Immune modulation by a symbiotic bacterium Alcaligenes in gut associated lymphoid tissues

• Project/Area Number: 18K17997
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: National Institutes of Biomedical Innovation, Health and Nutrition
• Principal Investigator: Hosomi Koji 国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 ワクチン・アジュバント研究センター, 主任研究員 (00755762)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 腸内細菌 / 共生 / 免疫 / 樹状細胞 / 一酸化窒素 / 代謝 / ミトコンドリア / LPS / 共生細菌 / パイエル板 / ワクチン / アジュバント / 粘膜免疫 / リピドA / アルカリゲネス / 腸管免疫 / アポトーシス / LPS活性 / リンパ組織内共生 / エネルギー代謝

Outline of Final Research Achievements

We examined the interaction between the intestinal lymphoid tissue resident commensal bacteria and immune cells, and clarified the symbiotic mechanism at the cellular and molecular levels. Alcaligenes, a symbiotic bacterium inside Peyer's patches (PPs), promotes the production of cytokines that enhance IgA antibody production from dendritic cells (DCs), but hardly induces the generation of nitric oxide, which causes inflammation. Thus, it is considered that Alcaligenes contributes to the activation of appropriate immune responses while maintaining immunological homeostasis. Furthermore, Alcaligenes may establish a sustainable symbiotic relationship within PPs through increasing mitochondrial activity and suppressing apoptotic cell death in DCs.

Academic Significance and Societal Importance of the Research Achievements

アルカリゲネスの免疫学的ユニーク性の一端は、本菌のリポ多糖（LPS）やその活性中心であるリピドAの特殊な構造と免疫学的性状によって説明できる。そこで、適度な免疫活性化作用をもつアルカリゲネスLPSやリピドAを活用したワクチンアジュバントの開発を進め、皮下や経鼻ワクチンに対して有効性と安全性のバランスに優れたアジュバント効果を発揮することを明らかにした。現在、実験用アジュバントとして販売され、また、ワクチン・製薬メーカーと共同で実用化に向けた検討を進めており、本研究で得られた学術知見を基盤として、アジュバント開発を目指した応用研究へ発展しており、社会的にも意義のある研究である。

Research Products

• [Journal Article] Relationships between barley consumption and gut microbiome characteristics in a healthy Japanese population: a cross-sectional study (2022)
  • Author(s): Matsuoka Tsubasa、Hosomi Koji、Park Jonguk、Goto Yuka、Nishimura Mao、Maruyama Satoko、Murakami Haruka、Konishi Kana、Miyachi Motohiko、Kawashima Hitoshi、Mizuguchi Kenji、Kobayashi Toshiki、Yokomichi Hiroshi、Kunisawa Jun、Yamagata Zentaro
  • Journal Title: BMC Nutrition Volume: 8 Issue: 1 Pages: 23-23
  • DOI: 10.1186/s40795-022-00500-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Gut Microbiome as a Biomarker of Cancer Progression Among Female Never-smokers With Lung Adenocarcinoma (2022)
  • Author(s): OTOSHI TAKEHIRO、NAGANO TATSUYA、PARK JONGUK、HOSOMI KOJI、YAMASHITA TOMOYA、TACHIHARA MOTOKO、TABATA TOKIKO、SEKIYA REINA、TANAKA YUGO、KOBAYASHI KAZUYUKI、MIZUGUCHI KENJI、ITOH TOMOO、MANIWA YOSHIMASA、KUNISAWA JUN、NISHIMURA YOSHIHIRO
  • Journal Title: Anticancer Research Volume: 42 Issue: 3 Pages: 1589-1598
  • DOI: 10.21873/anticanres.15633
  • Peer Reviewed / Open Access
