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Using RNAi techniques, practical use for control of brain edema with aquaporin regulation

Research Project

Project/Area Number 18K08835
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 55050:Anesthesiology-related
Research InstitutionAichi Medical University

Principal Investigator

Yoshihito Fujita  愛知医科大学, 医学部, 教授 (90238593)

Co-Investigator(Kenkyū-buntansha) 浅井 清文  名古屋市立大学, 医薬学総合研究院(医学), 教授 (70212462)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords脳浮腫 / プレコンディショニング / 低酸素 / 脳保護 / ノックダウン / アクアポリン / AQP4 / 脳低温
Outline of Final Research Achievements

We developed that acquaporin knockdown cell lines and over-expression cell lines and we also demonstrated that bupivacaine induces neural death with calcium channel type T of bupivacaine.
We confirmed that with the specific incubator that could make accurate low O2 condition, our cell lines could make grow in 1% O2 condition and that means that hypoxic preconditioning could be established. Moreover, we combined this 1% O2 hypoxic condition and low glucose level and we established neural ischemic models. We could proceed nest steps for our experiments.

Academic Significance and Societal Importance of the Research Achievements

低温による脳浮腫軽減は明らかであるが、臨床での大規模スタディーでは効果が否定されることが多い。AQP発現変化が脳低温の脳保護効果の一翼を担っている可能性が示唆される。本研究では、AQPのknockdownやoverexpressionでの脳浮腫や細胞機能への影響を検討している。また、低酸素となる状況下では、プレコンディショニングが行われている。その状況から、遺伝子操作や薬物、低温を使用し、神経細胞へのダメージを最小限にする方法を模索したい。プレコンディショニングの確立はさらなる研究へのステップとなる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (30 results)

All 2022 2020 2019 2018

All Journal Article (8 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 8 results,  Open Access: 8 results) Presentation (22 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results)

  • [Journal Article] Effect of intensive care provided by nurse practitioners for postoperative patients: a retrospective observational before-and -after study.2022

    • Author(s)
      Kazunao Mori, Satoshi Makino, Takuya Takabayashi, Masahiro Kurosawa, Wataru Ohashi, Yoko Tsukamoto, Masatoshi Okumura, Yoshihito Fujita and Yoshihiro Fujiwara.
    • Journal Title

      PLoS One

      Volume: 17 Issue: 1 Pages: 1-1

    • DOI

      10.1371/journal.pone.0262605

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Antimicrobial de-escalation in the critically ill patient and assessment of clinical cure: the DIANA study.2020

    • Author(s)
      Liesbet De Bus, Pieter Depuydt, et al. and DIANA study group (including Yoshihito Fujita)
    • Journal Title

      Intensive Care Med

      Volume: 46 Issue: 7 Pages: 1404-17

    • DOI

      10.1007/s00134-020-06111-5

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Anesthetic management of a patient with congenital insensitivity to pain with anhidrosis by coadministration of remifentanil2018

    • Author(s)
      Takeuchi Yoko、Fujita Yoshihito、Shimomura Takeshi、Kurokawa Shuji、Noguchi Hiroki、Fujiwara Yoshihiro
    • Journal Title

      JA Clinical Reports

      Volume: 4 Issue: 1 Pages: 72-72

    • DOI

      10.1186/s40981-018-0208-8

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficacy and safety of sugammadex in patients undergoing renal transplantation2018

    • Author(s)
      Ono Yasumasa、Fujita Yoshihito、Kajiura Takahiro、Okawa Hazuki、Nakashima Juntaro、Isobe Hideo、Fujiwara Yoshihiro
    • Journal Title

      JA Clinical Reports

      Volume: 4 Issue: 1 Pages: 56-56

    • DOI

      10.1186/s40981-018-0192-z

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of maternal low-glucose during Cesarean section neonatal hypoglycemia2018

    • Author(s)
      1.Saya Yoshizawa, Yoshihito Fujita, Maiko Hoshika, Nobuyoshi Kusama, Kazuya Sobue
    • Journal Title

      J Anesthesiol Cli Sci.

