| Project/Area Number |
18K17977
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 59040:Nutrition science and health science-related
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | システイン / シスチン / マクロファージ / 酸化ＬＤＬ / 動脈硬化 |
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| Outline of Final Research Achievements |
Phagocytosis of oxidized LDL by macrophages is an important process in the development of atherosclerosis. Cystine, a metabolite of cysteine, enhanced the uptake of oxidized LDL by macrophages (in vitro). Macrophages treated with reagents inducing ER stress showed reduced uptake of oxidized LDL, whereas this was not the case in the presence of cystine (in vitro). These results suggest that macrophages that have taken up oxidized LDL might have reduced the capacity for oxidized LDL uptake due to ER stress, whereas macrophages that have taken up oxidized LDL might maintain their ability to uptake oxidized LDL in the presence of cystine. In vivo, however, no difference in uptake of oxidized LDL was observed in macrophages from cystine-fed mice. We would like to examine the relationship between ER stress and cystine in vivo in the near future.
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| Academic Significance and Societal Importance of the Research Achievements |
アミノ酸の一種であるシステインは、色素沈着や二日酔いの改善などのサプリメントとして広く利用されている。In vitroの結果からは、システインの代謝物であるシスチンが酸化LDLによるERストレスを抑えることでマクロファージの酸化LDL取込みを促進する可能性が示唆された。今回、in vivoの結果からはシスチンが動脈硬化を増悪させるデータは得られなかった。しかし、ERストレスが生じている環境下では、シスチンが動脈硬化を増悪させる可能性は否定できない。また、過剰なシステイン摂取が糖尿病を悪化させる可能性が報告されていることを考慮すると、適度なサプリメント摂取が望ましいと考える。
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