Analyses of the N-substituted formamide metabolic pathway and their applications to the production of useful compounds

• Project/Area Number: 19208008
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: University of Tsukuba
• Principal Investigator: KOBAYASHI Michihiko 筑波大学, 大学院・生命環境科学研究科, 教授 (70221976)
• Co-Investigator(Kenkyū-buntansha): HIGASHIBATA Hiroki 東洋大学, 生命科学部, 准教授 (20344864) ; HASHIMOTO Yoshiteru 筑波大学, 大学院・生命環境科学研究科, 講師 (00323254)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000); Fiscal Year 2009: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2008: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2007: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
• Keywords: N-置換ホルムアミド / イソニトリル / アミド / 代謝 / 酵素

Research Abstract

To clarify the function of enzymes involved in the N-substituted formamide synthesis/degradation, the microorganism with both enzyme activities was analyzed. Isonitrile hydratase was purified and characterize. The enzyme was found to be a new type of isonitrile hydratase. Reverse reaction of N-substituted formamide deformylase was found and the reaction mechanism was proposed. Some compounds were produced using the reverse reaction the enzyme.

Research Products

• [Journal Article] Novel isonitrile hydratase involved in isonitrile metabolism (2010)
  • Author(s): Sato, H., Hashimoto, Y., Fukatsu, H. and Kobayashi, M
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 34793-34802
  • Peer Reviewed
• [Journal Article] Development of novel expression systems for actinomyces (2007)
  • Author(s): Hashimoto, Y
  • Journal Title: Actinomycetol Volume: 21 Pages: 70-75
  • NAID: 40016156752
  • Peer Reviewed
• [Journal Article] イソニトリル・ニトリルの新規代謝経路の発見と新規酵素の分子機能解析 (2007)
  • Author(s): 橋本義輝
  • Journal Title: 生化学 Volume: 79 Pages: 929-940
  • NAID: 10019771773
• [Journal Article] イソニトリル・ニトリルの新規代謝経路の発見と新規酵素の分子機能解析 (2007)
  • Author(s): 橋本義輝
  • Journal Title: 生化学 79 Pages: 929-940
  • NAID: 10019771773
  • Peer Reviewed
• [Journal Article] Development of novel expression systems for actinomyces (2007)
  • Author(s): Hashimoto, Y.
  • Journal Title: Actinomycetol. 21 Pages: 70-75
  • NAID: 40016156752
  • Peer Reviewed
• [Presentation] Arthrobacter属放線菌由来の新規イソニトリルヒドラターゼの解析 (2010)
  • Author(s): 佐藤太祐, 橋本義輝, 小林達彦
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] Arthrobacter属放線菌由来の新規イソニトリルヒドラターゼの解析 (2010)
  • Author(s): 佐藤太祐、橋本義輝、小林達彦
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-29
• [Presentation] 新規イソニトリルヒドラターゼの発見と諸性質の解析 (2009)
  • Author(s): 佐藤太祐, 橋本義輝, 深津寛, 小林達彦
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] 新規イソニトリルヒドラターゼの発見と諸性質の解析 (2009)
  • Author(s): 佐藤太祐、橋本義輝、深津寛、小林達彦
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] 放線菌におけるニトリル関連化合物代謝の応用酵素学的研究 (2009)
  • Author(s): 小林達彦
  • Organizer: 日本放線菌学会
  • Place of Presentation: 秋田
  • Year and Date: 2009-07-16
• [Presentation] Arthrobacter属放線菌由来イソニトリル分解酵素の諸性質の解析 (2009)
  • Author(s): 佐藤太祐, 橋本義輝, 深津寛, 小林達彦
  • Organizer: 日本放線菌学会
  • Place of Presentation: 秋田
  • Year and Date: 2009-07-16
• [Presentation] Arthrobacter属放線菌由来イソニトリル分解酵素の諸性質の解析 (2009)
  • Author(s): 佐藤太祐、橋本義輝、深津寛、小林達彦
