A role of Na^+-K^+ATPase (Na^+pump) on signaling proteins of action anesthetic and its structural change

• Project/Area Number: 19209050
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Gifu University
• Principal Investigator: DOHI Shuji (2009) Gifu University, 大学院・医学系研究科, 教授 (80136952); 土肥 修司 (2007-2008) Gifu University, 大学院・医学系研究科, 教授 (40155627)
• Co-Investigator(Kenkyū-buntansha): TANABE Kumiko 岐阜大学, 大学院・医学系研究科, 講師 (30402209) ; YANAGIDATE Fumi 岐阜大学, 医学部附属病院, 助教 (60313889) ; SUGIYAMA Youoko 岐阜大学, 大学院・医学系研究科, 助教 (70444255)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000); Fiscal Year 2009: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2008: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000); Fiscal Year 2007: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: Na^+-K^+ATPase / Na^+-K^+-2CL^- / 局所麻酔 / 脊髄疼痛受容体機構 / ERK / イオントランスポーター / グリア細胞 / 麻酔 / GDNF / Na^+-K^+-2CL^-. / 局所麻酔薬 / 脊髄疼痛受容機構 / ATP感受性Kチャネル / 麻酔薬 / NA+-K+ATPase / CCC / 細胞内シグラル伝達 / MAPキナーゼ

Research Abstract

Na^+-K^+ATPaseは細胞内外のNa^+とK^+の濃度を調節・制御しており、麻酔薬によるシグナル伝達機構にどう影響するかを、麻酔作用の重要なターゲットである脊髄後根神経節ニューロン(感覚ニューロン)および脊髄後角ニューロンにおいて検討した。Na^+-K^+ATPaseもイオントランスポーターであるCation-Chloride Cotransporters(CCC)も、エンドセリンも麻酔薬の脊髄の疼痛シグナル制御機構にさまざまな影響を与え、正常と損傷を受けた動物とによって異なること、グリア細胞もその作用の一端を担っていることを示唆する結果を得た。

