Constructing artificial neuronal circuits on diamond and long-term monitoring of the spontaneous firing pattern with NV centers

• Project/Area Number: 18K19026
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 29:Applied condensed matter physics and related fields
• Research Institution: Waseda University
• Principal Investigator: TANII Takashi 早稲田大学, 理工学術院, 教授 (20339708)
• Project Period (FY): 2018-06-29 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 神経細胞回路 / 自発発火 / NVセンター / ダイヤモンド / 細胞パターニング / NVセンタ / 神経細胞 / ＮＶセンタ / 光検出磁気共鳴法 / 自発発火パターン

Outline of Final Research Achievements

There are two approaches to understand an extremely complex system such as the brain; namely, top-down approaches which resolve the whole system into building blocks and bottom-up approaches to construct the whole system from scratch. In this work, we aimed at (1) developing a method to detect and monitor neural spikes by combining cell-patterning techniques with NV centers in diamond for a long term and (2) understanding the spontaneous firing patterns experimentally and theoretically by constructing neuronal circuits containing only a single or a few neurons on the substrate surface.

Academic Significance and Societal Importance of the Research Achievements

ダイヤモンド中のNVセンターを電位プローブとして実神経細胞の発火を検出できる計測系の実現を試みると同時に，そのようにして基板上の構築された実神経細胞回路が提示するであろう発火パターンの理論予測の両面から研究を進めた。現時点では，NVセンターによる実神経細胞の発火計測は，当初の予測に反し，感度不足が原因で達成されていない。一方，単一または少数個の実細胞からなる神経細胞回路が発現する自発発火パターンに関する理論予測において，特にオータプスが発現する機能の観点から新たな発見があり，オータプスが神経ネットワーク全体の同期生成に深く関与していることを示唆した。