      Volume: 7 Issue: 1 Pages: 2-2

    • DOI

      10.7243/2049-9752-7-2

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of 2- or 4-hour oral intake restriction on intraoperative intravascular volume optimization using stroke volume variation analysis: A single-blinded randomized control trial.2018

    • Author(s)
      2.Maiko Hoshika, Yoshihito Fujita, Saya Yoshizawa, Megumi Harima, Kazuya Sobue.
    • Journal Title

      Korean J Anesthesiol

      Volume: 71 Issue: 3 Pages: 239-241

    • DOI

      10.4097/kja.d.17.00012

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 気道確保が必要となった甲状腺穿刺吸引細胞診後の一過性甲状腺腫大の症例2018

    • Author(s)
      橋本 篤、木下浩之、磯部英男、榊原健介、藤田義人、畠山 登、藤原祥裕
    • Journal Title

      日集中医誌

      Volume: 25 Pages: 195-6

    • NAID

      130006731115

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] チェックリストを用いたブリーフィングの導入と、早期リハビリテーションに与える影響2018

    • Author(s)
      磯部英男、藤田義人
    • Journal Title

      日救急医会誌

      Volume: 29 Pages: 142-5

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Prognostic value of procalcitonin after elective non-cardiac surgery: a cohort study.2022

    • Author(s)
      Y. Fujita and Y. Fujiwara.
    • Organizer
      The first Joint Scientific Congress of TSCCM, TSECCM, and JSICM
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of intraoperative magnesium on postoperative RASS score after endovascular repair of aortic aneurysm: a preliminary randomized controlled trial.2020

    • Author(s)
      R Jyoko, Y. Fujita, H. Kanamori, R. Ishihara, Y. Fujiwara
    • Organizer
      Annual Meeting of the European Anesthesiology (ESA)
    • Related Report
      2020 Research-status Report
  • [Presentation] 腹腔鏡下拡大右半結腸切除術後に腹腔内出血を来したダビガトラン内服患者にイダルシズマブを投与した1例2020

    • Author(s)
      石原亮太、藤田義人、磯部英男、橋本篤、佐藤祐子、藤原祥裕
    • Organizer
      第47回日本集中治療医学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] アドレナリンはβ2受容体の活性化を介してLPSによって誘導されたマクロファージにおける炎症反応を制御する2020

    • Author(s)
      王聡、馮国剛、石原亮太、橋本篤、奥村将年、佐藤祐子、藤田義人、藤原祥裕
    • Organizer
      第47回日本集中治療医学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] イオン化マグネシウムの臨床での有用性について 教育セミナー(ランチョン)2020

    • Author(s)
      藤田義人
    • Organizer
      第47回日本集中治療医学会学術集会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Prognostic value of procalcitonin after gynecological surgery: a preliminary retrospective cohort study.2019

    • Author(s)
      Y. Fujita , R. Ishihara , A. Hashimoto , Y. Sato , M. Okumura , T. Shimomura , Y. Fujiwara.
    • Organizer
      2019 Annual congress of the European Society of Intensive Care Medicine (ESICM) in Berlin
    • Related Report
      2019 Research-status Report
  • [Presentation] Perioperative management of two patients with anti-NMDA receptor encephalitis, including a 13-year-old adolescent.2019

    • Author(s)
      Y Hattori, Y Fujita, Y. Fujiwara.
    • Organizer
      2019 Annual Meeting of the European Anesthesiology (ESA) in Wien
    • Related Report
      2019 Research-status Report
  • [Presentation] 周術期における感染対策 【K02】(感染対策)専門医共通講習2019

    • Author(s)
      藤田義人
    • Organizer
      第17回日本麻酔科学会東海・北陸支部学術集会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 当院RSTによる院内全死亡例におけるバイタルサイン、特に呼吸回数、SpO2測定の頻度および異常認識調査2019

    • Author(s)
      岩田純子、藤田義人、RSTチーム
    • Organizer
      第40回日本呼吸療法医学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] A病院呼吸サポートチーム(RST)による院内全死亡例におけるバイタルサインの変容調査 ~呼吸回数は本当に重症化を予測できるのか?~ (優秀演題)2019

    • Author(s)
      吉田有紀子、藤田義人、RSTチーム
    • Organizer
      第3回日本集中治療医学会東海北陸支部学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] MEPモニター使用時でも完全静脈麻酔 (TIVA)にこだわる必要はない2019

