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Inactivation of blackening enzymes in crustaceans by inert gases and prevention of blackening.

Research Project

Project/Area Number 19208020
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionKyoto University

Principal Investigator

HIRATA Takashi  Kyoto University, 大学院・農学研究科, 教授 (40273495)

Co-Investigator(Kenkyū-buntansha) SUGAWARA Tatsuya  京都大学, 大学院・農学研究科, 准教授 (70378818)
MAKINO Yoshio  東京大学, 農学生命科学研究科, 准教授 (70376565)
ITO Shosuke  藤田保健衛生大学, 医療科学部, 教授 (70121431)
WAKAMATSU Kazumasa  藤田保健衛生大学, 医療科学部, 教授 (80131259)
SUGAWARA Tatsuya  京都大学, 大学院・農学研究科, 助教 (40395653)
ADACHI Kosuke  高知大学, 教育研究部自然科学系, 准教授 (00399114)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000)
Fiscal Year 2010: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2009: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2008: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2007: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Keywordsメラニン / 二酸化炭素 / エビ / 黒変 / フェノール酸化酵素 / ホルムアルデヒド / フェノールオキシダーゼ / 包装 / ビブリオ / 甲殻類 / 酵素 / 食品 / 水産学 / 農林水産物 / ヘモシアニン
Research Abstract

Blackening in crustaceans was controlled under carbon dioxide (CO_2). The structure in the vicinity of active site of the blackening enzyme may be altered by the action of CO_2. A novel enzyme was cloned from plasma of kuruma prawn Marsupenaeus japonicus. Formaldehyde was not observed in the prawn during storage under CO_2. Blackening of the prawn was accompanied by the loss of functional components. The growth of Vibrio parahaemolyticus in Alaskan pink shrimp Pandalus eous was effectively suppressed under CO_2. These results showed that CO_2 is a useful tool for prevention of blackening together with suppression of growth of food poisoning Vibrio.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (37 results)

All 2011 2010 2009 2008 Other

All Journal Article (8 results) (of which Peer Reviewed: 3 results) Presentation (12 results) Book (14 results) Remarks (3 results)

  • [Journal Article] Mathematical analysis for growth depression of Vibrio parahaemolyticus in shrimp under a high carbon dioxide atmosphere2011

    • Author(s)
      Makino Y., Ito Y., Oshita S., Kawagoe Y., Kawabuchi Y., Sugawara T., Hirata T.
    • Journal Title

      Food Science and Technology Research 17(1)

      Pages: 63-67

    • NAID

      10027868626

    • Related Report
      2010 Final Research Report
  • [Journal Article] Mathematical analysis for growth depression of Vibrio parahaemolyticus in shrimp under a high carbon dioxide atmosphere2011

    • Author(s)
      Makino Y., Ito Y., Oshita S., Kawagoe Y., Kawabuchi Y., Sugawara T., Hirata T.
    • Journal Title

      Food Science and Technology Research

      Volume: 17(1) Pages: 63-67

    • NAID

      10027868626

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regioselectivity on the cooxidation of 5, 6-dihydroxyindole and its 2-carboxy derivative from the quantum chemical calculations.2010

    • Author(s)
      Okuda H, Wakamatsu K, Ito S, Sota T.
    • Journal Title

      Chem Phys Lett (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical analysis of late stages of pheomelanogenesis : conversion of dihydrobenzothiazine to a benzothiazole structure.2009

    • Author(s)
      Wakamatsu, K., Ohtara, K., Ito, S.
    • Journal Title

      Pigment Cell & Melanoma Res. 22

      Pages: 474-486

    • Related Report
      2010 Final Research Report
  • [Journal Article] Current challenges in understanding melanogenesis : bridging chemistry, biological control, morphology, and function2009

    • Author(s)
      Simon JD, Peles D, Wakamatsu K, Ito S.
    • Journal Title

      Pigment Cell Melanoma Res (Review) 22

      Pages: 563-579

    • Related Report
      2010 Final Research Report
  • [Journal Article] Current challenges in understanding melanogenesis : bridging chemistry, biological control, morphology, and function2009

    • Author(s)
      Simon JD, Peles D, Wakamatsu K, Ito S.
    • Journal Title

      Pigment Cell Melanoma Res 22

      Pages: 563-579

    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] 凍結解凍後のエビ類における黒色化の防除策2009

    • Author(s)
      足立亨介
    • Journal Title

      冷凍 (冷凍空調学会誌) 84

      Pages: 41-44

    • NAID

      10026351434

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] 凍結解凍後のエビ類における黒色化の防除策2009

    • Author(s)
      足立亨介
    • Journal Title

      冷凍(冷凍空調学会誌) 84

      Pages: 41-44

    • NAID

      10026351434

    • Related Report
      2009 Annual Research Report
  • [Presentation] クルマエビの黒変現象に関わる新規因子の単離とその性質2010

