Moleculer mechanisms of endogenous voltage-sensitive enzyme in Xenopus oocytes
Project/Area Number |
18K19428
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 48:Biomedical structure and function and related fields
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2018-06-29 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | 膜電位 / イノシトールリン脂質 / アフリカツメガエル |
Outline of Final Research Achievements |
We studied endogenous activity of voltage-sensitive phosphoinositide enzyme in oocytes of Xenopus laevis by electrophysiological measurements and voltage-clamp fluorometry of phosphoinositide-sensitive fluorescent probes with reference to newt oocytes which do not exhibit endogenous voltage-sensitive phosphoinositide enzyme activity. Findings indicate that signal transduction of this innate activity in Xenopus oocyte differs from previously established mechanisms including voltage-sensing phosphatases where voltage sensor domain tightly couples with PTEN-like phosphatase within a single molecule, thereby supporting a possibility that some novel voltage-sensitive molecule exists in Xenopus oocyte.
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Academic Significance and Societal Importance of the Research Achievements |
生物において電気信号を化学信号に変換する分子機構は従来知られてきた分子に限定されず多様であることが判明した。今後生物種間のゲノム情報の比較により分子実体の解明に迫ることで、我々ヒトを含む脊椎動物の体制における電気化学連関の分子機構の原理と多様性の深い理解に繋がる。また、細胞機能の電気的制御などを通じてティシュエンジアリングなど生物工学的応用に繋がる可能性がある。
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Report
(5 results)
Research Products
(91 results)
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[Presentation] Function of voltage-sensing phosphatase in mouse sperm2019
Author(s)
Kawai T,Miyata H, Nakanishi H, Sakata S, Okochi Y, Watanabe M, Fujimoto T, Sakimura K, Sasaki T, Ikawa M, Okamura Y
Organizer
60th International Conference on the Bioscience of Lipids(ICBL2019)
Related Report
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[Presentation] A new mechanism of Ca2+-independent voltage-dependent secretion in dorsal root ganglion neurons2019
Author(s)
Zhuan Zhou, Yuan Wang, Hiroki Arima, Rong Huang, Yuqi Hang, Xingyu Du, Feipeng Zhu, Zuying Chai, Changhe Wang, Yasushi Okamura
Organizer
The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
Related Report
Int'l Joint Research
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[Presentation] Functional analysis of voltage-sensing phosphatase in mouse sperm2019
Author(s)
Takafumi Kawai, Haruhiko Miyata, Hiroki Nakanishi, Souhei Sakata, Yoshifumi Okochi, Masahiko Watanabe, Kenji Sakimura, Takehiko Sasaki, Masahito Ikawa, Yasushi Okamura
Organizer
The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
Related Report
Int'l Joint Research
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[Book] ガイトン生理学 原著第13版2018
Author(s)
石川義弘、岡村康司(総監訳)、尾仲達史、河野憲二
Total Pages
1100
Publisher
エルゼビア・ジャパン株式会社
ISBN
9784860347741
Related Report