Research Products

• [Int'l Joint Research] CNR(イタリア)
• [Int'l Joint Research] Ulm University(ドイツ)
• [Int'l Joint Research] CNR(イタリア)
• [Int'l Joint Research] Ulm University(ドイツ)
• [Int'l Joint Research] CNR(イタリア)
• [Journal Article] Selective Stimulation of a Target Neuron in Micropatterned Neuronal Circuits Using a Pair of Needle Electrodes (2021)
  • Author(s): Hattori Kouhei、Kurakake Hekiru、Imai Junko、Hashimoto Takuya、Ishida Mihoko、Sato Koki、Takahashi Honoka、Oguma Soichiro、Yamamoto Hideaki、Hirano-Iwata Ayumi、Tanii Takashi
  • Journal Title: Electrochemistry Volume: 89 Issue: 4 Pages: 348-354
  • DOI: 10.5796/electrochemistry.21-00032
  • NAID: 130008062336
  • ISSN: 1344-3542, 2186-2451
  • Year and Date: 2021-07-05
  • Peer Reviewed / Open Access
• [Journal Article] Role of Noise in Spontaneous Activity of Networks of Neurons on Patterned Silicon Emulated by Noise-activated CMOS Neural Nanoelectronic Circuits (2021)
  • Author(s): Hasani Ramin、Ferrari Giorgio、Yamamoto Hideaki、Tanii Takashi、Prati Enrico
  • Journal Title: Nano Express Volume: － Issue: 2 Pages: 100438-100438
  • DOI: 10.1088/2632-959x/abf2ae
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Contribution of AMPA and NMDA receptors in the spontaneous firing patterns of single neurons in autaptic culture (2020)
  • Author(s): Kouhei Hattori, Takeshi Hayakawa, Akira Nakanishi, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
  • Journal Title: Biosystems Volume: 198 Pages: 104278-104278
  • DOI: 10.1016/j.biosystems.2020.104278
  • Peer Reviewed / Open Access
• [Journal Article] Triple nitrogen-vacancy centre fabrication by C5N4Hn ion implantation (2019)
  • Author(s): M.Haruyama, S.Onoda, T.Higuchi, W.Kada, A.Chiba, Y.Hirano, T.Teraji, R.Igarashi, S.Kawai, H.Kawarada, Y.Ishii, R.Fukuda, T.Tanii, J.Isoya, T.Ohshima, O.Hanaizum
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 2664-2664
  • DOI: 10.1038/s41467-019-10529-x
  • Peer Reviewed / Open Access
• [Journal Article] Nitrogen-Terminated Diamond Surface for Nanoscale NMR by Shallow Nitrogen-Vacancy Centers (2019)
  • Author(s): S. Kawai, H. Yamano, T. Sonoda, K. Kato, J. Buendia, T. Kageura, R. Fukuda, T. Okada, T. Tanii, T. Higuchi, M. Haruyama, K. Yamada, S. Onoda, T. Ohshima, W. Kada, O. Hanaizumi, A. Stacey, T. Teraji, S. Kono, J. Isoya, H. Kawarada
  • Journal Title: J. Phys. Chem. C Volume: 123 Issue: 6 Pages: 3594-3604
  • DOI: 10.1021/acs.jpcc.8b11274
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of modular organization on dynamical richness in cortical networks (2018)
  • Author(s): H. Yamamoto, S. Moriya, K. Ide, T. Hayakawa, H. Akima, S. Sato, S. Kubota, T. Tanii, M. Niwano, S. Teller, J. Soriano, A. Hirano-Iwata
  • Journal Title: Science Advances Volume: 4 Issue: 11
  • DOI: 10.1126/sciadv.aau4914
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] マイクロパターン上の神経回路における標的ニューロンへの二本針電極を用いた選択的刺激 (2021)
  • Author(s): 小熊奏一郎，服部晃平，鞍掛碧流，今井絢子，石田実穂子，髙橋穂乃歌，山本英明，平野愛弓，谷井孝至
  • Organizer: 2021年 第68回応用物理学会春季学術講演会
• [Presentation] Extracellular stimulation of a target neuron in a micropatterned neuronal circuit using a pair of needle electrodes (2020)
  • Author(s): Kouhei Hattori, Mihoko Ishida, Honoka Takahashi, Soichiro Oguma, Takashi Tanii, Hideaki Yamamoto, Ayumi Hirano-Iwata
  • Organizer: The 8th RIEC International Symposium on Brain Functions and Brain Computer, BFBC 2020
  • Int'l Joint Research
• [Presentation] Extracellular stimulation of a target neuron in micro-patterned neuronal circuits using a pair ofneedle electrodes (2020)
  • Author(s): Kouhei Hattori, Hekiru Kurakake, Junko Imai, Takuya Hashimoto, Mihoko Ishida, Koki Sato, Honoka Takahashi, Soichiro Oguma, Hideaki Yamamoto, Ayumi Hirano-Iwata,Takashi Tanii
  • Organizer: PRIME2020
  • Int'l Joint Research
• [Presentation] オータプス伝搬遅延によるSTDPシナプスの淘汰: 孤立した単一神経細胞に関する数理モデル化 (2020)
  • Author(s): 石田実穂子, 中西彬, 服部晃平, 山本英明, 平野愛弓, 谷井孝至
  • Organizer: 日本神経回路学会全国大会(JNNS2020)
• [Presentation] 単一NVセンター規則配列と単一細胞パターニングを用いた神経細胞回路の自発発火パターンの長期計測に向けて (2019)
  • Author(s): 谷井孝至、石田実穂子、石井邑
  • Organizer: 量子生命科学会第1回大会
• [Presentation] Synaptic plasticity in an isolated single autaptic neuron on a micropattern (2019)
  • Author(s): Akira Nakanishi, Kouhei Hattori, Takeshi Hayakawa, Mihoko Ishida, Hekiru Kurakake, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
  • Organizer: 日本神経回路学会 第29回全国大会
• [Presentation] 2本針型刺激電極の試作とマイクロパターン上神経細胞回路への刺激導入に関する実行可能性評価 (2019)
  • Author(s): 服部 晃平、鞍掛 碧流、今井 絢子、橋本 拓弥、佐藤 晃揮、髙橋 穂乃歌、早川 岳志、山本 英明、平野 愛弓、谷井 孝至
  • Organizer: 第80回応用物理学会秋季学術講演会
• [Presentation] Microcontact printing of scaffold proteins for engineering neuronal network functions (2019)
