Development of multifunctional electron/nuclear magnetic resonance imaging

• Project/Area Number: 18KK0304
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 90:Biomedical engineering and related fields
• Research Institution: Hokkaido University
• Principal Investigator: Hirata Hiroshi 北海道大学, 情報科学研究院, 教授 (60250958)
• Co-Investigator(Kenkyū-buntansha): 市川 和洋 長崎国際大学, 薬学部, 教授 (10271115) ; 榎本 彩乃 長崎国際大学, 薬学部, 講師 (30826186)
• Project Period (FY): 2018-10-09 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2021: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 磁気共鳴イメージング / 電子スピン / 核スピン / pH / 酸素分圧 / 酸化還元状態 / 電子スピン共鳴 / オーバーハウザーMRI / 腫瘍 / レドックス / 酸素 / 核磁気共鳴 / 共振器 / オーバーハウザー効果 / イメージング

Outline of Final Research Achievements

We aimed to develop multiple imaging techniques for the partial pressure of oxygen, extracellular pH, and reduction-oxidation (redox) status using magnetic resonance techniques. This program was conducted internationally with Prof. Valery V. Khramtsov at West Virginia University, WV, USA. In Japan, each imaging technique for electron spins and nuclear spins was developed to visualize malignant tumors’ physiological parameters. The developed imaging techniques were tested and demonstrated in their feasibility studies.

Academic Significance and Societal Importance of the Research Achievements

本課題の学術的意義は、悪性腫瘍の生物学的情報を多面的な方法を用いて可視化する技術を開発することにある。個別のイメージング技術で多種類の情報を同時に可視化することは一般的に困難であるため、電子スピンおよび核スピンによる複数のイメージング技術の開発に取り組んだ。また、悪性腫瘍を多面的に可視化できるイメージング技術は、悪性腫瘍とその治療に関連する研究に貢献することができる。そのため、日本国内で死亡原因の一位である悪性腫瘍の研究に、イメージング技術の面から貢献するところに社会的意義がある。

Research Products

• [Int'l Joint Research] West Virginia University(米国)
• [Int'l Joint Research] West Virginia University(米国)
• [Int'l Joint Research] West Virginia University(米国)
• [Int'l Joint Research] ウエストバージニア大学(米国)
• [Journal Article] RF/Microwave Resonators for Preclinical and Clinical EPR Applications: Current Status and Challenges (2021)
  • Author(s): Hirata Hiroshi、Swartz Harold M.
  • Journal Title: Applied Magnetic Resonance Volume: 53 Issue: 1 Pages: 167-191
  • DOI: 10.1007/s00723-021-01413-3
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Redox-sensitive mapping of a mouse tumor model using spares projection sampling of electron paramagnetic resonance (2021)
  • Author(s): K. Kimura, N. Iguchi, H. Nakano, H. Yasui, S. Matsumoto, O. Inanami, H. Hirata
  • Journal Title: Antioxidants and Redox Signaling Volume: － Issue: 1-3 Pages: 57-69
  • DOI: 10.1089/ars.2021.0003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In vitro simultaneous mapping of the partial pressure of oxygen, pH and inorganic phosphate using electron paramagnetic resonance (2020)
  • Author(s): A. Taguchi, S. DeVience, B. Driesschaert, V. V. Khramtsov, H. Hirata
  • Journal Title: The Analyst Volume: 145 Issue: 9 Pages: 3236-3244
  • DOI: 10.1039/d0an00168f
  • NAID: 120007032611
  • Peer Reviewed / Int'l Joint Research
• [Presentation] EPR-based pH measurements using multiple harmonic detections (2021)
  • Author(s): R. Nakaoka and H. Hirata
  • Organizer: 22nd International Society of Magnetic Resonance Conference (ISMAR)
  • Int'l Joint Research
• [Presentation] Simultaneous mapping of the partial pressure of oxygen, pH and inorganic phosphate using electron paramagnetic resonance (2021)
  • Author(s): A. Taguchi, S. DeVience, B. Driesschaert, V. V. Khramtsov, and H. Hirata
  • Organizer: 22nd International Society of Magnetic Resonance Conference (ISMAR)
  • Int'l Joint Research / Invited
• [Presentation] Simultaneous mapping of the partial pressure of oxygen and pH using electron paramagnetic resonance (2021)
  • Author(s): H. Hirata
  • Organizer: Modern Development of Magnetic Resonance 2021
  • Int'l Joint Research / Invited
• [Presentation] Simultaneous mapping of the partial pressure of oxygen, pH and inorganic phosphate using electron paramagnetic resonance: in vitro experiments (2021)
  • Author(s): A. Taguchi, S. DeVience, B. Driesschaert, V. V. Khramtsov, and H. Hirata
  • Organizer: International Society on Oxygen Transport to Tissue 2021 (ISOTT 2021)
  • Int'l Joint Research
• [Presentation] 圧縮センシングによる3次元CW-EPRイメージングの画像品質評価 (2020)
  • Author(s): 木村洸太, 平田拓
  • Organizer: 第59回電子スピンサイエンス学会年会
• [Presentation] マルチハーモニックデジタル位相検波法による750 MHz EPR分光装置の高感度化 (2020)
  • Author(s): 中岡梨々子, 平田拓
  • Organizer: 第59回電子スピンサイエンス学会年会
• [Presentation] In vivo three-dimensional extracellular pH mapping of tumors using EPR (2019)
  • Author(s): H.Hirata
  • Organizer: 21st ISMAR - 15th EUROMAR Joint Conference
  • Int'l Joint Research / Invited
• [Presentation] In vivo extracellular pH mapping of tumor mouse models using EPR (2019)
  • Author(s): D. A. Komarov, Y. Ichikawa, K. Yamamoto, N. J. Stewart, S. Matsumoto, H. Yasui, I. A. Kirilyuk, V. V. Khramtsov, O. Inanami, H. Hirata
  • Organizer: XI-th Conference of the European Federation of EPR groups
  • Int'l Joint Research
• [Presentation] Recent Progress in 3D Extracellular pH mapping of Tumors using EPR (2019)
  • Author(s): H. Hirata
  • Organizer: International Conference Magnetic Resonance: Current state and future perspectives (EPR-75)
  • Int'l Joint Research / Invited
• [Presentation] OMRI可視化範囲拡大のための多チャンネルEPR励起用コイルアレイの開発 (2019)
  • Author(s): 榎本彩乃, 市川和洋
  • Organizer: 第58回電子スピンサイエンス学会年会
• [Remarks] The Catalyst
  • URL: https://medicine.hsc.wvu.edu/media/366018/catalyst-w2018s2019.pdf