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Molecular analysis of persulfide dependent signaling involved in bioactive regulation

Research Project

Project/Area Number 18K14650
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 43030:Functional biochemistry-related
Research InstitutionThe University of Tokyo

Principal Investigator

Shimizu Takayuki  東京大学, 大学院総合文化研究科, 助教 (90817214)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsシグナル制御 / システイン修飾 / 硫化水素 / パースルフィド / 細菌
Outline of Final Research Achievements

Hydrogen sulfide modulates important physiological processes and molecular species containing polysulfur that are called reactive sulfur species (RSS) are the actual signaling molecules. However, RSS signaling and metabolism are not fully understood. To elucidate this, I investigated biochemical properties of RSS sensor protein and RSS metabolic pathways. I revealed that the RSS sensor protein predominantly reacts with specific RSS. Moreover, RSS metabolic enzyme which is related to generation of its RSS was also identified. These results are important to understand the underlying mechanisms of RSS signaling.

Academic Significance and Societal Importance of the Research Achievements

活性イオウ分子種を介したシグナル伝達系の分子基盤の一端を世界で始めた明らかにした本研究成果は、硫化水素・活性イオウ分子種に依存したシグナル伝達の詳細と、その生理的意義を明らかにする上で極めて重要である。また、硫化水素は、脳疾患である統合失調症や心臓疾患である心不全などの様々な疾患に関与することが報告されているため、様々な疾患に対するこれまでにない全く新しいアプローチ・治療法の開発につながることが期待される。したがって、本理学研究の成果は、医学・薬学研究にも大きく波及すると言える。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (14 results)

All 2021 2020 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Repressor Activity of SqrR, a Master Regulator of Persulfide-Responsive Genes, Is Regulated by Heme Coordination2021

    • Author(s)
      Shimizu T, Hayashi Y, Arai M, McGlynn SE, Masuda T, Masuda S
    • Journal Title

      Plant Cell Physiol

      Volume: 62 Issue: 1 Pages: 100-110

    • DOI

      10.1093/pcp/pcaa144

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Role of Tetrapyrrole- and GUN1-Dependent Signaling on Chloroplast Biogenesis2021

    • Author(s)
      Shimizu T, MasudaT
    • Journal Title

      Plants

      Volume: 10 Issue: 2 Pages: 196-196

    • DOI

      10.3390/plants10020196

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae2020

    • Author(s)
      Takayuki Shimizu, Rintaro Yasuda, Yui Mukai, Ryo Tanoue, Tomohiro Shimada, Sousuke Imamura, Kan Tanaka, Satoru Watanabe and Tatsuru Masuda
    • Journal Title

      Philosophical Transactions of the Royal Society B

      Volume: 375 Issue: 1801 Pages: 20190488-20190488

    • DOI

      10.1098/rstb.2019.0488

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Persulphide-responsive transcriptional regulation and metabolism in bacteria2019

    • Author(s)
      Shimizu Takayuki、Masuda Shinji
    • Journal Title

      The Journal of Biochemistry

      Volume: 167 Pages: 125-132

    • DOI

      10.1093/jb/mvz063

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis.2019

    • Author(s)
      Shimizu, T., Kacprzak, S.M., Mochizuki, N., Nagatani, A., Watanabe, S., Shimada, T., Tanaka, K., Hayashi, Y., Arai, M., Leister, D., Okamoto, H., Terry, M.J., & Masuda, T.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 116 Issue: 49 Pages: 24900-24906

    • DOI

      10.1073/pnas.1911251116

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 硫化水素依存的な生理活性調節におけるパースルフィド応答機構の分子基盤2021

    • Author(s)
      清水隆之、増田真二、増田建
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 葉緑体形成の制御に関わる葉緑体から核へのシグナル伝達2020

    • Author(s)
      清水隆之
    • Organizer
      光合成学会オンラインミニシンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 硫化水素による生理活性調節とそのシグナル伝達機構2020

    • Author(s)
      清水隆之
    • Organizer
      静岡大学招待講演
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 硫化水素による生理活性調節におけるパースルフィド検知機構の分子基盤2020

    • Author(s)
      清水隆之、増田真二、増田建
    • Organizer
      第61回日本植物生理学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 硫化水素依存的な光合成の制御因子SqrRを起点としたパースルフィド代謝経路の解析2019

    • Author(s)
      清水隆之、増田建、増田真二
    • Organizer
      第10回日本光合成学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 生理活性物質としての硫化水素のシグナル伝達機構に関与するパースルフィド代謝系の解析2019

    • Author(s)
      清水隆之、増田建、増田真二
    • Organizer
      第60回日本植物生理学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 硫化水素依存的な光合成の制御因子SqrRはヘム応答性転写因子として機能する2018

    • Author(s)
      清水隆之、増田建、増田真二
    • Organizer
      第9回日本光合成学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Persulfide-responsive transcriptional repressor SqrR regulates sulfide-dependent photosynthesis2018

    • Author(s)
      Takayuki Shimizu, Jiangchuan Shen, Mingxu Fang, Yixiang Zhang, Koichi Hori, Jonathan C. Trinidad, Carl E. Bauer, David P. Giedroc and Shinji Masuda
    • Organizer
      18th International Symposium of Phototrophic Prokaryotes
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Persulfide-responsive transcriptional repressor SqrR regulates sulfide-dependent photosynthesis2018

    • Author(s)
      Takayuki Shimizu, Jiangchuan Shen, Mingxu Fang, Yixiang Zhang, Koichi Hori, Jonathan C. Trinidad, Carl E. Bauer, David P. Giedroc and Shinji Masuda
    • Organizer
      1st Asia-Oceania International Congress on Photosynthesis
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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