• [Journal Article] Categorization of the Ocular Microbiome in Japanese Stevens?Johnson Syndrome Patients With Severe Ocular Complications (2021)
  • Author(s): Ueta Mayumi、Hosomi Koji、Park Jonguk、Mizuguchi Kenji、Sotozono Chie、Kinoshita Shigeru、Kunisawa Jun
  • Journal Title: Frontiers in Cellular and Infection Microbiology Volume: 11 Pages: 741654-741654
  • DOI: 10.3389/fcimb.2021.741654
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chemically Synthesized Alcaligenes Lipid A as an Adjuvant to Augment Immune Responses to Haemophilus Influenzae Type B Conjugate Vaccine (2021)
  • Author(s): Liu Zilai、Hosomi Koji、Shimoyama Atsushi、Yoshii Ken、Sun Xiao、Lan Huangwenxian、Wang Yunru、Yamaura Haruki、Kenneth Davie、Saika Azusa、Nagatake Takahiro、Kiyono Hiroshi、Fukase Koichi、Kunisawa Jun
  • Journal Title: Frontiers in Pharmacology Volume: 12 Pages: 763657-763657
  • DOI: 10.3389/fphar.2021.763657
  • Peer Reviewed / Open Access
• [Journal Article] Lipopolysaccharide Derived From the Lymphoid-Resident Commensal Bacteria Alcaligenes faecalis Functions as an Effective Nasal Adjuvant to Augment IgA Antibody and Th17 Cell Responses (2021)
  • Author(s): Wang Yunru、Hosomi Koji、Shimoyama Atsushi、Yoshii Ken、Nagatake Takahiro、Fujimoto Yukari、Kiyono Hiroshi、Fukase Koichi、Kunisawa Jun
  • Journal Title: Frontiers in Immunology Volume: 12 Pages: 699349-699349
  • DOI: 10.3389/fimmu.2021.699349
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comprehensive analysis of gut microbiota of a healthy population and covariates affecting microbial variation in two large Japanese cohorts (2021)
  • Author(s): Park J, Kato K, Murakami H, Hosomi K, Tanisawa K, Nakagata T, Ohno H, Konishi K, Kawashima H, Chen YA, Mohsen A, Xiao JZ, Odamaki T, Kunisawa J, Mizuguchi K, Miyachi M
  • Journal Title: BMC Microbiol Volume: 21 Issue: 1 Pages: 151-151
  • DOI: 10.1186/s12866-021-02215-0
  • Peer Reviewed / Open Access
• [Journal Article] 12-Hydroxyeicosapentaenoic acid inhibits foam cell formation and ameliorates high-fat diet-induced pathology of atherosclerosis in mice (2021)
  • Author(s): Nagatake T, Shibata Y, Morimoto S, Node E, Sawane K, Hirata SI, Adachi J, Abe Y, Isoyama J, Saika A, Hosomi K, Tomonaga T, and Kunisawa J.
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 10426-10426
  • DOI: 10.1038/s41598-021-89707-1
  • Peer Reviewed / Open Access
• [Journal Article] ω3 fatty acid metabolite, 12-hydroxyeicosapentaenoic acid, alleviates contact hypersensitivity by downregulation of CXCL1 and CXCL2 gene expression in keratinocytes via retinoid X receptor α (2021)
  • Author(s): Saika, A., Nagatake, T., Hirata, S.I., Sawane, K., Adachi, J., Abe, Y., Isoyama, J., Morimoto, S., Node, E., Tiwari, P., Hosomi, K., Matsunaga, A., Honda, T., Tomonaga, T., Arita, M., Kabashima, K., and Kunisawa, J.