    • Author(s)
      黒川修二、藤田義人
    • Organizer
      第23回日本神経麻酔集中治療学会
    • Related Report
      2019 Research-status Report
  • [Presentation] MEPモニター使用時でも完全静脈麻酔 (TIVA)にこだわる必要はない2019

    • Author(s)
      黒川修二、藤田義人
    • Organizer
      第23回日本神経麻酔集中治療学会 奈良
    • Related Report
      2018 Research-status Report
  • [Presentation] プロプラノロールはLPSで誘導されるTNF-α mRNA発現量に及ぼすエピネフリンの効果に拮抗する2019

    • Author(s)
      杉浦春香、藤原祥裕、馮 国剛、藤田義人、橋本 篤、榊原健介、田中久美子、奥村将年、磯部英男、石原亮太
    • Organizer
      第46回日本集中治療医学会学術集会 京都
    • Related Report
      2018 Research-status Report
  • [Presentation] 一時的大静脈フィルターを抜去した当日に肺血栓塞栓症を起こした一例2019

    • Author(s)
      中島淳太郎、榊原健介、奥村将年、橋本 篤、下村 毅、藤田義人、藤原祥裕
    • Organizer
      第46回日本集中治療医学会学術集会 京都
    • Related Report
      2018 Research-status Report
  • [Presentation] エクリズマブに抵抗性を示した痙攣重積発作が初発症状であった非典型型尿毒症症候群の一症例2019

    • Author(s)
      磯部英男、奥村将年、榊原健介、橋本 篤、下村 毅、藤田義人、畠山 登、藤原祥裕
    • Organizer
      第46回日本集中治療医学会学術集会 京都
    • Related Report
      2018 Research-status Report
  • [Presentation] Lack of association between neutrophil-to-lymphocyte ratio (NLR) and ICU stay in patients with sepsis; a retrospective cohort study.2018

    • Author(s)
      Y. Fujita , R. Ishihara , A. Hashimoto , Y. Sato , M. Okumura , T. Shimomura , Y. Fujiwara.
    • Organizer
      2018 Annual congress of the European Society of Intensive Care Medicine (ESICM) in Paris, France
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The effect of epinephrine and propranolol on the expression of tumor necrosis factor-alfa mRNA induced by lipopolysaccharide in RAW 264.7 cells.2018

    • Author(s)
      Y Fujiwara, G-G Feng, K Tanaka, R Ishihara, N Hatakeyama, A Hashimoto, Y Fujita.
    • Organizer
      2018 Annual congress of the European Society of Intensive Care Medicine (ESICM) in Paris, France
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Anesthetic management of a patient with congenital insensitivity to pain and anhidrosis using remifentanil.2018

    • Author(s)
      Takeuchi Y, Fujita Y, Shimomura T, Kurokawa S, Noguchi H, Fujiwara Y.
    • Organizer
      2018 Annual Meeting of the European Anesthesiology (ESA) in Copenhagen, Denmark.
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Hypothermia during general anesthesia was associated with the postoperative delirium after endovascular repair of aortic aneurysm.2018

    • Author(s)
      Hashimoto A, Ishihara R, Fujita Y, Fujiwara Y.
    • Organizer
      2018 Annual Meeting of the European Anesthesiology (ESA) in Copenhagen, Denmark.
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 食道がんの気管内浸潤にて人工呼吸に難渋した1例(優秀演題)2018

    • Author(s)
      恒川亜里沙、藤田義人、藤原祥裕
    • Organizer
      第16回日本麻酔科学会東海・北陸支部学術集会 金沢
    • Related Report
      2018 Research-status Report
  • [Presentation] 生体腎移植患者におけるロクロニウム/スガマデクス使用の有用性と安全性 ―術後長期間フォローでの検討―2018

    • Author(s)
      大野泰昌、藤田義人、藤原祥裕
    • Organizer
      日本麻酔科学会第65回学術集会 横浜
    • Related Report
      2018 Research-status Report
  • [Presentation] 抗利尿ホルモン不適合分泌症候群と塩類喪失性腎症とが併存したシスプラチンによる低ナトリウム血症の一例2018

    • Author(s)
      下村毅、磯部英男、奧村将年、榊原健介、橋下篤、藤田義人、畠山登、藤原祥裕
    • Organizer
      第45回日本集中治療医学会学術集会 幕張
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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