Field-based toxicological mechanism analysis of emerging and re-emerging environmental pollution in Africa.

• Project/Area Number: 18KK0287
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 63:Environmental analyses and evaluation and related fields
• Research Institution: Hokkaido University
• Principal Investigator: Ishizuka Mayumi 北海道大学, 獣医学研究院, 教授 (50332474)
• Co-Investigator(Kenkyū-buntansha): 池中 良徳 北海道大学, 獣医学研究院, 教授 (40543509) ; 中山 翔太 北海道大学, 獣医学研究院, 准教授 (90647629) ; 中田 北斗 北海道大学, 獣医学研究院, 博士研究員 (60815273)
• Project Period (FY): 2018-10-09 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2021: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: アフリカ / 環境汚染 / 農薬 / 重金属 / 環境毒性 / 毒性 / ザンビア / 南アフリカ / ガーナ

Outline of Final Research Achievements

In recent years, African countries have been promoting rapid resource development. As a result, rapid pollution of the environment has become apparent, and health hazards to ecosystems, livestock, and humans have been reported in some countries. For example, most of the 230,000 lead poisoning deaths worldwide occur in developing countries such as Africa, and the WHO has indicated that lead should be a priority. In this study, we analyzed exposure to heavy metals and pesticides using Zambia and South Africa as models to determine the actual effects on humans and animals that are occurring at the field level.

Academic Significance and Societal Importance of the Research Achievements

アフリカにおける環境汚染はブラックボックスとなっているが、本研究ではヒトや動物が直接受けている環境汚染とその生体影響について明らかにした。実際に症状が顕在化する前にどのような変化が起こりえるのかを遺伝子レベルやメタボローム解析により報告した。重金属ではひとで起こっているゲノムの修飾の変化を明らかにし、また、農薬では胆汁酸に係る変化を新たに報告した。

Research Products

• [Int'l Joint Research] ザンビア大学(ザンビア)
• [Int'l Joint Research] ノースウェスト大学(南アフリカ)
• [Int'l Joint Research] クワメエンクルマ科学技術大学(ガーナ)
• [Int'l Joint Research] ザンビア大学(ザンビア)
• [Int'l Joint Research] ノースウェスト大学(南アフリカ)
• [Int'l Joint Research] クワメエンクルマ科学技術大学(ガーナ)
• [Int'l Joint Research] ザンビア大学(ザンビア)
• [Int'l Joint Research] ノースウェスト大学(南アフリカ)
• [Int'l Joint Research] クワメエンクルマ科学技術大学(ガーナ)
• [Int'l Joint Research] ザンビア大学(ザンビア)
• [Int'l Joint Research] クワメエンクルマ科学技術大学(ガーナ)
• [Int'l Joint Research] ノースウェスト大学(南アフリカ)
• [Int'l Joint Research] クワメエンクルマ科学技術大学(ガーナ)
• [Int'l Joint Research] ザンビア大学(ザンビア)
• [Int'l Joint Research] ノースウェスト大学(南アフリカ)
• [Journal Article] Environmentally relevant lead (Pb) water concentration induce toxicity in zebrafish (Danio rerio) larvae (2022)
  • Author(s): Kataba Andrew、Botha Tarryn L.、Nakayama Shouta M.M.、Yohannes Yared B.、Ikenaka Yoshinori、Wepener Victor、Ishizuka Mayumi
  • Journal Title: Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology Volume: 252 Pages: 109215-109215
  • DOI: 10.1016/j.cbpc.2021.109215
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Methylation profiles of global LINE-1 DNA and the GSTP1 promoter region in children exposed to lead (Pb) (2022)
  • Author(s): Yohannes Yared Beyene、Nakayama Shouta M.M.、Yabe John、Toyomaki Haruya、Kataba Andrew、Nakata Hokuto、Muzandu Kaampwe、Miyashita Chihiro、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Epigenetics Volume: 17 Issue: 13 Pages: 2377-2388
  • DOI: 10.1080/15592294.2022.2123924
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study (2022)
  • Author(s): Nakata Hokuto、Eguchi Akifumi、Nakayama Shouta M. M.、Yabe John、Muzandu Kaampwe、Ikenaka Yoshinori、Mori Chisato、Ishizuka Mayumi
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 19 Issue: 1 Pages: 541-541
  • DOI: 10.3390/ijerph19010541
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Glutathione S-transferase gene polymorphisms in association with susceptibility to lead toxicity in lead- and cadmium-exposed children near an abandoned lead-zinc mining area in Kabwe, Zambia (2021)
  • Author(s): Yohannes Yared Beyene、Nakayama Shouta M. M.、Yabe John、Toyomaki Haruya、Kataba Andrew、Nakata Hokuto、Muzandu Kaampwe、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Environmental Science and Pollution Research Volume: 29 Issue: 5 Pages: 6622-6632
  • DOI: 10.1007/s11356-021-16098-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Human Exposures to Neonicotinoids in Kumasi, Ghana (2021)
  • Author(s): Collins Nimako,Yoshinori Ikenaka,Osei Akoto,Nesta Bortey-Sam,Takahiro Ichise,Shouta M.M. Nakayama,Kwadwo A. Asante,Kazutoshi Fujioka,Kumiko Taira,Mayumi Ishizuka
