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When did toxic microalgae and drug resistant bacteria appear in Chilean coastal waters and how they spread the distributions?

Research Project

Project/Area Number 18KK0182
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Research InstitutionFisheries Research and Education Agency

Principal Investigator

Nagai Satoshi  国立研究開発法人水産研究・教育機構, 水産技術研究所(横浜), 主幹研究員 (80371962)

Co-Investigator(Kenkyū-buntansha) 安池 元重  国立研究開発法人水産研究・教育機構, 水産資源研究所(横浜), グループ長 (20604820)
本郷 悠貴  国立研究開発法人水産研究・教育機構, 水産資源研究所(横浜), 研究員 (20737316)
宮下 英明  京都大学, 人間・環境学研究科, 教授 (50323746)
及川 寛  国立研究開発法人水産研究・教育機構, 水産資源研究所(横浜), グループ長 (80371855)
安田 仁奈  宮崎大学, 農学部, 准教授 (00617251)
Project Period (FY) 2018-10-09 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2020: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2019: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords分布拡大 / Alexandrium catenella / 集団遺伝学 / 有毒渦鞭毛藻 / 遺伝的多様性 / サケ養殖 / チリ / ショットガンシーケンス / 薬剤耐性菌 / 麻痺性貝毒
Outline of Final Research Achievements

In this study, MIG ISSR (multiplexed Genotyping inter simple sequence repeats )-seq was employed to reveal the genetic relatedness among Pacific Rim populations. We obtained 349 SNPs in 165 samples isolated from Atsukeshi, Sendai, Osaka, Hiroshima Bays, Jinhae Bay (Korea), Bering Sea, Chukchi Sea, Puget Sound (USA) and Chile. Pairwise FST values showed significant differences among most of the pairwise samples except between Osaka & Jinhae Bay and Osaka & Hiroshima Bay. Also, results of the bar plot by STRUCTURE analysis suggested that when K = 3, each cluster was grouped as 1) Japan and Korean except for Akkeshi Bay, 2) Akkeshi Bay and the Bering/Chukchi Sea, 3) Puget Sound and Chilean samples. These data suggested the presence of large genetic breaks between the Bering Sea and Puget Sound populations and genetic connectivity between Akkeshi Bay and the Bering/Chukchi Sea populations.

Academic Significance and Societal Importance of the Research Achievements

日本、韓国、北極海、北米西岸、チリ沿岸から単離した有毒渦鞭毛藻Alexandrium catenellaの集団を対象に、ゲノムワイドな解析手法であるMIG-seqを用いた集団遺伝学解析を実施し、大きく分けて、日本・韓国・北極海の集団で一つのグループ、北米西岸・チリでもう一つのグループに区分され、これら2つのグループの間に大きな遺伝障壁の有ることが示唆された。北海道の厚岸の集団が、北極海の集団と遺伝的に近く、日本国内の集団の起源が北方起源である可能性が示されるなど、新たな知見を得ることができた。世界的に大きな関心のあるA. catenellaの集団分化の歴史の一端が明らかに出来た意義は大きい。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (36 results)

All 2022 2021 2020 Other

All Int'l Joint Research (16 results) Journal Article (8 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 8 results,  Open Access: 7 results) Presentation (10 results) (of which Int'l Joint Research: 10 results,  Invited: 2 results) Book (2 results)

  • [Int'l Joint Research] Instituto de Fomento Pesquero (IFOP)/Antofagasta University/La Frontera University(チリ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of Technology Sydney(オーストラリア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Third Institute of Oceanography(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Alfred Wegener Institut-Helmholtz(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] NOAA(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Instituto de Fomento Pesquero (IFOP)/Universidad de La Frontera/Antofagasta University(チリ)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Bulgarian Academy of Science(ブルガリア)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] National Research Council CNR-IRSA(イタリア)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Tallinn University of Technology(エストニア)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Instituto de Fomento Pesquero/Antofagasta University(チリ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] National Autonomous Univ of Mexico(メキシコ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Pukyong National University(韓国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] NOAA(米国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Instituto de Fomento Pesquero/Antofagasta University(チリ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] National Autonomous Univ of Mexico(メキシコ)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Seasonal genotype dynamics of a marine dinoflagellate: Pelagic populations are homogeneous and as diverse as benthic seed banks2021

