| Project/Area Number |
19109006
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto University |
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Principal Investigator |
NAKAHATA Tatsutoshi Kyoto University, 物質-細胞統合システム拠点, 特定拠点教授 (20110744)
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| Co-Investigator(Kenkyū-buntansha) |
平家 俊男 京都大学, 医学研究科, 准教授 (90190173)
梅田 雄嗣 京都大学, 医学研究科, 助教 (80397538)
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| Co-Investigator(Renkei-kenkyūsha) |
HEIKE Toshio 京都大学, 医学研究科, 教授 (90190173)
UMEDA Katsutsugu 京都大学, 医学研究科, 助手 (80397538)
SAITOH Megumu 京都大学, 物質-細胞統合システム拠点, 特定拠点助教 (90535486)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥109,200,000 (Direct Cost: ¥84,000,000、Indirect Cost: ¥25,200,000)
Fiscal Year 2009: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2008: ¥32,890,000 (Direct Cost: ¥25,300,000、Indirect Cost: ¥7,590,000)
Fiscal Year 2007: ¥50,960,000 (Direct Cost: ¥39,200,000、Indirect Cost: ¥11,760,000)
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| Keywords | ES細胞 / ヒト / 再生医療 / 幹細胞 / NOGマウス |
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| Research Abstract |
We attempt to discover the culture system for generation of desired lineage-specific stem cells from human ES cells and succeeded in generating progenitor cells for hematopoietic cells, endothelial cells, skeletal muscle, cardiomyocytes and neural cells.
VEGFR-2(+)CD34(+) cells are identified as a candidate of primate hemangioblasts. We established a novel protocol to derive myogenic precursors from murine ES/iPS cells with a monoclonal antibody SM/C-2.6 that recognizes quiescent satellite cells. SM/C-2.6-positive cells are highly myogenic and efficiently differentiate into myofibers both in vitro and in vivo. We recently developed culture system for skeletal muscle cells from human ES cells.
Recently, we developed an excellent humanized model mouse, NOG mouse, in which successful engraftment was achieved even if small number of CD34^+ cells were transplanted into the mice. In this study we confirmed that NOG mouse was useful for evaluation of ES/iPS cell-derived tissue specific stem cells.
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