  • Journal Title: Environmental Toxicology and Chemistry Volume: 40 Issue: 8 Pages: 2306-2318
  • DOI: 10.1002/etc.5065
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The impact of elevated blood lead levels in children on maternal health-related quality of life (2021)
  • Author(s): Nakata Hokuto、Tohyama Harukazu、Fujita Wakako、Nakayama Shouta M.M.、Ishizuka Mayumi、Yabe John、Munyinda Nosiku S.、Sakala Doreen、Choongo Kennedy、Yamasaki Shojiro、Nagai Natsumi、Yoshida Takahiko、Saito Takeshi
  • Journal Title: Chemosphere Volume: - Pages: 130490-130490
  • DOI: 10.1016/j.chemosphere.2021.130490
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Assessment of LeadCare? II analysis for testing of a wide range of blood lead levels in comparison with ICP?MS analysis (2021)
  • Author(s): Nakata Hokuto、Nakayama Shouta M.M.、Yabe John、Muzandu Kaampwe、Toyomaki Haruya、Yohannes Yared Beyene、Kataba Andrew、Zyambo Golden、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Chemosphere Volume: 271 Pages: 129832-129832
  • DOI: 10.1016/j.chemosphere.2021.129832
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An Investigation of the Wild Rat Crown Incisor as an Indicator of Lead (Pb) Exposure Using Inductively Couple Plasma Mass Spectrometry (ICP-MS) and Laser Ablation ICP-MS (2021)
  • Author(s): Kataba Andrew、Nakayama Shouta M. M.、Nakata Hokuto、Toyomaki Haruya、Yohannes Yared B.、Yabe John、Muzandu Kaampwe、Zyambo Golden、Kubota Ayano、Matsukawa Takehisa、Yokoyama Kazuhito、Ikenaka Yoshinori、Ishizuka Mayumi
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 18 Issue: 2 Pages: 767-767
  • DOI: 10.3390/ijerph18020767
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of the ameliorative effect of Spirulina (Arthrospira platensis) supplementation on parameters relating to lead poisoning and obesity in C57BL/6J mice (2021)
  • Author(s): Nakata Hokuto、Nakayama Shouta M.M.、Kataba Andrew、Yohannes Yared Beyene、Ikenaka Yoshinori、Ishizuka Mayumi
  • Journal Title: Journal of Functional Foods Volume: 77 Pages: 104344-104344
  • DOI: 10.1016/j.jff.2020.104344
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Delta-aminolevulinic acid dehydratase (ALAD) and vitamin D receptor (VDR) genes polymorphisms in children residing in an abandoned lead?zinc mine area in Kabwe, Zambia (2021)
  • Author(s): Yohannes Yared B.、Nakayama Shouta M.M.、Yabe John、Toyomaki Haruya、Kataba Andrew、Nakata Hokuto、Muzandu Kaampwe、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Meta Gene Volume: 27 Pages: 100838-100838
  • DOI: 10.1016/j.mgene.2020.100838
  • NAID: 120007187740
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Levels of DDTs and other organochlorine pesticides in healthy wild Nile crocodiles (Crocodylus niloticus) from a flagship conservation area (2021)
  • Author(s): Gerber Ruan、Bouwman Hindrik、Govender Danny、Ishizuka Mayumi、Ikenaka Yoshinori、Yohannes Yared B.、Smit Nico J.、Wepener Victor
  • Journal Title: Chemosphere Volume: 264 Pages: 128368-128368
  • DOI: 10.1016/j.chemosphere.2020.128368
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Clinical biochemical parameters associated with the exposure to multiple environmental metals in residents from Kabwe, Zambia (2021)
  • Author(s): Nakata Hokuto、Nakayama Shouta M.M.、Yabe John、Muzandu Kaampwe、Toyomaki Haruya、Yohannes Yared Beyene、Kataba Andrew、Zyambo Golden、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Chemosphere Volume: 262 Pages: 127788-127788
  • DOI: 10.1016/j.chemosphere.2020.127788
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Acute exposure to environmentally relevant lead levels induces oxidative stress and neurobehavioral alterations in larval zebrafish (Danio rerio) (2020)
  • Author(s): Kataba Andrew、Botha Tarryn L.、Nakayama Shouta M.M.、Yohannes Yared B.、Ikenaka Yoshinori、Wepener Victor、Ishizuka Mayumi
  • Journal Title: Aquatic Toxicology Volume: 227 Pages: 105607-105607
  • DOI: 10.1016/j.aquatox.2020.105607
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Land Use in Habitats Affects Metal Concentrations in Wild Lizards Around a Former Lead Mining Site (2020)
  • Author(s): Doya Rio、Nakayama Shouta M. M.、Nakata Hokuto、Toyomaki Haruya、Yabe John、Muzandu Kaampwe、Yohannes Yared B.、Kataba Andrew、Zyambo Golden、Ogawa Takahiro、Uchida Yoshitaka、Ikenaka Yoshinori、Ishizuka Mayumi