  • Journal Title: FASEB J Volume: 35 Issue: 4
  • DOI: 10.1096/fj.202001687r
  • Peer Reviewed / Open Access
• [Journal Article] Lipopolysaccharide from Gut‐Associated Lymphoid‐Tissue‐Resident Alcaligenes faecalis : Complete Structure Determination and Chemical Synthesis of Its Lipid?A (2021)
  • Author(s): Shimoyama A、Di Lorenzo F、Yamaura H、Mizote K、Palmigiano A、Pither MD.、Speciale I、Uto T、Masui S、Sturiale L、Garozzo D、Hosomi K、Shibata N、Kabayama K、Fujimoto Y、Silipo A、Kunisawa Jun、Kiyono H、Molinaro A、Fukase K
  • Journal Title: Angewandte Chemie International Edition Volume: 60 Issue: 18 Pages: 10023-10031
  • DOI: 10.1002/anie.202012374
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lymphoid Tissue Resident Alcaligenes Establish an Intracellular Symbiotic Environment by Creating a Unique Energy Shift in Dendritic Cells (2020)
  • Author(s): Hosomi Koji、Shibata Naoko、Shimoyama Atsushi、Uto Tomoya、Nagatake Takahiro、Tojima Yoko、Nishino Tomomi、Takeyama Haruko、Fukase Koichi、Kiyono Hiroshi、Kunisawa Jun
  • Journal Title: Frontiers in Microbiology Volume: 11 Pages: 561005-561005
  • DOI: 10.3389/fmicb.2020.561005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of the intestinal environment on the immune responses to vaccination (2020)
  • Author(s): Hosomi Koji、Kunisawa Jun
  • Journal Title: Vaccine Volume: 38 Issue: 44 Pages: 6959-6965
  • DOI: 10.1016/j.vaccine.2020.08.079
  • Peer Reviewed / Open Access
• [Journal Article] Chemically Synthesized Alcaligenes Lipid A Shows a Potent and Safe Nasal Vaccine Adjuvant Activity for the Induction of Streptococcus pneumoniae-Specific IgA and Th17 Mediated Protective Immunity (2020)
  • Author(s): Yoshii Ken、Hosomi Koji、Shimoyama Atsushi、Wang Yunru、Yamaura Haruki、Nagatake Takahiro、Suzuki Hidehiko、Lan Huangwenxian、Kiyono Hiroshi、Fukase Koichi、Kunisawa Jun
  • Journal Title: Microorganisms Volume: 8 Issue: 8 Pages: 1102-1102
  • DOI: 10.3390/microorganisms8081102
  • Peer Reviewed / Open Access
• [Journal Article] Adjuvant Activity of Synthetic Lipid A of Alcaligenes, a Gut-Associated Lymphoid Tissue-Resident Commensal Bacterium, to Augment Antigen-Specific IgG and Th17 Responses in Systemic Vaccine (2020)
  • Author(s): Wang Yunru、Hosomi Koji、Shimoyama Atsushi、Yoshii Ken、Yamaura Haruki、Nagatake Takahiro、Nishino Tomomi、Kiyono Hiroshi、Fukase Koichi、Kunisawa Jun
  • Journal Title: Vaccines Volume: 8 Issue: 3 Pages: 395-395
  • DOI: 10.3390/vaccines8030395
  • Peer Reviewed / Open Access
• [Journal Article] Impaired Mucociliary Motility Enhances Antigen-Specific Nasal IgA Immune Responses to a Cholera Toxin-Based Nasal Vaccine (2020)
  • Author(s): Lan H, Suzuki H, Nagatake T, Hosomi K, Ikegami K, Setou M, Kunisawa J.
  • Journal Title: Int Immunol Volume: in press Issue: 8 Pages: 559-568
  • DOI: 10.1093/intimm/dxaa029
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diversity of Energy Metabolism in Immune Responses Regulated by Microorganisms and Dietary Nutrition (2020)
  • Author(s): Hosomi K, Kunisawa J
  • Journal Title: Int Immunol Volume: in press Issue: 7 Pages: 447-454
  • DOI: 10.1093/intimm/dxaa020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of the oral adsorbent AST-120 on fecal p-cresol and indole levels and on the gut microbiota composition (2020)
  • Author(s): Emiko Sato, Koji Hosomi, Akiyo Sekimoto, Eikan Mishim, Yuji Oe, Daisuke Saigusa, Sadayoshi Ito, Takaaki Abe, Hiroshi Sato, Jun Kunisawa, Toshimitsu Niwa, Nobuyuki Takahashi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 525 Issue: 3 Pages: 773
  • DOI: 10.1016/j.bbrc.2020.02.141
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Maternal omega-3 docosapentaenoic acid inhibits infant allergic dermatitis through TRAIL-expressing plasmacytoid dendritic cells in mice (2020)
  • Author(s): Hirata SI, Nagatake T, Sawane K, Hosomi K, Honda T, Ono S, Shibuya N, Saito E, Adachi J, Abe Y, Isoyama J, Suzuki H, Matsunaga A, Tomonaga T, Kiyono H, Kabashima K, Arita M, and Kunisawa J
  • Journal Title: Allergy Volume: - Issue: 8 Pages: 0-0
  • DOI: 10.1111/all.14217
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Persistent colonization of non-lymphoid tissue-resident macrophages by Stenotrophomonas maltophilia (2019)
  • Author(s): Takahashi Ichiro, Hosomi Koji, Nagatake Takahiro, Tobou Hirokazu, Yamamoto Daiki, Hayashi Ikue, Kurashima Yosuke, Sato Shintaro, Shibata Naoko, Goto Yoshiyuki, Maruyama Fumito, Nakagawa Ichiro, Kuwae Asaomi, Abe Akio, Kunisawa Jun, Kiyono Hiroshi
  • Journal Title: International Immunology Volume: 32 Issue: 2 Pages: 133-141
  • DOI: 10.1093/intimm/dxz071
  • NAID: 120006888456
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 17( S),18( R)-epoxyeicosatetraenoic Acid Generated by Cytochrome P450 BM-3 From Bacillus megaterium Inhibits the Development of Contact Hypersensitivity via G-protein-coupled Receptor 40-mediated Neutrophil Suppression (2019)
  • Author(s): Saika A, Nagatake T, Kishino S, Park SB, Honda T, Matsumoto N, Shimojou M, Morimoto S, Tiwari P, Node E, Hirata SI, Hosomi K, Kabashima K, Ogawa J, Kunisawa J.