    • Author(s)
      殿本将司, 大友良祐, 増田太郎, 菅原達也, 平田孝
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都大学100周年時計台記念館(京都市)
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] エビに付着した腸炎ビブリオ菌の増殖抑制を目的とするCO_2置換包装に関する研究2010

    • Author(s)
      伊藤友樹, 牧野義雄, 大下誠一, 川越義則, 川渕幸映, 綾木毅, 田中幹雄, 広瀬和彦, 菅原達也, 平田孝
    • Organizer
      第69回農業機械学会年次大会
    • Place of Presentation
      愛媛大学農学部(松山市)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Predictive food microbiology on growth depression of Vibrio parahaemolyticus in pink shrimp under CO_22010

    • Author(s)
      伊藤友樹, 牧野義雄, 大下誠一, 川越義則, 川渕幸映, 菅原達也, 平田孝
    • Organizer
      International Conference on Agricultural Engineering AgEng 2010
    • Place of Presentation
      ポリドーム(クレルモンフェラン市,フランス)
    • Year and Date
      2010-09-08
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] CO_2置換包装によるエビに付着した腸炎ビブリオ菌の増殖抑制に関する研究2010

    • Author(s)
      伊藤友樹, 牧野義雄, 大下誠一, 川越義則, 川渕幸映, 綾木毅, 田中幹雄, 広瀬和彦, 菅原達也, 平田孝
    • Organizer
      日本包装学会第19回年次大会研究発表会
    • Place of Presentation
      東京大学弥生講堂(東京)
    • Year and Date
      2010-07-08
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Effects of carbon dioxide on postharvest blackening of Alaskan Pink ShrimpPandalus eous2009

    • Author(s)
      平田孝, 菅原達也, 綾木毅, 田中幹雄, 広瀬和彦
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-10-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Effects of carbon dioxide on postharvest blackening of Alaskan Pink Shrimp Pandalus eous2009

    • Author(s)
      T. Hirata, T. Sugawara, T. Ayaki, M. Tanaka, K. Hirose
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-10-23
    • Related Report
      2009 Self-evaluation Report 2008 Annual Research Report
  • [Presentation] エビに付着した腸炎ビブリオ菌のCO_2を利用した増殖抑制に関する予測微生物学的研究2009

    • Author(s)
      伊藤友樹, 牧野義雄, 大下誠一, 川越義則, 川渕幸映, 平田孝
    • Organizer
      農業環境工学関連学会2009年合同大会
    • Place of Presentation
      東京大学教養学部(東京)
    • Year and Date
      2009-09-18
    • Related Report
      2010 Final Research Report
  • [Presentation] エビに付着した腸炎ビブリオ菌のCO2を利用した増殖抑制に関する予測微生物学的研究2009

    • Author(s)
      伊藤友樹, 牧野義雄, 大下誠一, 川越義則, 川渕幸映, 平田孝
    • Organizer
      農業環境工学関連学会2009年合同大会
    • Place of Presentation
      東京大学教養学部
    • Year and Date
      2009-09-18
    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] 調整気相下におけるエビ汚染腸炎ビブリオ増殖の予測微生物学モデリング2009

    • Author(s)
      牧野義雄、伊藤友樹、大下誠一、川越義則、川渕幸映、平田孝
    • Organizer
      日本食品工学会第10回年次大会
    • Place of Presentation
      石川県立大学(石川県野々市町)
    • Year and Date
      2009-08-02
    • Related Report
      2010 Final Research Report
  • [Presentation] エビに付着した腸炎ビブリオ菌のCO_2を利用した増殖抑制に関する予測微生物学的研究2009

    • Author(s)
      伊藤友樹、牧野義雄、大下誠一、川越義則、川渕幸映、平田孝
    • Organizer
      農業機械学会関東支部年次大会
    • Place of Presentation
      千葉大学園芸学部(松戸市)
    • Year and Date
      2009-07-11
    • Related Report
      2010 Final Research Report
  • [Presentation] エビに付着した腸炎ビブリオ菌のCO2を利用した増殖抑制に関する予測微生物学的研究2009

    • Author(s)
      伊藤友樹, 牧野義雄, 大下誠一, 川越義則, 川渕幸映, 平田孝
    • Organizer
      農業機械学会関東支部年次大会
    • Place of Presentation
      千葉大学園芸学部
    • Year and Date
      2009-07-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホッコクアカエビの黒変におよぼす二酸化炭素の影響2009

