Processing of crude drugs - The mechanism of the appearance of immunostimulatory activity by heating with honey
| Project/Area Number |
18K07453
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52010:General internal medicine-related
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| Research Institution | Nagoya City University |
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Principal Investigator |
Ota Misato 名古屋市立大学, 大学院薬学研究科, 研究員 (00767121)
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| Co-Investigator(Kenkyū-buntansha) |
牧野 利明 名古屋市立大学, 医薬学総合研究院(薬学), 教授 (80326561)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 蜂蜜 / イソマルトース / 顆粒球コロニー刺激因子 / 腸管免疫 / トール様受容体 / 蜜炙 / isomaltose / 加熱加工 / 高分子 / TLR4 / 加熱 / G-CSF / 免疫賦活作用 / 生薬 / 修治 |
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| Outline of Final Research Achievements |
This study aimed to obtain the scientific evidence on the processing process of roasting crude drugs with honey (honey-roasting). Various honey market products and various sugars contained in honey were used as experimental materials, which were heated under various conditions. Murine gastrointestinal epithelial cells (MCE301 cells) were cultured with these samples, and the concentration of G-CSF (granulocyte-colony stimulating factor) released into the medium was measured by the ELISA. Inducible activity of G-CSF production was appeared in various honey samples by heat processing. From the correlation between the content of sugars and the activity, it was discovered that isomaltose contributes to this activity, and the maximum conditions were 180° C for 1 hour and 200 °C for 15 to 30 minutes. Isomaltose contributed to the induction of G-CSF production in heated honey, and it is suggested that honey with a high isomaltose content is suitable for honey roasting.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では蜂蜜を加熱するだけで、免疫賦活作用が得られることを発見し、それを担う含有成分としてisomaltoseを得た。本研究成果から、バラツキの大きい蜂蜜市場品の品質を評価するときの指標としてisomaltose含量が提案でき、商品の差別化に応用できる内容である。
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Report
(4 results)
Research Products
(9 results)
