| Project/Area Number |
18K09834
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57070:Developmental dentistry-related
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| Research Institution | The University of Tokushima |
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Principal Investigator |
UEDA Kimiko 徳島大学, 病院, 助教 (40335807)
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| Co-Investigator(Kenkyū-buntansha) |
岩本 勉 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (90346916)
北村 尚正 徳島大学, 大学院医歯薬学研究部(歯学域), 助教 (50614020)
赤澤 友基 徳島大学, 病院, 助教 (10646152)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 硬組織形成 / 超遠心法 / ウシ胎児血清 / 歯原性上皮細胞 / 歯原性間葉細胞 / 増殖 / 分化 / 超遠心 / 細胞分化 |
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| Outline of Final Research Achievements |
Focusing on the fraction and its components of the fetal bovine serum (FBS) extracted by ultracentrifugation, the effect on hard tissue formation cells was examined. As a result, the first fraction promoted differentiation and the third fraction promoted proliferation. Therefore, proteomic analysis was performed, and the characteristic proteins of each fraction were confirmed in comparison with the controls. From the results, we focused on several molecules and examined about them. It was suggested that Fam3C is one of a factor that promotes the differentiation of odontogenic mesenchymal cells. In the future, we would like to elucidate the mechanisms about them and aim for the research that will lead to the development of new treatment methods for dentin regeneration or bone regeneration as practical research.
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| Academic Significance and Societal Importance of the Research Achievements |
歯の形成には、永久歯ではおよそ 10 年から 15 年と、長い期間を必要とする。今日の歯に関する研究の進歩にも関わらず、歯の再生技術の実用化が難しい原因の一つに、歯の形成には長期の時間がかかることが考えられる。すなわち、硬組織の形成を促進する因子やその詳細な機構については不明な点が多い。そこで FBS に含まれる硬組織形成促進因子が同定され、その機構が明らかになれば、硬組織形成細胞の増殖・分化の促進が可能となる。特に、これまで長い時間を要した石灰化についての研究で、今後の同研究を期間短縮という点で大きく後押しし、これまで不明だった様々な分子メカニズムの解明に大きく貢献できると考えられた。
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