|Budget Amount *help
¥50,830,000 (Direct Cost: ¥39,100,000、Indirect Cost: ¥11,730,000)
Fiscal Year 2010: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2008: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2007: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
We pursued structural studies of signaling proteins important in cell biology and enzymes that play a role in maintenance of genome stability. Using X-ray crystallography and NMR, we revealed three-dimensional structures of the PH-C1-PH domain of Rho-kinase, the radixin FERM domain-CD44 complex, the PHCCEx domain of RacGEF Tiam1,the CAP-Gly domain of CLIP-170 complexed with the acidic tail of α-tubulin, the gibberellin receptor bound to the effector protein DELLA, the trimeric G protein Gq-YM254890 complex and the WRN-DNA complex and elucidated their structure-function relationships at atomic resolution.