  • Journal Title: FASEB Bioadv . Volume: 2 Pages: 59-59
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dietary omega-3 fatty acid dampens allergic rhinitis via eosinophilic production of the anti-allergic lipid mediator 15-hydroxyeicosapentaenoic acid in mice (2019)
  • Author(s): Sawane K, Nagatake T, Hosomi K, Hirata SI, Adachi J, Abe Y, Isoyama J, Suzuki H, Matsunaga A, Fukumitsu S, Aida K, Tomonaga T, Arita M, and Kunisawa J
  • Journal Title: Nutrients Volume: 11 Issue: 12 Pages: 2868-2868
  • DOI: 10.3390/nu11122868
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clostridium Perfringens Enterotoxin-Based Protein Engineering for the Vaccine Design and Delivery System (2019)
  • Author(s): Lan H, Hosomi K, Kunisawa J.
  • Journal Title: Vaccine Volume: 37 Issue: 42 Pages: 6232-6232
  • DOI: 10.1016/j.vaccine.2019.08.032
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] BLT1 Mediates Commensal Bacteria-Dependent Innate Immune Signals to Enhance Antigen-Specific Intestinal IgA Responses (2019)
  • Author(s): Nagatake T, Hirata SI, Koga T, Kuroda E, Kobari S, Suzuki H, Hosomi K, Matsumoto N, Yanrismet Y, Shimojou M, Morimoto S, Sasaki F, Ishii KJ, Yokomizo T, Kunisawa J.
  • Journal Title: Mucosal Immunol . Volume: 12 Issue: 5 Pages: 1082-1082
  • DOI: 10.1038/s41385-019-0175-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fatty Acid Metabolism in the Host and Commensal Bacteria for the Control of Intestinal Immune Responses and Diseases (2019)
  • Author(s): Hosomi K, Kiyono H, Kunisawa J.
  • Journal Title: Gut Microbes Volume: 23 Issue: 3 Pages: 1-1
  • DOI: 10.1080/19490976.2019.1612662
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Analysis of Oral Microbiota in Japanese Oral Cancer Patients Using 16S rRNA Sequencing (2019)
  • Author(s): Takahashi Y, Park J, Hosomi K, Yamada T, Kobayashi A, Yamaguchi Y, Iketani S, Kunisawa J, Mizuguchi K, Maeda N, Ohshima T.