  • Journal Title: Environmental Science & Technology Volume: 54 Issue: 22 Pages: 14474-14481
  • DOI: 10.1021/acs.est.0c00150
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Blood lead levels and aberrant DNA methylation of the ALAD and p16 gene promoters in children exposed to environmental-lead (2020)
  • Author(s): Yohannes Yared B.、Nakayama Shouta MM.、Yabe John、Nakata Hokuto、Toyomaki Haruya、Kataba Andrew、Muzandu Kaampwe、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Environmental Research Volume: 188 Pages: 109759-109759
  • DOI: 10.1016/j.envres.2020.109759
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Factors associated with lead (Pb) exposure on dogs around a Pb mining area, Kabwe, Zambia (2020)
  • Author(s): Toyomaki Haruya、Yabe John、Nakayama Shouta M.M.、Yohannes Yared B.、Muzandu Kaampwe、Liazambi Allan、Ikenaka Yoshinori、Kuritani Takeshi、Nakagawa Mitsuhiro、Ishizuka Mayumi
  • Journal Title: Chemosphere Volume: 247 Pages: 125884-125884
  • DOI: 10.1016/j.chemosphere.2020.125884
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Current trends of blood lead levels, distribution patterns and exposure variations among household members in Kabwe, Zambia (2020)
  • Author(s): Yabe John、Nakayama Shouta MM.、Nakata Hokuto、Toyomaki Haruya、Yohannes Yared B.、Muzandu Kaampwe、Kataba Andrew、Zyambo Golden、Hiwatari Masato、Narita Daiju、Yamada Daichi、Hangoma Peter、Munyinda Nosiku Sipilanyambe、Mufune Tiza、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Chemosphere Volume: 243 Pages: 125412-125412
  • DOI: 10.1016/j.chemosphere.2019.125412
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bioimaging of Pb and STIM1 in mice liver, kidney and brain using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) and immunohistochemistry (2020)
  • Author(s): Togao Masao、Nakayama Shouta M.M.、Ikenaka Yoshinori、Mizukawa Hazuki、Makino Yoshiki、Kubota Ayano、Matsukawa Takehisa、Yokoyama Kazuhito、Hirata Takafumi、Ishizuka Mayumi
  • Journal Title: Chemosphere Volume: 238 Pages: 124581-124581
  • DOI: 10.1016/j.chemosphere.2019.124581
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dichlorodiphenyltrichloroethane (DDT) levels in rat livers collected from a malaria vector control region (2019)
  • Author(s): MOTOHIRA Kodai、IKENAKA Yoshinori、YOHANNES Yared Beyene、NAKAYAMA Shouta M. M.、WEPENER Victor、SMIT Nico J.、VAN VUREN Johan H. J.、SOUSA Ana Catarina、ENUNEKU Alex Ajeh、OGBOMIDA Emmanuel Temiotan、ISHIZUKA Mayumi
  • Journal Title: Journal of Veterinary Medical Science Volume: 81 Issue: 11 Pages: 1575-1579
  • DOI: 10.1292/jvms.19-0168
  • NAID: 130007744347
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] How toxic is a non-toxic nanomaterial: Behaviour as an indicator of effect in Danio rerio exposed to nanogold (2019)
  • Author(s): Botha Tarryn L.、Brand Sarel J.、Ikenaka Yoshinori、Nakayama Shouta M.M.、Ishizuka Mayumi、Wepener Victor
  • Journal Title: Aquatic Toxicology Volume: 215 Pages: 105287-105287
  • DOI: 10.1016/j.aquatox.2019.105287
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of the organochlorine p,p’-DDT on MCF-7 cells: Investigating metabolic and immune modulatory transcriptomic changes (2019)
  • Author(s): Thompson Lesa A.、Ikenaka Yoshinori、Sobhy Darwish Wageh、Nakayama Shouta M.M.、Mizukawa Hazuki、Ishizuka Mayumi
  • Journal Title: Environmental Toxicology and Pharmacology Volume: 72 Pages: 103249-103249
  • DOI: 10.1016/j.etap.2019.103249
  • NAID: 120006941695
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] One year exposure to Cd- and Pb-contaminated soil causes metal accumulation and alteration of global DNA methylation in rats (2019)
  • Author(s): Nakayama Shouta M.M.、Nakata Hokuto、Ikenaka Yoshinori、Yabe John、Oroszlany Balazs、Yohannes Yared B.、Bortey-Sam Nesta、Muzandu Kaampwe、Choongo Kennedy、Kuritani Takeshi、Nakagawa Mitsuhiro、Ishizuka Mayumi
  • Journal Title: Environmental Pollution Volume: 252 Pages: 1267-1276
  • DOI: 10.1016/j.envpol.2019.05.038
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dietary responses of the spotted killifish Nothobranchius orthonotus to drought in an African subtropical temporary pool using stable δ13C and δ15N isotopic analysis. (2019)
  • Author(s): De Necker L, Manfrin A, Ikenaka Y, Ishizuka M, Brendonck L, van Vuren J.H.J, Wepener V, Smit N.J.