  • Author(s): Hideaki Yamamoto, Satoshi Moriya, Katsuya Ide, Takeshi Hayakawa, Hisanao Akima, Shigeo Sato, Shigeru Kubota, Michio Niwano, Sara Teller, Jordi Soriano, Takashi Tanii, Ayumi Hirano-Iwata
  • Organizer: The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development
  • Int'l Joint Research
• [Presentation] A computational study on the spontaneous firing pattern of single autaptic neurons -The contribution of AMPA and NMDA current- (2019)
  • Author(s): Mihoko Ishida, Kouhei Hattori, Akira Nakanishi, Takeshi Hayakawa, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
  • Organizer: The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development
  • Int'l Joint Research
• [Presentation] An experimental study on the spontaneous firing of single isolated autaptic neurons using micropatterned substrates (2019)
  • Author(s): Honoka Takahashi, Koki Sato, Mihoko Ishida, Soichiro Oguma, Takeshi Hayakawa, Kouhei Hattori, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
  • Organizer: The 4th International Symposium on Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development
  • Int'l Joint Research
• [Presentation] Self-align fabrication of nano-reservoir with NV center in diamond surface for nuclear magnetic resonance of small molecules (2019)
  • Author(s): Kazuto Kawakatsu, Yu Ishii, Yuki Hata, Yuta Saito, Kosuke Nakamura, Kirou Nagaoka, Takahiro Sonoda, Tetsuya, Tatsuishi, Tokuyuki Teraji, Shinobu Onoda, Taisei Higuchi, Keisuke Yamada, Takeshi Oshima, Takahiro Shinada, Hiroshi Kawarada, Wataru Kada, Osamu Hanaizumi, Junichi Isoya and Takashi Tanii
  • Organizer: 32nd International Microprocesses and Nanotechnology Conference
  • Int'l Joint Research
• [Presentation] An experimental study on the spontaneous firing of single isolated neurons in autaptic culture using micropatterned substrates (2019)
  • Author(s): Kouhei Hattori, Koki Sato, Honoka Takahashi, Soichiro Oguma, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
  • Organizer: the 8th RIEC International Symposium on Brain Functions and Brain Computer
  • Int'l Joint Research
• [Presentation] Junko Imai, Hekiru Kurakake, Takuya Hashimoto, Kouhei Hattori, Koki Sato, Honoka Takahashi, Soichiro Oguma, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii (2019)
  • Author(s): Junko Imai, Hekiru Kurakake, Takuya Hashimoto, Kouhei Hattori, Koki Sato, Honoka Takahashi, Soichiro Oguma, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
  • Organizer: the 8th RIEC International Symposium on Brain Functions and Brain Computer
  • Int'l Joint Research
• [Presentation] 高分解能ナノ NMR に向けたダイヤモンドへの NV センターと微小容器の一括形成 (2019)
  • Author(s): 齋藤悠太、石井邑、川勝一斗、永岡希朗、畑雄貴、中村洸介、榎田尊昭、徐海州、薗田隆弘、立石哲也、金久京太郎、寺地徳之、小野田忍、樋口泰成、山田圭介、大島武、品田高宏、川原田洋、加田渉、花泉修、磯谷順一、谷井孝至
  • Organizer: 第67回応用物理学会春季学術講演会
• [Presentation] マイクロパターン上の神経細胞への2針電極による刺激とその応答計測 (2019)
  • Author(s): 鞍掛 碧流, 服部 晃平, 今井 絢子, 橋本 拓弥, 佐藤 晃揮, 髙橋 穂乃歌, 小熊 奏一郎, 石田 実穂子, 山本 英明, 平野 愛弓, 谷井 孝至
  • Organizer: 第67回応用物理学会春季学術講演会
• [Presentation] イオン注入による単一不純物欠陥の規則的配列形成をその応用 (2019)
  • Author(s): 谷井孝至、品田高宏、寺地徳之、小野田忍、大島武、McGuuinness Liam, Jelezko Fedor, Liu Yan, Wu E, 加田渉、花泉修、川原田洋、磯谷順一
  • Organizer: 第66回応用物理学会春季学術講演会
  • Invited
• [Presentation] Surface modification with visible-light-responsible TiO2 thin film (2019)
  • Author(s): Atsushi Kurotobi, Sho Kono, Takayuki Ichikawa, Hideaki Yamamoto, Ayumi Hirano-Iwata and Tanii Takashi
  • Organizer: The 7th RIEC International Symposium on Brain Functions and Brain Computer
  • Int'l Joint Research
• [Presentation] An experimental study on spontaneous firing of a single neuron on micropatterned substrates (2019)
  • Author(s): Koki Sato, Takahiro Nakane, Honoka Takahashi, Kouhei Hattori, Hideaki Yamamoto, Ayumi Hirano-Iwata and Takashi Tanii
  • Organizer: The 7th RIEC International Symposium on Brain Functions and Brain Computer
  • Int'l Joint Research
• [Presentation] Computational modeling of spontaneous firing patterns generated by single autaptic neurons (2019)
  • Author(s): Kouhei. Hattori, Takeshi Hayakawa, Akira Nakanishi, Mihoko Ishida, Hideaki Yamamoto, Ayumi Hirano-Iwata, Takashi Tanii
  • Organizer: The 7th RIEC International Symposium on Brain Functions and Brain Computer
  • Int'l Joint Research
• [Presentation] ダイヤモンド中単一NVセンターのパルス光磁気共鳴測 定のためのローエンドFPGAへのフォトンカウンタの実装 (2019)
  • Author(s): 永岡 希朗, 畑 雄貴, 川勝 一斗, 石井 邑, 福田 諒 介, 寺地 徳之, 小野田 忍, 大島 武, 品田 高宏 , 川 原田 洋, 磯谷 順一, 谷井 孝至
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] 高被覆率窒素終端(111)ダイヤモンドの作製 (2019)
  • Author(s): 第66回応用物理学会春季学術講演会
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] C5N4Hnイオン注入による双極子結合したNVセンターの 形成 (2019)
  • Author(s): 春山 盛善, 小野田 忍, 樋口 泰成, 加田 渉, 千 葉 敦也, 平野 貴美, 寺地 徳之, 五十嵐 龍治, 河合 空, 川原田 洋, 石井 邑, 福田 諒介, 谷井 孝至, 磯谷 順一, 大島 武, 花泉 修
  • Organizer: 第66回応用物理学会春季学術講演会