  • Journal Title: J Oral Biosci . Volume: 61 Issue: 2 Pages: 120-120
  • DOI: 10.1016/j.job.2019.03.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Metabolism of Dietary and Microbial Vitamin B Family in the Regulation of Host Immunity (2019)
  • Author(s): Yoshii Ken、Hosomi Koji、Sawane Kento、Kunisawa Jun
  • Journal Title: Frontiers in Nutrition Volume: 6 Pages: 48-48
  • DOI: 10.3389/fnut.2019.00048
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of Adjuvant-Free Bivalent Food Poisoning Vaccine by Augmenting the Antigenicity of Clostridium perfringens Enterotoxin (2018)
  • Author(s): Suzuki Hidehiko、Hosomi Koji、Nasu Ayaka、Kondoh Masuo、Kunisawa Jun
  • Journal Title: Frontiers in Immunology Volume: 9 Pages: 2320-2320
  • DOI: 10.3389/fimmu.2018.02320
  • Peer Reviewed / Open Access
• [Journal Article] Development of a bivalent food poisoning vaccine: augmented antigenicity of the C-terminus of Clostridium perfringens enterotoxin by fusion with the B subunit of Escherichia coli Shiga toxin 2 (2018)
  • Author(s): Hosomi Koji、Hinenoya Atsushi、Suzuki Hidehiko、Nagatake Takahiro、Nishino Tomomi、Tojima Yoko、Hirata So-ichiro、Matsunaga Ayu、Kondoh Masuo、Yamasaki Shinji、Kunisawa Jun
  • Journal Title: International Immunology Volume: 31 Issue: 2 Pages: 91-100
  • DOI: 10.1093/intimm/dxy071
  • Peer Reviewed
• [Journal Article] Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice. (2018)
  • Author(s): Nagatake T, Suzuki H, Hirata S, Matsumoto N, Wada Y, Morimoto S, Nasu A, Shimojou M, Kawano M, Ogami K, Tsujimura Y, Kuroda E, Iijima N, Hosomi K, Ishii KJ, Nosaka T, Yasutomi Y, Kunisawa J.
  • Journal Title: Int Immunol Volume: 30 Issue: 10 Pages: 471-481
  • DOI: 10.1093/intimm/dxy046
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immunostimulatory effect on dendritic cells of the adjuvant-active exopolysaccharide from Leuconostoc mesenteroides strain NTM048 (2018)
  • Author(s): Matsuzaki C, Takagaki C, Higashimura Y, Nakashima Y, Hosomi K, Kunisawa J, Yamamoto K, Hisa K.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 82 Issue: 9 Pages: 1647-1651
  • DOI: 10.1080/09168451.2018.1482195
  • Peer Reviewed
• [Journal Article] Lymphoid tissue-resident Alcaligenes LPS induces IgA production without excessive inflammatory responses via weak TLR4 agonist activity (2018)
  • Author(s): N. Shibata, J. Kunisawa, K. Hosomi, Y. Fujimoto, K. Mizote, N. Kitayama, A. Shimoyama, H. Mimuro, S. Sato, N. 5 Kishishita, K. J. Ishii, K. Fukase, and H. Kiyono
  • Journal Title: Mucosal Immunology Volume: － Issue: 3 Pages: 693-702
  • DOI: 10.1038/mi.2017.103
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] パイエル板組織内共生菌アルカリゲネスのリポ多糖の免疫学的特徴を利用したアジュバント開発 (2021)
  • Author(s): 細見晃司、下山敦史、柴田納央子、王韻茹、吉井健、長竹貴広、宇戸智哉、山浦遼生、藤本ゆかり、清野宏、深瀬浩一、國澤純
  • Organizer: 第25回日本ワクチン学会学術集会
• [Presentation] A symbiotic mechanism of intestinal lymphoid tissue resident Alcaligenes by controlling metabolic modification in dendritic cells. (2021)
  • Author(s): Koji Hosomi, Takahiro Nagatake, Hiroshi Kiyono, Jun Kunisawa
  • Organizer: 第50回日本免疫学会学術集会
• [Presentation] Effective and safe adjuvant activity of Alcaligenes LPS and its synthetic lipid A for nasal vaccine. (2021)