  • Journal Title: Journal of Fish Biology (in press) Volume: -
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] aseline bio-accumulation concentrations and resulting oxidative stress in Synodontis zambezensis after an acute laboratory exposure to 4,4’-DDT. Pesticide Biochemistry and Physiology. (2019)
  • Author(s): C.M. Volschek, Y. Ikenaka, Y.B. Yohannes, S.M. Nakayama, M. Ishizuka, E. Smit, J.H.J. van Vuren, R. Greenfield.
  • Journal Title: Pesticide Biochemistry and Physiology (in press) Volume: - Pages: 44-55
  • DOI: 10.1016/j.pestbp.2019.02.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evidence of impacts from DDT in pelican, cormorant, stork, and egret eggs from KwaZulu-Natal, South Africa. (2019)
  • Author(s): H. Bouwman, Y.B. Yohannes, S.M.M. Nakayama, K. Motohira, M. Ishizuka, M.S. Humphries, V. van der Schyff, M. du Preez, A. Dinkelmann, Y. Ikenaka.
  • Journal Title: Chemosphere (in press) Volume: - Pages: 647-658
  • DOI: 10.1016/j.chemosphere.2019.03.043
  • Peer Reviewed / Int'l Joint Research
• [Presentation] ザンビアにおける鉛汚染とヒト・動物への健康影響 (2022)
  • Author(s): 中山翔太、石塚真由美
  • Organizer: 日本薬学会第143年会
  • Invited
• [Presentation] Global and Gene-Specific Methylation in Lead-Exposed Children in Kabwe, Zambia (2022)
  • Author(s): Yared Beyene Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: SOT 62nd Annual Meeting and ToxExpo
  • Int'l Joint Research
• [Presentation] Impact of Lead on Human Health: a Kabwe, Zambia perspective (2022)
  • Author(s): Yabe J, Nakayama S, Yohannes YB, Nakata H, Toyomaki H, Muzandu K, Kataba A, Zyambo G, Moonga G, Munyinda NS, Mufune T, Liazambi A, Chawinga K, Sakala D, Ikenaka Y, Choongo K, Ishizuka M
  • Organizer: BirdLife South Africa Lead Advocacy Workshop for the Conservation of Southern African Vultures.
  • Int'l Joint Research
• [Presentation] Distribution of lead in organs of stray dogs from Kabwe, Zambia (2022)
  • Author(s): Nyein Chan Soe, Andrew Kataba, Yared B. Yohannes, Madalitso Tembo, John Yabe, Kenneth Chawinga, Golden Zyambo, Kaampwe Muzandu, Yoshinori Ikenaka1, Mayumi Ishizuka, Shouta M.M. Nakayama
  • Organizer: 6th International Chemical Hazard Symposium ＆ 6th Symposium of Japan Society for Environmental Chemistry
  • Int'l Joint Research
• [Presentation] Investigating Mechanisms of High Metal Accumulation in Wild Lizards in Zambia by XAFS Analysis of Lead and Sulfur Chemical Species (2022)
  • Author(s): Rio Doya, Shouta M.M. Nakayama, Takashi Fujimori, Kenji Shiota, Hokuto Nakata, Golden Zyambo, Nyein Chan Soe, Andrew Kataba, Kaampwe Muzandu, John Yabe, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 6th International Chemical Hazard Symposium ＆ 6th Symposium of Japan Society for Environmental Chemistry
  • Int'l Joint Research
• [Presentation] Environmental, animal and human health impacts of lead and other heavy metals arising from unsustainable mining activities in Kabwe, Zambia: a One Health Approach (2022)
  • Author(s): John Yabe, Shouta MM Nakayama, Yared B Yohannes, Hokuto Nakata, Haruya Toyomaki, Kaampwe Muzandu, Andrew Kataba, Golden Zyambo, Nosiku Sipilanyambe Munyinda, Tiza Mufune, Allan Liazambi, Kenneth Chawinga, Yoshinori Ikenaka, Kennedy Choongo, Mayumi Ishizuka
  • Organizer: 6th International Chemical Hazard Symposium ＆ 6th Symposium of Japan Society for Environmental Chemistry
  • Int'l Joint Research / Invited
• [Presentation] Metal accumulation and toxicity evaluation in terrestrial environment using wild rodents and lizards. (2022)
  • Author(s): Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Rio Doya, Kaampwe Muzandu, Akifumi Eguchi, Yared Yohannes, Yoshinori Ikenaka, Golden Zyambo, Andrew Kataba, Mayumi Ishizuka.