    • Author(s)
      Jerney Jacqueline、Rengefors Karin、Nagai Satoshi、Krock Bernd、Sjqvist Conny、Suikkanen Sanna、Kremp Anke
    • Journal Title

      Molecular Ecology

      Volume: 31 Issue: 2 Pages: 512-528

    • DOI

      10.1111/mec.16257

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis2021

    • Author(s)
      Yarimizu Kyoko、Fujiyoshi So、Kawai Mikihiko、Acu?a Jacquelinne J.、Rilling Joaquin-Ignacio、Campos Marco、Vilugr?n Jonnathan、Cameron Henry、Vergara Karen、Gajardo Gonzalo、Espinoza-Gonz?lez Oscar、Guzm?n Leonardo、Nagai Satoshi、Riquelme Carlos、Jorquera Milko A.、Maruyama Fumito
    • Journal Title

      Journal of Visualized Experiments

      Volume: 174 Issue: 174 Pages: 2-16

    • DOI

      10.3791/62967

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plankton biodiversity and species co-occurrence based on environmental DNA ? a multiple marker study2021

    • Author(s)
      Sildever Sirje、Laas Peeter、Kolesova Natalja、Lips Inga、Lips Urmas、Nagai Satoshi
    • Journal Title

      Metabarcoding and Metagenomics

      Volume: 5 Pages: 175-197

    • DOI

      10.3897/mbmg.5.72371

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of an absolute quantification method for ribosomal RNA gene copy numbers per eukaryotic single cell by digital PCR2021

    • Author(s)
      Kyoko Yarimizu, Sirje Sildever, Yoko Hamamoto, Satoshi Tazawa, Hiroshi Oikawa, Haruo Yamaguchi, Leila Basti, Jorge I. Mardones, Javier Paredes-Mella, Satoshi Nagai
    • Journal Title

      Harmful Algae

      Volume: 103 Pages: 102008-102008

    • DOI

      10.1016/j.hal.2021.102008

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Protocols for Monitoring Harmful Algal Blooms for Sustainable Aquaculture and Coastal Fisheries in Chile2020

    • Author(s)
      Yarimizu Kyoko、Fujiyoshi So、Kawai Mikihiko, et al
    • Journal Title

      International Journal of Environmental Research and Public Health

      Volume: 17 Issue: 20 Pages: 7642-7642

    • DOI

      10.3390/ijerph17207642

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Morphological and phylogenetic data do not support the split of Alexandrium into four genera2020

    • Author(s)
      Kenneth Neil Mertens, Masao Adachi, Donald M. Anderson, Satoshi Nagai, et al.
    • Journal Title

      Harmful Algae

      Volume: 98 Pages: 101902-101902

    • DOI

      10.1016/j.hal.2020.101902

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative analysis of morphological and molecular approaches integrated into the study of the dinoflagellate biodiversity within the recently deposited Black Sea sediments - benefits and drawbacks2020

    • Author(s)
      Nina Dzhembekova, Fernando Rubino, Satoshi Nagai, Nataliya Slabakova, Petya Ivanova, Ivelina Zlateva, Violeta Slabakova, Snejana Moncheva
    • Journal Title

      Biodiversity Data Journal

      Volume: 8

    • DOI

      10.3897/bdj.8.e55172

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Suitcase Lab: new, portable, and deployable equipment for rapid detection of specific harmful algae in Chilean coastal waters2020

    • Author(s)
      Fujiyoshi So、Yarimizu Kyoko、Miyashita Yohei、Rilling Joaquin、Acuna Jacquelinne J.、Ueki Shoko、Gajardo Gonzalo、Espinoza-Gonzalez Oscar、Guzm?n Leonardo、Jorquera Milko A.、Nagai Satoshi、Maruyama Fumito
    • Journal Title

      Environmental Science and Pollution Research

      Volume: 28 Issue: 11 Pages: 14144-14155

    • DOI

      10.1007/s11356-020-11567-5

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] First culture of Dinophysis acuminata from southern Chile: Ecophysiology, toxin production and phylogeny2021

    • Author(s)
      Javier Paredes-Mella, Jorge I. Mardones, Luis Norambuena, Gonzalo Fuenzalida and Satoshi Nagai
    • Organizer
      19th International Conference on Harmful Algae
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ichthyotoxicity of mixed blooms of Dinophysis, Alexandrium and Cochlodinium2021