Research Products

• [Journal Article] Mechanisms of tumor necrosis factor-α-induced interleukin-6 synthesis in glioma cells (2010)
  • Author(s): Tanabe K, Yamaguchi S, Dohi S, 他3名
  • Journal Title: Journal of Neuroinflammation 7 Pages: 16-24
  • NAID: 120006341942
  • Peer Reviewed
• [Journal Article] Comparative effects of ultra-short-acting β1-blockers on voltage-gated tetrodotoxin-resistant Na+ channels in rat sensory neurons. (2009)
  • Author(s): Tanahashi S, Iida H, Dohi S, Oda A, Osawa Y, Yamaguchi S
  • Journal Title: Eur J Anaesth 26 Pages: 196-200
  • Peer Reviewed
• [Journal Article] Mechanisms of interleukin-1β-induced GDNF release from rat glioma cells. (2009)
  • Author(s): Tanabe K, Nishimura K, Dohi S, Kozawa O
  • Journal Title: Brain Reserch 1274 Pages: 11-20
  • Peer Reviewed
• [Journal Article] Involvement of Rho-kinase in tumor necrosis factor-α-induced interleukin-6 release from C6 glioma cells. (2009)
  • Author(s): Yamaguchi S, Tanabe K, Takai S, Matsushima-Nishiwaki R, Adachi S, Iida H, Kozawa O, Dohi S
  • Journal Title: Neurochemistry International 55 Pages: 438-445
  • Peer Reviewed
• [Journal Article] Mechanisms of interleukin-1 β-indeced GDNF release from rat glioma cells (2009)
  • Author(s): Tanabe K, Nishimura K, Dohi Shuji, Kozawa O
  • Journal Title: Brain Research 1274 Pages: 11-20
  • Peer Reviewed
• [Journal Article] Involvement of Pho-kinase in tumor necrosis factor-α-induced interleukin-6 relaese from C6 glioma cells (2009)
  • Author(s): Yanaguchi S, Tanabe K, Dohi S, 他5名
  • Journal Title: Neurochemistry International 55 Pages: 438-445
  • Peer Reviewed
• [Journal Article] α2 adrenoreceptor agonist regulates protein kinase C-induced heat shock protein 27 phosphorylation in C6 glioma cells. (2008)
  • Author(s): Tanabe K, Takai S, Matsushima-Nishiwaki R, Kato K, Dohi S, Kozawa O
  • Journal Title: J Neurochem 106 Pages: 519-528
  • Peer Reviewed
• [Journal Article] Nicorandil, an ATPSensitive Potassium Channel Opener, Inhibits Muscarinic Acetylcholine Receptor-Mediated Activation of Extracellular Signal-Regulated Kinases in PC12 Cell. (2008)
  • Author(s): Niinomi K, Banno Y, Iida H, Dohi S
  • Journal Title: Anesth Analg 107 Pages: 1892-1898
  • Peer Reviewed
• [Journal Article] a_2 adrenoreceptor agonist regulates protein kinase C-induced heat shock protein 27 phosphorylation in C6 glioma cells. (2008)
  • Author(s): Kumiko Tanabe, Shinji Takai, Matsushima-Nishiwaki, Kanef usa Kato, Shuji Dohi, Osamu Kozawa
  • Journal Title: 1. J Neurochem 106 Pages: 519-528
  • Peer Reviewed
• [Journal Article] Nicorandil, an Adenosine Triphosphate-Sensitive Potassium Channel Opener, Inhibits Muscarinic Acetylcholine Receptor-Mediated Activation of Extracellular Signal-Regulated Kinases in PC12 Cell. (2008)
  • Author(s): Niinomi K, Banno Y, Lida H, Dohi s.
  • Journal Title: 2. Anesth Analg 2008 ; 107:1892-1898 107 Pages: 1892-1898
  • Peer Reviewed
• [Journal Article] The effects of transient cerbral ischemia on vasopressin-induced vasoconstriction in rabbit cerebral vessels. 2008 mar : 106(3) : 910-915 (2008)
  • Author(s): Kumazawa M, lida II, Uchida M, lida M, Fukuoka N, Michino T, Dohi S.
  • Journal Title: Anesth Analg. 106 Pages: 910-915
  • Peer Reviewed
• [Journal Article] Nicorandil, a K+ATP channel Opener, Inhibits Muscarinic Activation of Extracellular Signal-Regulated Kinases (ERK) in PC12 cells. (2008)
  • Author(s): Niinomi K, Banno Y, Iida H, Dohi S:
  • Journal Title: Anesthesia Analgesia 誌に2008年掲載決定
  • Peer Reviewed
• [Journal Article] α 2 Adrenoreceptor agonist regulates protein kinase C-induced he atshock protein 27 phosphorylation in C6 glioma cells. (2008)
  • Author(s): Tanabe T, Takai S, Dohi S, 他4名
  • Journal Title: J Neurochemistry誌に2008年に掲載決定
  • Peer Reviewed
• [Journal Article] Antinociceptive synergistic interaction between clonidine and ouabain on thermal nociceptive tests in the rat. (2007)
  • Author(s): Zeng W, Chen X, Dohi S
  • Journal Title: J Pain 8 Pages: 983-988
  • Peer Reviewed
• [Journal Article] Effects of alpha2-adrenoceptor agonists on tetrodotoxin-resistant Na+ channels in rat dorsal root ganglion neurons. (2007)
  • Author(s): Oda A, Iida H, Tanahashi S, Osawa Y, Yamaguchi S, Dohi S
  • Journal Title: Eur J Anaesthesiol 24 Pages: 934-941
  • Peer Reviewed
• [Journal Article] Effects of ifenprodil on valtage-gated tetrodtoxin-resistant Na+ Channels in rat sensory neurons. (2007)
  • Author(s): Tanahashi S, Iida H, Oda A, Osawa Y, Uchida M, Dohi S
  • Journal Title: Eur J Anaesth 24 Pages: 782-788
  • Peer Reviewed
• [Presentation] Tumor necrosis factor-aαによるグリア細胞からのInterleukin-6の産生機序 (2010)
  • Author(s): 田辺久美子
  • Organizer: 日本麻酔科学会第57回学術集会
  • Place of Presentation: 福岡
• [Presentation] 局所麻酔薬の脊髄後角細胞における作用機序、ERK活性に及ぼす影響 (2009)
  • Author(s): 張連喜, 柳舘富美, 陳桂花, 土肥修司
  • Organizer: 日本麻酔科学会第56回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2009-05-24
• [Presentation] エンドセリン(ET-1)の脊髄における鎮痛作用の検討 (2009)
  • Author(s): 陳桂花, 柳舘富美, 張連喜, 土肥修司
  • Organizer: 日本麻酔科学会第56回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2009-05-22
• [Presentation] A role of Na+-K+-2Cl- cation-chloride cotransporters on spinal pain transmission (2009)
  • Author(s): Yanagidate F, He Y, Chen GH, Zhang L, Kawasaki Y, Dohi S
  • Organizer: 9th European Meeting on Glial Cells in Health and Disease
  • Place of Presentation: Paris
• [Presentation] Mechanisms of interleukin-1β-induced GDNF release from rat glioma cells. (2009)
  • Author(s): Tanabe K, Dohi S, Yanagidate F, Kozawa O
  • Organizer: 9th European Meeting on Glial Cells in Health and Disease
  • Place of Presentation: Paris
• [Presentation] グリア細胞におけるprotein kinase CにおけるHSP27リン酸化に対するdexmedetomidineの作用 (2008)
  • Author(s): 田辺久美子
  • Organizer: 日本麻酔科学会第55回学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-12
• [Presentation] Interleukin 1βによるグリア細胞からのグリア細胞由来神経成長因子(GDNF)遊離機序 (2008)
  • Author(s): 田辺久美子
  • Organizer: 日本麻酔科学会第56回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2008-05-22
• [Presentation] Nicorandil, an ATPSensitive Potassium Channel Opener, Inhibits Muscarinic Acetylcholine Receptor-Mediated Activation of Extracellular Signal-Regulated Kinases in PC12 Cell. (2008)
  • Author(s): Niinomi K, Banno Y, Iida H, Dohi S
  • Organizer: IARS meeting at Orland
  • Place of Presentation: Florida
  • Year and Date: 2008-03-23
• [Presentation] グリア細胞におけるprotein kinase CによるHSP27リン酸化に対するdexmedetomidineの作用. (2008)
  • Author(s): 田辺久美子, 土肥修司
  • Organizer: 日本麻酔科学会第55回学術集会
  • Place of Presentation: 横浜市
• [Book] in Perioperative Complications in Neurosurgical Anesthesia and Critical Care Medicine Strategies for preventin, early detecton, and management. (2010)
  • Author(s): Dohi S
  • Publisher: Complications during Ptuitary Surgery
• [Book] 土肥編集別冊医学のあゆみ「麻酔科学のUPDATE」2009、麻酔中の覚醒と夢 (2009)
  • Author(s): 土肥修司
  • Publisher: 医歯薬出版