  • Organizer: SETAC North America 43rd Annual Meeting.
  • Int'l Joint Research
• [Presentation] Do organic dietary interventions offer significant ameliorative benefits for human exposure to emerging pesticides? (Laying the emphasis on neonicotinoids). (2022)
  • Author(s): Collins NIMAKO, Anri HIRAI, Takahiro ICHISE, Shouta M.M. NAKAYAMA, Mayumi ISHIZUKA, Yoshinori IKENAKA
  • Organizer: SETAC North America 43rd Annual Meeting
  • Int'l Joint Research
• [Presentation] Zambiaの鉛高濃度汚染地域と低濃度汚染地域における野生ラットの鉛蓄積量及び糖代謝関連遺伝子発現量の解析 (2022)
  • Author(s): 石原清伍, 中田北斗, John Yabe, Andrew Kataba, Kaampwe Muzandu, Golden Zyambo, 本平航大, 銅谷理緒, Yared Yohannes, 近藤充希, 一瀬貴大, 池中良徳, 石塚真由美, 中山翔太
  • Organizer: 第165回日本獣医学会学術集会
• [Presentation] Field and mechanism-based toxicity research on pesticides in Africa (2022)
  • Author(s): Mayumi Ishizuka.
  • Organizer: “Japan-Africa Science Innovation Week” on the occasion of the TICAD VII
  • Int'l Joint Research / Invited
• [Presentation] Analysis of Lead Accumulation and Expression of Genes related to Glucose Metabolism in Wild Rats in High and Low Lead Contaminated Areas in Zambia. (2022)
  • Author(s): Seigo ISHIHARA, Hokuto Nakata, John Yabe, Andrew Kataba, Kaampwe Muzandu, Golden Zyambo, Kodai Motohira, Yared Beyene Yohannes, Rio Doya, Mitsuki Kondo, Takahiro Ichise, Yoshinori Ikenaka, Mayumi Ishizuka, Shouta Nakayama
  • Organizer: International Forum: Interdisciplinary Challenge Mine and Environment.
  • Int'l Joint Research
• [Presentation] Land use and Dispersion Pattern Affect Exposure Levels of Lizard to Lead in Kabwe. (2022)
  • Author(s): Rio Doya, Shouta M.M. Nakayama, Hokuto Nakata, Golden Zyambo, John Yabe, Kaampwe Muzandu, Yoshinori Ikenaka, and Mayumi Ishizuka
  • Organizer: International Forum: Interdisciplinary Challenge Mine and Environment.
  • Int'l Joint Research
• [Presentation] Genome-wide analysis of DNA methylation in canine brain from Kabwe, Zambia. (2022)
  • Author(s): Nyein Chan Soe, Jumpei Yamazaki, John Yabe, Kaampwe Muzandu, Andrew Kataba, Golden Zyambo, Hokuto Nakata, Kenneth Chawiga, Allan Liazambi, Yared B. Yohannes, Yoshinori Ikenaka, Mayumi Ishizuka, Shouta M.M. Nakayama
  • Organizer: International Forum: Interdisciplinary Challenge Mine and Environment.
  • Int'l Joint Research
• [Presentation] ead Exposure and its Epi-(Genetic) Effects on Children in Kabwe, Zambia (2022)
  • Author(s): YARED Beyene Yohannes, Nakayama S.M.M, Yabe J, Haruya T, Kataba A, Muzandu K, Nakata H, Ikenaka Y, Choongo K, Ishizuka M.
  • Organizer: International Forum: Interdisciplinary Challenge Mine and Environment.
  • Int'l Joint Research
• [Presentation] Lead and Heavy Metals arising from unsustainable Mining activities in Kabwe, Zambia. (2022)
  • Author(s): Yabe J, Nakayama S, Nakata H, Yohannes YB, Toyomaki H, Muzandu K, Moonga G, Kataba A, Zyambo G, Munyinda NS, Mufune T, Liazambi A, Choongo K, Ishizuka M.
  • Organizer: International Forum: Interdisciplinary Challenge Mine and Environment.
