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vocal fold regeneration using stacked cell sheets

Research Project

Project/Area Number 18K19609
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
Research InstitutionKyoto University

Principal Investigator

Omori Koichi  京都大学, 医学研究科, 教授 (10233272)

Co-Investigator(Kenkyū-buntansha) 山下 勝  鹿児島大学, 医歯学域医学系, 教授 (10635519)
楯谷 一郎  藤田医科大学, 医学部, 教授 (20526363)
中村 亮介  京都大学, 医学研究科, 研究員 (40736708)
岸本 曜  京都大学, 医学研究科, 助教 (80700517)
青山 朋樹  京都大学, 医学研究科, 教授 (90378886)
Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords声帯 / 上皮細胞 / 線維芽細胞 / 積層化技術 / 積層化 / 繊維芽細胞 / 層構造 / 培養 / バイオ3Dプリンター
Outline of Final Research Achievements

Epithelial cells and fibroblasts were successfully isolated and cultured from rat vocal folds, canine vocal folds and canine oral mucosa. Further, cultured cell sheets were stacked using gelatin hydrogel microspheres. Stacked cell sheets were about 300 μm in thickness, and layered structure in cell sheets were confirmed by immunostaining. Further, preliminary experiments revealed that the cell sheets can be implantated into the canine vocal fold.

Academic Significance and Societal Importance of the Research Achievements

今後はこの積層化細胞シートをイヌの声帯に自家移植し、声帯粘膜の再生効果を組織学的に検討するとともに、吹鳴実験を行いその物性および機能性を評価する予定である。この技術を確立され、細胞シート移植の粘膜再生効果が確認されれば、声帯瘢痕や萎縮などの組織学的な変化を伴う難治性声帯疾患に対する、声帯再生医療の実現に大きく貢献すると考えられる。更に、その技術は他臓器の再生医療にも応用できる可能性がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Alterations in macrophage polarization in injured murine vocal folds.2019

    • Author(s)
      Kaba S, Nakamura R, Yamashita M, Katsuno T, Suzuki R, Tateya I, Kishimoto Y, Omori K.
    • Journal Title

      Laryngoscope.

      Volume: 129(4) Issue: 4

    • DOI

      10.1002/lary.27523

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of aged rat vocal fold fibroblasts2018

    • Author(s)
      Kawai Yoshitaka、Kishimoto Yo、Sogami Tohru、Suzuki Ryo、Tsuji Takuya、Hiwatashi Nao、Tateya Ichiro、Kanemaru Shin-Ichi、Nakamura Tatsuo、Omori Koichi、Hirano Shigeru
    • Journal Title

      The Laryngoscope

      Volume: 129 Issue: 3

    • DOI

      10.1002/lary.27464

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Development of an in vitro model of rat vocal fold epithelium2019

    • Author(s)
      Keisuke Kojima
    • Organizer
      2019 Combined Otolaryngology Spring Meetings
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ラット声帯上皮のin vitroモデルの作成2019

    • Author(s)
      児嶋圭介、吉松誠芳、樋渡直、岸本曜、末廣篤、楯谷一郎、大森孝一
    • Organizer
      第31回日本喉頭科学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Development of an in vitro model of rat vocal fold epithelium2019

    • Author(s)
      Keisuke Kojima, Tatsuya Katsuno, Masanobu Mizuta, Ryosuke Nakamura, Yo Kishimoto, Masayoshi Yoshimatsu, Hiroe Ohnishi, Atsushi Suehiro, Tomoko Tateya, Ichiro Tateya, Koichi Omori
    • Organizer
      140th Annual Meeting of American Laryngological Association
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-07-25   Modified: 2022-01-27  

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