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Development of motile sperm sorting system by control of sperm motility

Research Project

Project/Area Number 18K12069
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 90110:Biomedical engineering-related
Research InstitutionOkayama University of Science

Principal Investigator

MATSUURA Koji  岡山理科大学, 工学部, 准教授 (70443223)

Co-Investigator(Kenkyū-buntansha) 高田 耕児  富山県産業技術研究開発センター, その他部局等, 主任研究員 (40530621)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords精子運動性 / マイクロデバイス / 酸化還元指示薬 / ヒト精子 / 運動精子 / ATP / 解糖系 / 温度勾配 / マイクロ流体デバイス / 酸化的リン酸化
Outline of Final Research Achievements

We used a particle size sorting microfluidic chip, and could not motile sperm sorting using the microfluidic chip due to low hydrodynamic diameter of motile sperm. We also tried to develop a microfluidic device containing a dissolving film to recover motile sperm by taxis and temperature control system for utilizing themotaxis of sperm. However, these prototype devices did not work well. To evaluate motility control mechanism in mammalian sperm, we found expression of enzymes related to glycolysis in human sperm. Some results using paper-based microfluidic device with redox indicator suggests the relationship between the redox reactivities of the indicators and of related redox enzymes in the sperms. In future, we will evaluate relationship between motility and enzyme reactivity for energy production in sperm cells, and develop a condition that mammalian sperms can maintain their motility for several hours.

Academic Significance and Societal Importance of the Research Achievements

運動精子回収数を増加するデバイスの開発を進めているが、今回用いたマイクロデバイスではその回収数を著しく増加できるような効果が得られず、別の原理を検討すべきと判断した。
精子運動活性の試験紙デバイスを用いた評価については、試薬と精子の反応とメカニズムについて検証する方向性が定まった。精子のエネルギー輸送に着目して、精子運動に関する酵素活性の評価方法が発見されれば、自然な受精や人工授精の成否が以前よりも高い確率で予測できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (2 results)

  • [Int'l Joint Research] 台湾国立清華大学(その他の国・地域)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 国立清華大学(その他の国・地域)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] 国立台湾清華大学(その他の国・地域・台湾)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Point-of-care semen analysis of patients with infertility via smartphone and colorimetric paper-based diagnostic device2020

    • Author(s)
      Yu-Ting Tsao, Chung-Yao Yang, Yun-Chiao Wen, Ting-Chang Chang, Koji Matsuura, Yu Chen, Chao-Min Cheng
    • Journal Title

      Bioengineering & Translational Medicine

      Volume: 6 Issue: 1

    • DOI

      10.1002/btm2.10176

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Numerical Study of the Lift Force, Velocities and Pressure Distribution of a Single Air Bubble and Two Interacting Air Bubbles Rising in Quiescent Liquid2020

    • Author(s)
      Chao Guan, Shinichiro Yanase, Koji Matsuura, Toshinori Kouchi, and Yasunori Nagata
    • Journal Title

      Open Journal of Fluid Dynamics

      Volume: 10 Issue: 01 Pages: 31-51

    • DOI

      10.4236/ojfd.2020.101003

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spiral Trajectory Modulation of Rheotaxic Motile Human Sperm in Cylindrical Microfluidic Channels of Different Inner Diameters2019

    • Author(s)
      Saori Nishina, Koji Matsuura, and Keiji Naruse
    • Journal Title

      Acta Medica Okayama

      Volume: 73 Issue: 3 Pages: 213-221

    • DOI

      10.18926/AMO/56863

    • NAID

      120006647999

    • URL

      https://ousar.lib.okayama-u.ac.jp/56863

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Paper-Based Resazurin Assay of Inhibitor-Treated Porcine Sperm2019

    • Author(s)
      Koji Matsuura, Wei-Hsin Wang, Alex Ching, Yu Chen, and Chao-Min Cheng
    • Journal Title

      Micromachines

      Volume: 10 Issue: 8 Pages: 495-495

    • DOI

      10.3390/mi10080495

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Spiral trajectory modulation in rheotaxic motile human sperm in cylindrical microfluidic channels of different inner diameters2019

    • Author(s)
      Saori Nishina, Koji Matsuura, Keiji Naruse
    • Journal Title

      Acta Medica Okayama

      Volume: -

    • NAID

      120006647999

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 培養細胞の色素取り込み評価に関する検討2020

    • Author(s)
      松浦 宏冶, 濵田 将吾
    • Organizer
      CHEMINAS42
    • Related Report
      2020 Annual Research Report
  • [Presentation] Hydrostatic pressure threshold for the reduction of human sperm motility2018

    • Author(s)
      Noriki Tanaka, Saori Nishina, Ayano Fujita, Masatoshi Morimatsu, Yuka Asano, Koji Matsuura, Keiji Naruse
    • Organizer
      The 56th Annual Meeting of The Biophysical Society of Japan
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-08-18  

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