  • Int'l Joint Research
• [Presentation] In vivo accumulation of different lead species in mice under environmentally relevance circumstance (2022)
  • Author(s): Nyein Chan Soe, Takamitsu Ohigashi, Hokuto Nakata, Chikae Tatsumi, Yoshitaka Uchida, Walubita Mufalo, Mayumi Ito, Tsutomu Sato, Toshifumi Igarashi, Yared B. Yohannes, Yoshinori Ikenaka, Mayumi Ishizuka, Shouta M.M. Nakayama
  • Organizer: 第49回日本毒性学会学術年会
• [Presentation] Assessing the Net Benefits of Organic Farming interventions in Ecosystem Preservation against Emerging Pesticides (2022)
  • Author(s): Collins NIMAKO, Anri HIRAI, Takahiro ICHISE, Shouta M.M. NAKAYAMA, Mayumi ISHIZUKA, Yoshinori IKENAKA
  • Organizer: 第49回日本毒性学会学術年会
• [Presentation] Wild Lizards as a Target of Heavy Metal Biomonitoring Focusing on Land Use Patterns; Example from a Former Mining Site in Zambia (2022)
  • Author(s): Rio Doya, Shouta M.M. Nakayama, Hokuto Nakata, Mitsuki Kondo, Golden Zyambo, John Yabe, Kaampwe Muzandu, Yoshinori Ikenaka, and Mayumi Ishizuka
  • Organizer: 第49回日本毒性学会学術年会
• [Presentation] 鉛と硫黄の化学形態から野生トカゲの生体内重金属類高濃度要因を探る～ザンビア共和国鉱床地域での研究例～ (2022)
  • Author(s): 銅谷 理緒，中山 翔太，藤森 崇，塩田 憲司，中田 北斗，Golden Zyambo，Nyein Chan Soe，Andrew Kataba，Kaampwe Muzandu，John Yabe，池中 良徳，石塚 真由美
  • Organizer: 環境化学物質3学会合同大会
• [Presentation] アフリカにおける環境汚染の現状 (2022)
  • Author(s): 石塚真由美
  • Organizer: 環境化学物質3学会合同大会
  • Invited
• [Presentation] ead Poisoning Among Residents of the Zambian Settlement of KABWE, Particularly Among Children (2022)
  • Author(s): YARED Beyene Yohannes, Nakayama S.M.M, Yabe J, Haruya T, Kataba A, Muzandu K, Nakata H, Ikenaka Y, Choongo K, Ishizuka M
  • Organizer: Japan Geoscience Union (JpGU) Meeting
  • Invited
• [Presentation] ザンビア・カブエ地区における高濃度鉛汚染を示した犬のゲノムワイドなDNAメチル化解析 (2022)
  • Author(s): 山﨑 淳平、豊巻 治也、中山 翔太、Yabe John、Muzandu Kaampwe、Jelinek Jaroslav、横山 晶子、池中 良徳、滝口 満喜、石塚 真由美
  • Organizer: Japan Geoscience Union (JpGU) Meeting
• [Presentation] Land-use Based Mapping of Lead Exposure Risk around a Former Mining Site in Zambia, using Lizard Biomonitoring Data. (2022)
  • Author(s): 銅谷 理緒、中山 翔太、中田 北斗、Yabe John、Muzandu Kaampwe、池中 良徳、石塚 真由美
  • Organizer: Japan Geoscience Union (JpGU) Meeting
• [Presentation] Assessing the Net Benefits of Organic Farming interventions in Ecosystem Preservation against Emerging Pesticides (2022)
  • Author(s): Collins NIMAKO, Anri HIRAI, Takahiro ICHISE, Shouta M.M. NAKAYAMA, Mayumi ISHIZUKA, Yoshinori IKENAKA
  • Organizer: Japan Geoscience Union (JpGU) Meeting
• [Presentation] Immobilization of lead in soil by chicken manure and its efficacy on a mouse model (2022)
  • Author(s): Nyein Chan Soe, Takamitsu Ohigashi, Hokuto Nakata, Chikae Tatsumi, Yoshitaka Uchida, Walubita Mufalo, Mayumi Ito, Tsutomu Sato, Toshifumi Igarashi, Yared B. Yohannes, Yoshinori Ikenaka, Mayumi Ishizuka, Shouta M.M. Nakayama
  • Organizer: Japan Geoscience Union (JpGU) Meeting
• [Presentation] Investigation of major sources of human exposure and estimation of exposure reduction effects by environmental remediation using IEUBK model in lead-contaminated area (2021)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, John Yabe, Kaampwe Muzandu, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 環境化学討論会
• [Presentation] (Epi) Genetic Effects of δ-Aminolevulinic Acid Dehydratase (ALAD) in Children Exposed to Environmental Lead: a primary study from Kabwe, Zambia (2021)
  • Author(s): Yared Beyene Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka.
  • Organizer: 日本毒性学会
• [Presentation] Investigation of Human Exposure Sources and Estimation of Exposure Reduction Effects by Environmental Remediation Using IEUBK Model in Lead-contaminated Area. S (2021)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, John Yabe, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: SETAC North America
  • Int'l Joint Research
• [Presentation] Simultaneous Quantification of Imidacloprid and its Metabolites in Tissues of Mice upon Chronic Low-dose Administration of Imidacloprid. (2021)
  • Author(s): Collins Nimako, Yoshinori Ikenaka, Osei Akoto, Kazutoshi Fujioka, Kumiko Taira, Koji Arizono, Keisuke Kato, Keisuke Takahashi, Shouta M.M. Nakayama, Takahiro Ichise and Mayumi Ishizuka.