  • Organizer: 日本放線菌学会
  • Place of Presentation: 秋田ビューホテル
  • Year and Date: 2009-07-16
• [Presentation] Unique function of the enzyme involved in isonitrile metablism-A new synthetic route to N-benzylamides- (2009)
  • Author(s): M. Kobayashi, T. Sakashita, H. Fukatsu, and Y. Hashimoto
  • Organizer: Biochemical Engineering XVI
  • Place of Presentation: バーリントン(Burlington) USA
  • Year and Date: 2009-07-08
• [Presentation] Biotransformation of benzylamine to N-benzylformamide (2009)
  • Author(s): T.Sakashita, Y. Hashimoto, H. Fukatsu, and M. Kobayashi
  • Organizer: 109^<th> ASM General Meeting
  • Place of Presentation: ペンシルバニアUSA
  • Year and Date: 2009-05-18
• [Presentation] Arthrobacter pascens F164株由来イソニトリルヒドラターゼの精製 (2009)
  • Author(s): 佐藤太祐, 橋本義輝, 深津寛, 小林達彦
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] N-置換ホルムアミド分解酵素の逆反応によるN-置換有機酸アミドの生成 (2009)
  • Author(s): 坂下俊秀, 橋本義輝, 深津寛, 小林達彦
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] 放線菌のN-置換ホルムアミド分解酵素の逆反応解析 (2008)
  • Author(s): 坂下俊秀, 深津寛, 橋本義輝, 小林達彦
  • Organizer: 日本放線菌学会
  • Place of Presentation: 山梨
  • Year and Date: 2008-07-10
• [Presentation] Arthrobacter属放線菌のイソニトリル分解酵素の誘導発現の検討 (2008)
  • Author(s): 佐藤太祐, 深津寛, 橋本義輝, 小林達彦
  • Organizer: 日本放線菌学会
  • Place of Presentation: 山梨
  • Year and Date: 2008-07-10
• [Presentation] 放線菌のN-置換ホルムアミド分解酵素の逆反応解析 (2008)
  • Author(s): 坂下俊秀、深津寛、橋本義輝、小林達彦
  • Organizer: 放線菌学会
  • Place of Presentation: 山梨
  • Year and Date: 2008-07-10
• [Presentation] Arthrobacter属放線菌のイソニトリル分解酵素の誘導発現の検討 (2008)
  • Author(s): 佐藤太祐、深津寛、橋本義輝、小林達彦
  • Organizer: 放線菌学会
  • Place of Presentation: 山梨
  • Year and Date: 2008-07-10
• [Presentation] Degradation of isonitrile by isonitrile hydratase coupled with N-substituted formamide deformylase in Arthrobacter pascens (2008)
  • Author(s): H. Sato, Y. Hashimoto, H. Fukatsu and M. Kobayashi
  • Organizer: FEBS & IUBMB
  • Place of Presentation: アテネ
  • Year and Date: 2008-07-03
• [Presentation] Degradation of isonitrile by isonitrile hydratase coupled with N-substituted formamide deformylase in Artihrobacter pascens (2008)
  • Author(s): H. Sato, Y. Hashimoto, H. Fukatsu and M. Kobayashi
  • Organizer: FEBS&IUBMB
  • Place of Presentation: アテネ
  • Year and Date: 2008-07-03
• [Presentation] New Enzymes involved in isonitrile degradation (2008)
  • Author(s): M. Kobayashi, M. Goda, H. Fukatsu and Y. Hashimoto
  • Organizer: i-Bio-2008 World Congress
  • Place of Presentation: 中国杭州
  • Year and Date: 2008-05-18
• [Presentation] New Enzymes Involved in Isonitrile Degradation (2008)
  • Author(s): Michihiko Kobayashi, Masahiko Goda, Hiroshi Fukatsu and Yoshiteru Hashimoto
  • Place of Presentation: 中国杭州
  • Year and Date: 2008-05-18
• [Presentation] Arthrobacter pascens F164株由来イソニトリルヒドラターゼの最適発現条件 (2008)
  • Author(s): 佐藤太祐, 深津寛, 橋本義輝, 小林達彦
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-28
• [Presentation] Arthrobacter pascensF164株由来イソニトリルヒドラターゼの最適発現条件 (2008)
  • Author(s): 佐藤 太祐、深津 寛、橋本 義輝、小林 達彦
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-28
• [Book] 構造生物学-ポストゲノム時代のタンパク質研究-"自然界から有用酵素を収集する方法:イソニトリル代謝関連酵素群" (2007)
  • Author(s): 橋本義輝、小林達彦
  • Publisher: 共立出版