    • Author(s)
      大友良祐, 若木良大, 菅原達也, 平田孝, 綾木毅, 田中幹雄, 広瀬和彦
    • Organizer
      日本包装学会第18会年次大会
    • Place of Presentation
      東京大学弥生講堂
    • Year and Date
      2009-07-09
    • Related Report
      2010 Final Research Report 2009 Annual Research Report 2009 Self-evaluation Report
  • [Book] Blackspot development on crustaceans during storage(Handbook of Seafood Safety, Quality and Health Applications)(Ed Shahidi, Cerarettin, Miyashita)2011

    • Author(s)
      Kohsuke Adachi, Takashi Hirata
    • Publisher
      Wiley-Blackwell Publisher
    • Related Report
      2010 Final Research Report
  • [Book] Blackening of crustaceans during storage : mechanism and prevention, In "Handbook of Seafood Quality, Safety and Health Applications"2010

    • Author(s)
      Kohsuke Adachi, Takashi Hirata
    • Total Pages
      10
    • Publisher
      Wiley-Blackwell
    • Related Report
      2010 Annual Research Report
  • [Book] Seafood Safety and Quality(Blackspot development on crustaceans during storage)2010

    • Author(s)
      Kohsuke Adachi, Takashi Hirata
    • Publisher
      Blackwell Publisher(in press)
    • Related Report
      2009 Self-evaluation Report
  • [Book] Blackspot development on crustaceans during storage (in Seafood Safety and Quality)2010

    • Author(s)
      Kohsuke Adachi, Takashi Hirata
    • Publisher
      Blackwell Publisher(in press)
    • Related Report
      2009 Annual Research Report
  • [Book] 凍結解凍後のエビ類における黒色化の防除策、冷凍2009

    • Author(s)
      足立亨介
    • Publisher
      冷凍空調学会誌84(985)
    • Related Report
      2010 Final Research Report
  • [Book] 水産物の色素2008

    • Author(s)
      平田孝・菅原達也編
    • Total Pages
      127
    • Publisher
      恒星社厚生閣
    • Related Report
      2010 Final Research Report
  • [Book] 食品の創造(第3章)(安達修二編)(水産物の色)2008

    • Author(s)
      平田孝
    • Publisher
      京都大学学術出版会
    • Related Report
      2010 Final Research Report
  • [Book] 水産物の色素(平田孝・菅原達也編)(メラニン色素の化学)2008

    • Author(s)
      伊藤祥輔、若松一雅
    • Publisher
      恒星社厚生閣
    • Related Report
      2010 Final Research Report
  • [Book] 水産物の色素(平田孝・菅原達也編)(メラニン色素の化学)(メラニン生成による甲殻類の黒変と品質)2008

    • Author(s)
      平田孝、足立亨介
    • Publisher
      恒星社厚生閣
    • Related Report
      2010 Final Research Report
  • [Book] 水産物の色(食品の創造 安達修二編 第3章)2008

    • Author(s)
      平田孝
    • Publisher
      京都大学学術出版会
    • Related Report
      2009 Self-evaluation Report 2008 Annual Research Report
  • [Book] メラニン色素の化学(平田孝・菅原達也編, 水産物の色素)2008

    • Author(s)
      伊藤祥輔, 若松一雅
    • Publisher
      恒星社厚生閣
    • Related Report
      2009 Self-evaluation Report
  • [Book] メラニン生成による甲殻類の黒変と品質(平田孝・菅原達也編, 水産物の色素)2008

    • Author(s)
      平田孝, 足立亨介
    • Publisher
      恒星社厚生閣
    • Related Report
      2009 Self-evaluation Report
  • [Book] 水産物の黒色変化に関する生化学的解析

    • Author(s)
      足立亨介
    • Publisher
      日本水産学会誌(印刷中)
    • Related Report
      2010 Final Research Report
  • [Book] 水産物の色素

    • Author(s)
      平田孝・菅原達也
    • Publisher
      恒星社厚生閣
    • Related Report
      2007 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.bioproducts.marine.kais.kyoto-u.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.bioproducts.marine.kais.kyoto-u.ac.jp/

    • Related Report
      2009 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.jimu.kais.kyoto-u.ac.jp/wiki-gakugai/index.php?plugin=attach&refer%B9%AD%CA%F3%B0%D1%B0%F7%B2%F1%A1%A6%B8%A6%B5%E6%B0%C6%C6%E2PDF%2F4%B1%FE%CD%D1%C0%B8%CA%AA%B2%CA%B3%D8%C0%EC%B9%B6&openfile%B3%A4%CD%CE%C0%B8%CA%AA%C0%B8%BB%BA%CD%F8%CD%D1%B3%D8_new.pdf

    • Related Report
      2009 Self-evaluation Report

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Published: 2007-04-01   Modified: 2016-04-21  

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