  • Organizer: 日本毒性学会
• [Presentation] Genetic Polymorphism and Promoter Methylation of δ-Aminolevulinic Acid Dehydratase (δ-ALAD) in Children from Kabwe, Zambia. (2021)
  • Author(s): Yared Beyene Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka.
  • Organizer: International Chemical Hazard Symposium.
  • Int'l Joint Research
• [Presentation] Does Spirulina (Arthrospira platensis) Supplementation Ameliorate Lead Poisoning and Obesity in C57BL/6J mice? (2020)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, Andrew Kataba, Yared Beyene Yohannes, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: SETAC North America 41st Annual Meeting
  • Int'l Joint Research
• [Presentation] Environmentally relevant lead levels induce neuromuscular, cardiovascular and oxidative stress systems derangements in zebrafish embryos. (2020)
  • Author(s): Andrew Kataba, Tarryn L. Botha, Shouta M.M Nakayama, Yared B. Yohannes, Yoshinori Ikenaka, Victor Wepener and Mayumi Ishizuka
  • Organizer: SETAC North America 41st Annual Meeting
  • Int'l Joint Research
• [Presentation] OLLABORATIVE WORK WITH MONGOLIA : EASY, CHEAP AND EFFICIENT METHOD DEVELOPMENT FOR DRUG RESIDUE IN ANIMAL PRODUCTS (2020)
  • Author(s): Kodai Motohira, Yoshinori Ikenaka, Delgermurun Dugar, Takahiro Ichise, Shouta Nakayama, Mayumi Ishizuka
  • Organizer: The 8th Sapporo Summer Symposium for One Health
  • Int'l Joint Research
• [Presentation] An adverse outcome pathway-based assessment of steatogenic effects of imidacloprid in livers of C57BL/6J Male Mice. (2020)
  • Author(s): Collins NIMAKO, Yoshinori IKENAKA, Yuko OKAMATSU-OGURA, Jussiaea V. BARIUAN, Atsushi KOBAYASHI, Ryo YAMAZAKI, Shouta M.M. NAKAYAMA, and Mayumi ISHIZUKA
  • Organizer: The 8th Sapporo Summer Symposium for One Health
  • Int'l Joint Research
• [Presentation] Embryonic lead exposure induced development toxicity, neuromuscular, cardiovascular and oxidative stress responses in zebrafish (Danio rerio) (2020)
  • Author(s): Andrew Kataba, Tarryn L. Botha, Shouta M.M Nakayama, Yared B. Yohannes, Yoshinori Ikenaka, Victor Wepener and Mayumi Ishizuka
  • Organizer: The 8th Sapporo Summer Symposium for One Health
  • Int'l Joint Research
• [Presentation] Effects of environmentally and regulatory lead (Pb) levels on survival, neuromuscular, cardiovascular and oxidative stress systems in zebrafish embryos (2020)
  • Author(s): Andrew Kataba, Tarryn L. Botha, Shouta M.M Nakayama, Yared B. Yohannes, Yoshinori Ikenaka, Victor Wepener and Mayumi Ishizuka
  • Organizer: 環境化学オンライン研究発表会2020
• [Presentation] An adverse outcome pathway-based assessment of steatogenic effects of imidacloprid in livers of C57BL/6J Male Mice (2020)
  • Author(s): Collins NIMAKO, Yoshinori IKENAKA, Yuko OKAMATSU-OGURA, Jussiaea V. BARIUAN, Atsushi KOBAYASHI, Ryo YAMAZAKI, Shouta M.M. NAKAYAMA, and Mayumi ISHIZUKA
  • Organizer: 環境化学オンライン研究発表会2020
• [Presentation] アフリカのザンビア共和国カブウェでの地下水の鉛汚染の移動経路の解明 (2020)
  • Author(s): Shota NAKANO, Kazuhiro TOYODA, Shunitz TANAKA, Kawawa BANDA, Imasiku A. NYAMBE, Tsuyoshi ISHIKAWA, Shouta MM NAKAYAMA, Mayumi ISHIZUKA
  • Organizer: 放射化分析及び中性子を用いた地球化学的研究
• [Presentation] Gene Specific Promoter Methylation of Lead Exposure Biomarker Genes in Environmental Lead-Exposed Children from Kabwe, Zambia (2020)
  • Author(s): YARED B. Yohannes, Shouta Nakayama, John Yabe, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Hokuto Nakata, Yoshinori Ikenaka, Kennedy Choongo, Mayumi Ishizuka.
  • Organizer: The 2020 SOT Annual Meeting
  • Int'l Joint Research
• [Presentation] The trend of elevated blood lead levels in Kabwe mining area, Republic of Zambia. (2019)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, John Yabe, Kaampwe Muzandu, Yared B. Yohannes, Haruya Toyomaki, Andrew Kataba, Golden Zyambo, Hazuki Mizukawa, Yoshinori Ikenaka, Russel Dowling, Jack Caravanos, Mayumi Ishizuka.