  • Author(s): Koji Hosomi1, Yunru Wang, Ken Yoshii, Atsushi Shimoyama, Takahiro Nagatake, Azusa Saika, Tomoya Uto, Haruki Yamaura, Davie Kenneth, Yukari Fujimoto, Hiroshi Kiyono, Koichi Fukase, Jun Kunisawa
  • Organizer: 16th Meeting of the International Endotoxin and Innate Immunity Society
  • Int'l Joint Research
• [Presentation] 小腸パイエル板組織内共生菌アルカリゲネスと樹状細胞の相互作用 (2020)
  • Author(s): 細見晃司，柴田納央子，下山敦史，宇戸智哉，長竹貴広，東島陽子，西野友美，竹山春子，深瀬浩一，清野宏，國澤純
  • Organizer: 腸内細菌学会
• [Presentation] Chemically synthesized Alcaligenes lipid A as a new systemic and mucosal vaccine adjuvant (2019)
  • Author(s): Koji Hosomi, Atsushi Shimoyama, Wang Yunru, Ken Yoshii, Hiroshi Kiyono, Koichi Fukase, Jun Kunisawa
  • Organizer: ASM Microbe 2019
  • Int'l Joint Research
• [Presentation] Chemically synthesized Alcaligenes lipid A as a specifically Th17 response-inducing vaccine adjuvant (2019)
  • Author(s): Koji Hosomi, Yunru Wang, Ken Yoshii, Atsushi Shimoyama, Hiroshi Kiyono, Koichi Fukase, Jun Kunisawa
  • Organizer: ISV 2019 Annual Congress
  • Int'l Joint Research
• [Presentation] 小腸パイエル板組織内共生細菌アルカリゲネスの免疫機能とアジュバント応用 (2019)
  • Author(s): 柴田納央子、細見晃司、下山敦史、王韻茹、吉井健、深瀬浩一、清野宏、國澤純
  • Organizer: 第15回日本小児消化管感染症研究会
  • Invited
• [Presentation] Development of a bivalent food poisoning vaccine: augmented antigenicity of C-terminal region of Clostridium perfringensenterotoxin by fusion with Escherichia coli Shiga toxin 2 B subunit (2018)
  • Author(s): Hosomi K, Hinenoya A, Suzuki H, Nagatake T, Nishino T, Toshima Y, Kondoh M, Yamasaki S, Kunisawa J
  • Organizer: 第16回松山国際学術シンポジウム&若手の会
  • Int'l Joint Research
• [Presentation] Development of a bivalent vaccine for Clostridium perfringens enterotoxin and Escherichia coli Shiga toxin 2 (2018)
  • Author(s): Koji Hosomi and Jun Kunisawa
  • Organizer: 2nd Korea-Japan joint meeting on mucosal immunology
  • Int'l Joint Research
• [Presentation] Augmentation of antigenicity of the C-terminal region of Clostridium perfringens enterotoxin by fusion with the B subunit of Escherichia coli Shiga toxin 2 for the development of bivalent food poisoning vaccine (2018)
  • Author(s): Koji Hosomi, Atsushi Hinenoya, Hidehiko Suzuki, Takahiro Nagatake, Tomomi Nishino, So-ichiro Hirata, Masuo Kondoh, Shinji Yamasaki, Jun Kunisawa
  • Organizer: International Society for Vaccines
  • Int'l Joint Research
• [Presentation] ウェルシュ菌および志賀毒素産生性大腸菌感染症に対する多価食中毒ワクチンの開発 (2018)
  • Author(s): 細見晃司、日根野谷淳、鈴木英彦、長竹貴広、西野友美、東島陽子、近藤昌夫、山崎伸二、國澤純
  • Organizer: 日本ワクチン学会
• [Book] 食と腸内細菌から考える健康長寿最前線 (2022)
  • Author(s): 細見晃司、國澤純
  • Publisher: アンチ・エイジング医学
• [Book] 粘膜面における獲得免疫と感染防御 (2021)
  • Author(s): 細見晃司、國澤純
  • Total Pages: 6
  • Publisher: 医学のあゆみ
• [Book] 腸内細菌の代謝物を介した免疫機能制御 (2021)
  • Author(s): 吉井健、細見晃司、國澤純
  • Total Pages: 11
  • Publisher: 腸内細菌学雑誌
• [Book] 栄養学と腸内細菌から考える個別化医療の将来展望 (2019)
  • Author(s): 細見晃司、國澤純
  • Total Pages: 4
  • Publisher: Precision Medicine
• [Book] 油と腸内フローラから考える健康科学の新展開 (2019)
  • Author(s): 細見晃司、國澤純
  • Total Pages: 8
  • Publisher: イルシー
• [Book] ヒトマイクロバイオームビッグデータと健康医療への応用 (2018)
  • Author(s): 細見晃司、國澤純
  • Total Pages: 6
  • Publisher: 月刊化学工業
• [Book] 腸内環境に関連するビッグデータの集積と創薬、機能性食品開発への展望. 腸内細菌叢を標的にした医薬品と保健機能食品の開発 (2018)
  • Author(s): 細見晃司、國澤純
  • Total Pages: 7
  • Publisher: 技術情報協会