019

    • Author(s)
      亀崎豊実(水澤英洋、五十嵐隆、北川泰久、高橋和久、弓倉整 監修・編集)
    • Total Pages
      368
    • Publisher
      診断と治療社
    • ISBN
      4787824163
    • Related Report
      2019 Research-status Report
  • [Book] 私の治療[2019-20年度版]2019

    • Author(s)
      亀崎豊実(監修 猿田享男,北村惣一郎),
    • Total Pages
      1568
    • Publisher
      日本医事新報社
    • ISBN
      9784784946518
    • Related Report
      2019 Research-status Report
  • [Book] 血液専門医テキスト(改訂第3版)2019

    • Author(s)
      亀崎豊実(日本血液学会編)
    • Total Pages
      660
    • Publisher
      南江堂
    • ISBN
      9784524248827
    • Related Report
      2019 Research-status Report
  • [Book] 未来型血液治療学2019

    • Author(s)
      亀崎豊実(小松則夫編)
    • Total Pages
      254
    • Publisher
      中外医学社
    • ISBN
      9784498225183
    • Related Report
      2019 Research-status Report
  • [Book] 血液疾患最新の治療2020-20222019

    • Author(s)
      亀崎豊実(中尾、松村、神田編)
    • Total Pages
      390
    • Publisher
      南江堂
    • ISBN
      9784524249787
    • Related Report
      2019 Research-status Report
  • [Book] 自己免疫性溶血性貧血神領の参照ガイド平成28年度改訂版2019

    • Author(s)
      金倉譲、亀崎豊実、梶井英治、川本晋一郎、北尾章人、萩原浩平、鈴木隆浩、唐沢正光、小峰光博
    • Total Pages
      253
    • Publisher
      特発性造血障害に関する調査研究班
    • Related Report
      2019 Research-status Report
  • [Book] 今日の治療指針 2019年版[デスク判]2019

    • Author(s)
      亀崎豊実(福井 次矢編集)
    • Total Pages
      2160
    • Publisher
      医学書院
    • Related Report
      2018 Research-status Report
  • [Remarks] 自己免疫性溶血性貧血(AIHA) に関する検査

    • URL

      http://aiha.a.la9.jp/AIHA/index.html

    • Related Report
      2020 Research-status Report 2019 Research-status Report 2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi