|Budget Amount *help
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2010: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2009: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2008: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2007: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
This study aimed development of handling technology especially for problematic protein using chemical conjugation techniques and protein crystallization methodology. Novel chemical modification reagent and optimization of protein preparation techniques provided improved transduction of transcription factor protein into cells, and solubilization of total cellular protein from cancer cells. Together with a newly developed protein crystallization tag sequence, these technologies will give a useful methodology for structure and functional analysis of proteins and their medical applications.