    • Author(s)
      Leila Basti, Jiyoji Go, Satoshi Nagai, Kyohito Nagai
    • Organizer
      19th International Conference on Harmful Algae
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mapping the development of Dinophysis spp. HABs using a novel molecular qPCR assay2021

    • Author(s)
      Hernan F. Henriquez-Nunez, Penelope A. Ajani, Arjun Verma, Satoshi Nagai, Matthew J. Tesoriero, Hazel Farrell, Anthony Zammit, Steve Brett and Shauna A. Murray
    • Organizer
      19th International Conference on Harmful Algae
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Monitoring harmful algal bloom species and their appearance in the Tokyo Bay, Japan, based on metabarcoding and high-throughput sequencing2021

    • Author(s)
      Sirje Sildever, Noriko Nishi, Nobuharu Inaba, Taiga Asakura, Jun Kikuchi, Yasuhito Asano, Satoshi Nagai
    • Organizer
      19th International Conference on Harmful Algae
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of an absolute quantification method for ribosomal RNA gene copy numbers per eukaryotic single cell by digital PCR2021

    • Author(s)
      Satoshi Nagai
    • Organizer
      1st International Congress of Biotechnology and Bioengineering
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 有毒渦鞭毛藻 Dinophysis 属の盗葉緑体・増殖生理生態について2021

    • Author(s)
      長井 敏
    • Organizer
      日本共生生物学会
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Chasing a moving target: The intriguing diversity of goniodomins2021

    • Author(s)
      Bernd Krock, Urban Tillmann, Luisa Hintze, Kristof Moller, Haifeng Gu, Satoshi Nagai, Kim Reece, Aaron J.C. Andersen, Tom Harris
    • Organizer
      19th International Conference on Harmful Algae
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic analysis revealed large genetic breaks among Pacific Rim populations in Alexandrium catenella2021

    • Author(s)
      Satoshi Nagai, Mizuki Horoiwa, Nina Yasuda, Satoshi Tazawa, Sirje Sildever, Masafumi Natsuike, Keigo Yamamoto, Hiroshi Yamauchi, Chang-Hoon Kim, Vera Trainer, Daniel Varela, Jorge I. Mardones, Javier Paredes-Mella, Ichiro Imai
    • Organizer
      19th International Conference on Harmful Algae
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Harmful Algae Monitoring on San Jorge Bay in Antofagasta, Chile2021

    • Author(s)
      Kyoko Yarimizu, Carlos Riquelme, Henry Cameron, Vladimir Avalos, Stephanie Barria, So Fujiyoshi, Joaquin-Ignacio Rilling, Emma-Karin Cascales, Jonnathan Vilugron, Oscar Espinoza-Gonzalez, Leonardo Guzman, Milko A. Jorquera, 長井 敏, Fumito Maruyama
    • Organizer
      19th International Conference on Harmful Algae
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Long-term composition of 16S-based bacterial communities associated with algal bloom events in northern Chile2021

    • Author(s)
      Andres Avila, Mariela Gonzalez-Flores, Milko Jorquera,Joaqura, Joaquin Rilling, Carlos Riquelme, Henry Cameron, Leonardo Guzman, Gonzalo Gajardo, Fumito Maruyama, 長井 敏, Shoko Ueki
    • Organizer
      19th International Conference on Harmful Algae
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Book] Advances in Phytoplankton Ecology2022

    • Author(s)
      Mardones JI, Krock B, MArcus L, Alves-de-Souza C, Nagai S, Yarimizu K, Clement A, Correa N, Silva S, Paredes-Mella J, Dassow PV
    • Total Pages
      36
    • Publisher
      Elsevier
    • Related Report
      2021 Annual Research Report
  • [Book] Dinoflagellates - Classification, evolution, physiology and ecological significance2020

    • Author(s)
      Satoshi Nagai, Sirje Sildever, Toshiyuki Suzuki, Goh Nishitani, Leila Basti, Takashi Kamiyama
    • Total Pages
      729
    • Publisher
      Nova Scientific Publishers, Inc.
    • ISBN
      9781536178883
    • Related Report
      2020 Research-status Report

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Published: 2018-10-12   Modified: 2023-01-30  

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