  • Organizer: SETAC Europe 29th Annual Meeting
  • Int'l Joint Research
• [Presentation] Cost-effective mitigation of lead toxicity in rat using indigenous plant Moringa Oleifera. (2019)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, Andrew Kataba, Haruya Toyomaki, Rio Doya, John Yabe, Kaampwe Muzandu, Golden Zyambo, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka.
  • Organizer: SETAC Europe 29th Annual Meeting
  • Int'l Joint Research
• [Presentation] odent tooth as a tool for environmental lead (Pb) assessment (2019)
  • Author(s): Andrew Kataba, Shouta M.M. Nakayama, Hokuto Nakata,Haruya Toyomaki,Yared B. Yohannes,Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] Neonicotinoid Insecticide and High Fat Diet Interactions Potentiate Sidnals of Type 2 Diabetogenesis in a Rodent Model (2019)
  • Author(s): Collins Nimako, Yoshinori Ikenaka, Yuko Okamatsu-Ogura, Atsushi Kobayashi, Shouta M.M. Nakayama, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] DNA methylation in CpG-rich promoter region and Genetic polymorphism of ALAD gene in environmental Lead-Exposed Children from Kabwe, Zambia (2019)
  • Author(s): Yared B. Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, , Kaampwe Muzandu, Andrew Kataba,Yoshinori Ikenaka, Kennedy Choongo, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] Seasonal change of lead contamination levels in wild rat blood and soil (2019)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, Golden Zyambo, John Yabe, Kaampwe Muzandu, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] ザンビア共和国カブエ地域における資源採掘に起因した鉛汚染の実態解明と対策の検討 (2019)
  • Author(s): 中田北斗、中山翔太、John Yabe、Kaampwe Muzandu、豊巻治也、Yared Beyene、Andrew Kataba、Golden Zyambo、Kennedy Choongo、池中良徳、石塚真由美
  • Organizer: 第28回環境化学討論会
• [Presentation] Investigation of ameliorative effect of Moringa Oleifera on lead toxicity (2019)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, Andrew Kataba, Haruya Toyomaki, Rio Doya, John Yabe, Kaampwe Muzandu, Golden Zyambo, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] Neonicotinoid Insecticide and High Fat Diet Interactions Potentiate Sidnals of Type 2 Diabetogenesis in a Rodent Model (2019)
  • Author(s): Collins Nimako, Yoshinori Ikenaka, Yuko Okamatsu-Ogura, Atsushi Kobayashi, Ryo Yamazaki, Shouta M.M. Nakayama, Mayumi Ishizuka
  • Organizer: SETAC-Australasia
  • Int'l Joint Research
• [Presentation] Determination of Lead (Pb) exposure on infants living around Pb mining area in Kabwe, Zambia (2019)
  • Author(s): Haruya Toyomaki, John Yabe, Shouta Nakayama, Yared Yohannes, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Hokuto Nakata, Takeshi Kuritani, Mitsuhiro Nakagawa, Mayumi Ishizuka
  • Organizer: International Congress of Toxicology
  • Int'l Joint Research
• [Presentation] Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) reveals detailed distribution patterns of Pb in mice organs (2019)
  • Author(s): Shouta M.M. Nakayama, Masao Togao, Yoshinori Ikenaka, Yoshiki Makino, Ayano Kubota, Takehisa Matsukawa, Kazuhito Yokoyama, Takafumi Hirata, Mayumi Ishizuka
  • Organizer: SETAC North America 40th Annual Meeting
  • Int'l Joint Research
• [Presentation] DDTによる異物代謝酵素の遺伝子発現撹乱と健康影響評価 (2019)
  • Author(s): 本平 航大, 池中 良徳, Yared Beyene, 中山 翔太, 江口 哲史, 武田 一貴, Victor Wepener, Nico Smit, Johan Van Vuren, 石塚 真由美
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Environmental Lead Exposure and DNA Methylation Pattern in Children from Kabwe, Zambia, (2019)
  • Author(s): Yared Beyene Yohannes, Nakayama Shouta, Yabe John, Toyomaki Haruya, Muzandu Kaampwe, Nakata Hokuto, Ikenaka Yoshinori, Choongo Kennedy, Ishizuka Mayumi
  • Organizer: International CHEMICAL HAZARD Symposium
  • Int'l Joint Research / Invited
• [Presentation] Blood Lead (Pb) Levels and Stable Isotope Ratio among Children, Infants and Mothers in Kabwe, Zambia (2018)
  • Author(s): Shouta MM Nakayama, John Yabe, Haruya Toyomaki , Kaampwe Muzandu, Yared B Yohannes, Hokuto Nakata, Yoshinori Ikenaka, Hazuki Mizukawa, Takeshi Kuritani, Mitsuhiro Nakagawa, Mayumi Ishizuka.
  • Organizer: SETAC North America 39th Annual Meeting
  • Int'l Joint Research